Thesis (Ph. D.)--Johns Hopkins University, 1933. / Vita. "Sonderabdruck aus dem Archiv für protistenkunde, bd. 84, 1934." "Literature cited": p. 30-32.

Gerbils, experimentally infected with Giardia lamblia trophozoites, had trophozoites and encysting trophozoites in all 3 equal sections of the small intestine and in the colon at necropsy. Cysts were found in the second and third sections of the small intestine and in the colon. WB strain derived trophozoites (WB, D1, WB-C6, and V1) differed in levels of encystation in vitro but not in gerbils. Passage in gerbils increased the in vitro encystation levels of WB and D1 but decreased that of WB-C6 and V1. No differences were found in the total protein profiles or isoenzyme patterns of these G. lamblia populations. Immunization of mice with in vitro cysts produced monoclonal antibodies (mAbs) recognizing cyst protein antigens. In Immunofluorescence (IFA), mAb 5A4.G6 recognized cyst walls. This mAb reacted with a 38 kD band on Western blots. IFA results showed that mAb 8C5.C11 reacts with vesicles in encysting trophozoites and with cyst walls. It recognized 26, 28, 42 and 46 kD bands in Western blots. When mAb 8C5.C11 and Guinea pig complement were added to 0-9 hour encysting cultures, the numbers of cysts produced were significantly reduced compared to control. MAb 5A4.G6 did not affect in vitro encystation.

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