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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Efeito da administração da Deslorelina por 70 dias na forma de implante na dinâmica folicular, perfil endócrino e expressão de receptores endométriais de estrógeno, progesterona e ocitocina de vacas nelore (Bos taurus indicus) /

Marques Filho, Wolff Camargo. January 2011 (has links)
Orientador: João Carlos Pinheiro Ferreira / Banca: Nereu Carlos Prestes / Banca: Sony Dimas Bicudo / Banca: Inês Cristina Giometti / Banca: Jeanne Broch Siqueira / Resumo: O objetivo deste foi avaliar os efeitos da utilização de implantes subcutâneos de Deslorelina (agonista de GnRH-Suprelorin®) por 70 dias sob a dinâmica folicular, secreção de FSH, LH e P4 e, a expressão gênica e proteica dos receptores endometriais de estrógeno e (E R e E R), de progesterona isoformas A e B (PABR e PBR) e de ocitocina (OTR) em vacas Nelore (Bos taurus indicus). Foi sincronizada a ovulação de 15 vacas Nelore com escore de condição corporal (ECC) 3 e, no D0 foram aleatoriamente selecionados 7 animais para serem submetidos a implantação subcutânea de Deslorelina por 70 dias. O exame ultra-sonográfico foi realizado para caracterização da dinâmica folicular e os folículos foram classicados em classes: I (< 0,4 cm), II (0,4-0,6cm) e III (> 0,6cm). Amostras de sangue foram coletadas para mensuração das concentrações plasmáticas de FSH, LH e P4. A pulsatilidade e os picos de LH foram determinados a partir de amostras sanguíneas coletadas a cada 15 minutos por 6 horas em 4 vacas escolhidas aleatoriamente nos D20 e D62. A mensuração da expressão proteica dos E R, E R, PABR e PBR no epitélio glandular e estroma uterino foram mensurados por meio da técnica da imunoistoquímica e, a expressão gênica dos E R, E R, PABR e OTR foi avaliada com auxílio da técnica de qRT-PCR, ambas... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The objective this study is to evaluate the effects of subcutaneous implant Deslorelin (GnRHa-Suprelorin®) by 70 days on the endocrine dynamic, the follicular development and, gene and protein expression of endometrial receptors of estrogen and (E R and E R), progesterone isoforms A and B (PABR and PBR), and oxytocin (OTR) of Nelore (Bos taurus indicus) cows . Ovulation of 15 Nelore cows with BCS 3 was synchronized following of implantation of Deslorelin in 7 animals for 70 days. During this period, the ovaries were scanned by ultrasonography to characterize the follicular dynamics, where the follicles were divided in class: I (< 0.4 cm), II (0.4-0.6 cm) and III (> 0.6 cm). Blood samples were collected before of each ultrasonographic exam for later measurement of plasma FSH, LH and P4. The assessment of LH pulsatile secretion was measured on blood samples collected every 15 minutes for six hours through the jugular catheterization in fourrandomly chosen cows on D20 and D62. The protein expression of E R and E R, PABR and PBR of endometrial glandular epithelial and stromal was measured using the technique of immunohistochemistry and the quantification of gene expression of E R, E R, PABR and OTR by qRT-PCR technique from fragments collected through endometrials biopsies on days 0, 4, 20 and 27. Others two samples of endometrium were used for. The comparison between the moments of the measures was performed using the nonparametrics tests of the Friedman and Fisher's Exact. The relationship between the results of follicular dynamics, gene and protein expression was performed by Pearson correlation, all with a significance level of 0.05. During the 70 days of implantation of Deslorelin neither animal ovulated, indicating that the preovulatory peak of LH was abolished. The maximum number of follicles... (Complete abstract click electronic access below) / Doutor

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