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Adherence of microfilariae of the filarial nematode Brugia malayi to human endothelial cells and their effect on human endothelial cell mediated immune responsesSchroeder, Jan-Hendrik January 2010 (has links)
No description available.
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Structure and function of CD31Newton, Justin Philip January 1997 (has links)
The regulated interaction of leukocyte with endothelium is of key importance during normal immune surveillance and leukocyte infiltration to sites of infection in the inflammatory response. This thesis is concerned with the structure and function of CD31 (platelet-endothelial cell adhesion molecule-1), one of the adhesion molecules implicated in these processes. Previous work has shown both in vivo and in vitro that CD31 is involved in the final step of leukocyte recruitment, transmigration across the endothelial monolayer. CD31 mediated adhesion is complex, since it is capable of mediating multiple adhesive interactions, both to itself (homophilic adhesion) and to other ligands (heterophilic adhesion). In order to study homophilic adhesion, an heterologous cell-protein assay was used in combination with recombinant chimeric CD31Fc fusion proteins, ICAM-3/CD31 chimeras and chimeras between human and murine CD31. These reagents located the homophilic binding site to the NH<sub>2</sub>-terminal domains 1 and 2, but also define a non-binding accessory role for the membrane proximal domains. Using site-directed mutagenesis to target all of the exposed charged residues in domain 1 and a subset of charged residues in domain 2, five residues were identified, mutations in which resulted in inhibition of homophilic adhesion. These residues map to both faces of the domain 1 immunoglobulinlike fold, suggesting that each molecule of CD31 interacts with two others. A novel zipper model of homophilic adhesion involving CD31 lateral association analogous to that seen amongst cadherins is proposed on the basis of these results. Evidence for lateral association of CD31 to form dimers was obtained from biophysical, biochemical and molecular biology techniques. These show that Cd31 exists in an equilibrium between monomeric and dimeric forms both in solution as soluble recombinant protein, and at the cell surface. In solution the affinity of the interaction was calculated to lie in the range 12-14μM. A large panel of anti-CD31 monoclonal antibodies were generated and tested for their ability to effect homophilic adhesion. Inhibitory antibodies were identified, mapping throughout the extracellular domain, away from the ligand binding site. In addition possible stimulating antibodies mapping to the membrane proximal domains were also identified. This indicates that CDS 1 may be induced to undergo conformational changes which effect homophilic adhesion, and it is proposed that these conformational changes may be linked to the ability of CD31 to form laterally associated dimers. Using the reagents described above, a screen of haematopoietic cell lines identified a novel heterophilic interaction, which was shown to be mediated by the integrin αvβ3. Proteinprotein assays were used to confirm a direct physical association between CD31 and αvβ3, and to map the integrin binding site to the third immunoglobulin-like fold of CD31. The functional significance of this interaction was assessed in neutrophil transmigration assays, in which both anti-CD31 and anti-αvβ3 antibodies were found to partially inhibit neutrophil transmigration.
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Expression of vascular endothelial growth factor in dogs with Spirocerca lupi-associated neoplastic transformationMukorera, Varaidzo 22 November 2012 (has links)
Tumour development is dependent upon the formation of an adequate blood supply through angiogenesis. Vascular endothelial growth factor (VEGF) is one of the most potent and specific pro-angiogenic factors associated with tumour development. Vascular endothelial growth factor is elevated in dogs with a variety of neoplastic tumours and has been linked to an increased risk for metastasis and a poorer prognosis in several tumours. Spirocerca lupi (S. lupi) is a nematode of canids which infests the oesophagus where it forms a nodule. The oesophageal nodule can develop into a neoplastic tumour namely osteosarcoma, fibrosarcoma or anaplastic sarcoma. The pathogenesis of the neoplastic transformation is poorly understood. Diagnosis of neoplastic transformation can be challenging and is based on endoscopy-guided biopsies which are invasive, expensive and may yield non diagnostic samples. The aim of this prospective study was to determine if serum and plasma VEGF levels could be used to distinguish between neoplastic and non-neoplastic spirocercosis. Twenty four dogs were enrolled in the study, 9 with non-neoplastic, 9 with neoplastic spirocercosis, and 6 control dogs. Plasma and serum samples for VEGF analysis were collected at diagnosis. Measurement of VEGF was done using a canine VEGF Quantikine ELISA kit. Statistical analysis to compare the means of the VEGF concentrations between the groups was performed using the Kruskal-Wallis followed by the Dunn’s test. Significance was set at p<0.05 The median plasma VEGF concentration of the dogs with neoplastic spirocercosis 629pg/ml (range 282 – 2366) was higher than the median plasma VEGF concentrations of both the non-neoplastic 0pg/ml (range 0 – 716) and controls 0pg/ml (range 0 – 0) (p<0.001). The median serum VEGF concentration of the neoplastic dogs 69pg/ml (range 0 – 212) was higher than the serum VEGF concentrations in the non-neoplastic 0pg/ml (range 0 – 44.13) and control 0pg/ml (range 0 – 39.4) (p=0.001). Plasma VEGF at a cut off value of 250pg/ml was determined to have a sensitivity of 100%, specificity of 77.8%, a PPV of 81.8% and a NPV of 100% for determining neoplastic transformation. Serum VEGF at a cut off value of 25pg/ml was determined to have a sensitivity of 88.9%, specificity of 100%, a PPV of 100% and a NPV of 90% for determining neoplastic transformation. Both plasma and serum VEGF concentrations can be used to differentiate between non-neoplastic and neoplastic spirocercosis. Plasma VEGF concentrations were higher than serum VEGF concentrations, contrary to what is reported in literature. Both plasma and serum VEGF concentrations can, therefore, potentially be used for diagnosis of neoplastic vs. non-neoplastic cases in canine spirocercosis. There is a need to perform more studies to determine cut-off concentrations that would maximize the sensitivity and specificity for determining neoplastic transformation in canine spirocercosis as well as to determine the role of VEGF in the pathogenesis of the neoplastic transformation. Copyright / Dissertation (MMedVet)--University of Pretoria, 2012. / Companion Animal Clinical Studies / unrestricted
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Proteomic Analysis Identifies Translationally Controlled Tumor Protein as a Potential Novel Mediator of Occlusive Vascular Remodeling in Pulmonary Arterial HypertensionLavoie, Jessie January 2013 (has links)
Pulmonary arterial hypertension (PAH) is a lethal disease characterized by excessive
proliferation of pulmonary vascular cells, such as endothelial cells (ECs). Hereditary (H)
PAH is mainly caused by ―loss-of-function‖ mutations in the gene coding for the bone
morphogenetic protein type II receptor (BMPR2). However, the mechanisms by which
these mutations cause PAH remain unclear. The hypothesis of this thesis was that
BMPR2 mutations produce an imbalance in EC protein expression and/or activity that is
integrally related to the development of abnormalities in lung vascular function and
structure in HPAH. Patient-specific blood-outgrowth endothelial cells (BOECs) expanded
ex vivo from peripheral blood mononuclear cells from patients with HPAH and healthy
subjects were used to examine the consequences of BMPR2 mutations on the BOEC
protein expression profile as well as on their functionality. Functional analyses of the
BOECs revealed that HPAH-derived BOECs are more susceptible to apoptosis and
more proliferative compared with healthy controls. Protein isolates of BOECs from
patients with HPAH and from healthy subjects were subjected to 2-D gel electrophoresis
and stained for total proteins and phosphoproteins, and to a quantitative computerassisted
analysis. Differentially regulated proteins were identified by mass spectrometry
(LC-MS/MS). Of the 416 total proteins detected under basal conditions, 11 were
significantly downregulated in HPAH-derived BOECs and 11, including the translationally
controlled tumor protein (TCTP), were upregulated. TCTP has previously been shown to
be involved in systemic arteriolar remodeling, inflammation and growth. Therefore, the
potential role of TCTP in PAH was studied in vivo in the SU5416 rat model of severe
angioproliferative PAH. Immunofluorescence staining revealed high expression of TCTP
in arteriolar ECs of PAH lungs tightly localized to proliferating cells within occlusive
intimal lesions; whereas, only minimal TCTP expression was seen in vascular ECs of normal lungs. Similarly, abundant TCTP immunostaining was also seen in human PAH
lung sections, again associated with complex vascular lesions. In BOECs, TCTP was
found to participate in cell growth and survival. These data suggest that TCTP could play
an important role in PAH by mediating pro-survival and growth signaling in vascular cells,
contributing to occlusive pulmonary vascular remodeling triggered by EC apoptosis.
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Translationally Controlled Tumour Protein as a Novel Therapeutic Target in Pulmonary Arterial HypertensionFoster, William Swinburne January 2016 (has links)
Background: Pulmonary arterial hypertension (PAH) is a multifaceted disease characterized by elevated pulmonary arterial pressure, right ventricular hypertrophy, and a poor prognosis. Pathological hallmarks of PAH include pulmonary vascular remodelling, pre-capillary arterial obliteration, and plexiform lesions. Over the past 15 years, pulmonary endothelial cell (EC) apoptosis has been repeatedly implicated as a key trigger of occlusive arterial remodelling in PAH. While it has been hypothesized that pulmonary EC apoptosis gives rise to the emergence of growth-dysregulated, apoptosis- resistant ECs involved in arterial remodelling, the molecular mechanisms linking these two events has not yet been fully elucidated. Recently, our lab identified translationally controlled tumour protein (TCTP) as one of several significantly dysregulated proteins in culture-derived blood-outgrowth endothelial cells (BOECs) isolated from hereditable PAH (HPAH) patients harbouring mutations in the gene encoding for bone morphogenetic protein receptor type 2. Immunohistological analyses indicated that TCTP expression was associated with intra-luminal pulmonary ECs and inflammatory cells in the remodelled vessels of both human PAH patients and SU5416 rats. Furthermore, TCTP silencing abrogated excessive HPAH BOEC proliferation and promoted apoptosis in vitro. Hypothesis: We hypothesized that TCTP represents a central molecular mechanism linking pulmonary arterial EC damage and apoptosis to the emergence of growth- dysregulated lung vascular cells and complex arterial remodelling in PAH.Purpose: The purpose of the present thesis was to examine the effects TCTP inhibition on EC survival and TCTP abundance in vitro as well as on pulmonary hemodynamic changes and arterial remodelling in vivo using a well-validated rat model of severe PAH. Methods: Inhibition of TCTP was accomplished using two TCTP small molecule inhibitors, sertraline and thioridazine. In vitro, rat lung microvascular ECs (RLMVECs) were exposed to thioridazine and assayed for TCTP abundance, survival, and markers of apoptosis. In vivo, PAH was induced in male Sprague Dawley rats using SU5416 combined with 3 weeks of chronic hypoxia (SU/CH). After 4 weeks, right ventricle systolic pressure (RVSP) was measured by direct catheterization and osmotic pumps containing either thioridazine or sertraline were implanted subcutaneously. Following 3 weeks of small molecule delivery, RVSP was re-evaluated, cardiac function/structure was determined using transthoracic echocardiography, and histological analyses of vascular remodelling and inflammation were performed. Results: Our in vitro experiments demonstrated that thioridazine was able to significantly down-regulate TCTP levels and induce an apoptotic phenotype in RLMVECs. In the SU/CH rat model of severe PAH, both thioridazine and sertraline failed to have any effect on pulmonary hemodynamics, right ventricle structure/function, or vascular remodelling. Moreover, neither small molecule was able to detectably down-regulate TCTP levels in the lungs of SU/CH rats. Immunofluorescence staining revealed that TCTP expression occasionally corresponded with the expression of macrophage/monocyte marker CD68 in the lungs of SU/CH rats, consistent with its expression by inflammatory cells; however, no significant differences were found in adventitial cell clearance in the presence or absence of the inhibitors. Conclusions: Our findings support previous reports that thioridazine is able to significantly down-regulate TCTP and induce apoptosis in vitro. In contrast, both small molecule inhibitors failed to down-regulate lung TCTP levels or have any beneficial effects on the progression of PAH in SU/CH rats.
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The Role of MicroRNAs in Endothelial Progenitor Cell FunctionBehbahani, John January 2016 (has links)
Cultures of peripheral blood mononuclear cells (MNCs) give rise to at least two different variants of endothelial progenitor cells (EPCs), early and late outgrowth EPCs. We investigated whether microRNAs in early and late EPCs could serve as markers of internal processes that can be exploited to distinguish cell identity and functional capacity. We hypothesized that as MNCs give rise to early and late EPCs, there is a gradual change in total microRNA profile, reflecting a total change in processes within the predominant cell population. Using a candidate microRNA array, early and late EPCs showed vastly different microRNA expression profiles. MiR-146a expression increased progressively as early EPCs emerged around 5-7 days (p<0.05). Through targeting TRAF6 and IRAK1, miR-146a conferred inflammatory tolerance in early EPCs, likely contributing to their purported ability to suppress inflammation. MiR-146a knock down (KD) in endotoxin-stimulated early EPCs reduced anti-inflammatory cytokine IL-1RA (p<0.001), and increased expression of pro-inflammatory cytokines IL-1 (p<0.001) and IL-8 (p<0.01). Interestingly, the microRNA expression profile of late EPCs was highly congruent to mature endothelial cells, with 100-fold greater miR-126 expression than monocytes and early EPCs (p<0.01). MiR-126KD in late EPCs abolished matrigel-network formation (p<0.05); while overexpression (OE) in early EPC augmented network formation (p<0.05) and chemotactic migration (p<0.001). We also found that the melanoma cell adhesion molecule or MCAM (CD146) identified late EPC precursors. Only MCAM+MNCs from adult blood (<5% of total MNCs) yielded late EPC-like colonies. Robust miR-126 expression in these cells predicted the generation of late EPCs. Overall, our results suggest that miR-146a in early EPCs likely contributes to repair by suppressing inflammation during cardiovascular injury; while in late EPCs, miR-126 directly promotes angiogenesis and vascular repair. Finally, we highlight a unique method for the efficient generation of late EPCs by using MCAM selection and screening for miR-126.
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Effects of Extracellular Matrix Glycation on Cell and Tissue FunctionNadlacki, Borivoje Bora January 2017 (has links)
Methylglyoxal (MG) is a reactive dicarbonyl derived as a by-product of glycolysis. If MG is not metabolized by glyoxalase-1 (Glo1), it glycates macromolecules producing advanced glycation end products (AGEs); these have been linked to larger infarct sizes and poorer cardiac function after myocardial infarction (MI). Proteins of the extracellular matrix (ECM) are prime targets for glycation by MG, but it is unknown if MG modification of the ECM may be a mechanism that contributes to the poor repair and function of the post-MI heart. This study sought to examine if MG-induced modifications of ECM proteins negatively affect fibroblast and endothelial cell function. Analysis with an MG-derived hydroimidazolone 1 (MG-H1) antibody confirmed MG modification of laminin and collagen type (Col) 1, 3, and 4. MG modifications decreased endothelial cell (EC) adhesion on Col3, Col4, and laminin and angiogenesis on ECMatrix. Furthermore, alpha smooth muscle actin staining indicated increased myofibroblast differentiation of fibroblasts on MG-modified proteins. Following induction of MI, extracted mouse hearts were decellularized and compared to healthy controls. Perhaps a result of technical challenges, both western blot and immunohistochemistry contrasted previous data by displaying a marked decrease in MG-H1 modifications post-MI. Overall, these results indicate that MG modifications of the ECM negatively influence EC and fibroblast function, requiring more research on their impact in cardiovascular disease progression.
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The Effects of High Glucose Exposure on Endothelial MicroparticlesTurner, Maddison January 2017 (has links)
Individuals with diabetes have an increased mortality due to the macro- and microvascular complications, which are commonly preceded by endothelial dysfunction. We have shown that endothelial microparticles (eMPs) are markers and mediators of vascular injury and pathology. However, their utility as a biomarker of hyperglycemia-induced endothelial damage and their influence on the vasculature remains unclear. We hypothesized that high glucose (HG) exposure alters eMPs protein composition, making them reflective of active signalling processes characteristic of a hyperglycemic environment. In addition, HG alters eMPs bioactivity, making them more potent inducers of oxidative stress, thrombosis and endothelial damage. Therefore, we assessed the exclusive effects of HG on eMPs formation, composition, and signalling. Results: Exposure of endothelial cells to high glucose for 24 hours caused a 3-fold increase in eMPs formation, increased mean vesicle size and their absolute electronegativity. Proteomic analysis of eMPs identified 1,212 independent proteins, with 68 exclusive to HG and associated with signalling processes related to metabolic processes, oxidation-reduction reactions, hemostasis and thrombosis and cellular interactions at the vascular wall. Compared to eMPs formed under normal conditions, eMPs formed in response to HG possess a ~3-fold greater procoagulant activity, induced a greater production of cellular ROS and were more potent inhibitors of endothelial-dependent relaxation. Conclusions/Interpretation: Taken together our results indicate HG alters the composition of eMPs, making them more potent mediators of endothelial damage. With similar changes in bioactivity being evident in the protein composition and the associated enriched biological processes, eMPs protein content may provide insight into the pathophysiological status of the cells in a hyperglycemic environment and provide use clinically, to identify dysregulated pathways for therapeutic targeting.
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Vitamin D and endothelial function and repair in Systemic Lupus ErythematosusReynolds, John January 2014 (has links)
Introduction: Patients with Systemic Lupus Erythematosus (SLE) have increased cardiovascular risk, endothelial dysfunction, and abnormal endothelial repair mechanisms. Vitamin D deficiency is common in SLE and has been associated with active disease and increased vascular stiffness. Myeloid angiogenic cells (MACs) repair damaged vessels by secretion of angiogenic factors and may be a target for vitamin D. Vitamin D may therefore be a novel therapy to improve cardiovascular risk in SLE patients. This study aimed to determine the effects of vitamin D on endothelial function and repair in patients with SLE. Methods: The effects of the active form of vitamin D (1,25(OH)2D3) were studied on MACs from vitamin D deficient SLE patients ex vivo. Functional models were developed to study MAC migration, adhesion and interaction with endothelial cells. Additional experiments used a model of healthy MACs treated with IFN-alpha. An observational study of clinically stable vitamin D deficient SLE patients being treated with high dose vitamin D over 3 months was used to investigate the effects of vitamin D on endothelial function as measured by flow-mediated dilatation (FMD). Results: MACs expressed markers consistent with an M2 macrophage phenotype and they enhanced endothelial network formation in vitro. SLE patients had an increased number of MACs; however, these were dysfunctional compared to healthy controls. Vitamin D increased the number, changed the phenotype and improved the functional capacity of SLE MACs ex vivo. In addition, the angiogenic capacity of SLE MACs was restored toward that of healthy controls via a reduction in the anti-angiogenic cytokine IP10. Vitamin D-treated MACs were also more able to protect endothelial cells against TNF-mediated down-regulation of endothelial nitric oxide synthase (eNOS). In SLE patients treated with vitamin D, there was a strong correlation between the change in serum 25(OH)D and the change in the ratio of endothelium-dependent:independent dilatation (r=-0.650, p=0.006). This was accompanied by an increase in the number of MACs at 3 months (p=0.015). These observations were independent of changes in serum PTH, calcium or lupus disease activity. Conclusions: Vitamin D can target MACs and therefore offers a novel approach to improve endothelial repair in patients with SLE. In addition, vitamin D treatment in lupus patients resulted in an improvement in endothelial function, related to the change in vitamin D status. These results suggest that vitamin D could improve surrogate markers of cardiovascular disease and thus reduce cardiovascular risk in this patient group.
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HEPES Buffer Perfusate Alters Rabbit Lung Endothelial PermeabilityDouglas, G. C., Swanson, J. A., Kern, D. F. 01 January 1993 (has links)
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) has been shown to cause changes in cultured endothelial cells and smooth muscle function at concentrations from 5 to 25 mM. To determine whether HEPES also affects vascular permeability, the effects of two buffers, HEPES and phosphate, were compared in isolated perfused rabbit lungs. Hemodynamic parameters and vascular protein permeability-surface area products (PS) were measured after perfusion with the buffers. Endothelial permeability was measured for an anionic and a cationic albumin to assess the charge effects of the zwitterion buffer. With HEPES, there were no changes in vascular pressure or resistance but permeability was affected. Cationic albumin permeability increased with 12 mM HEPES (8.7(phosphate) → 30(12 mM HEPES) x ml · min-1 · g dry lung-1 x 10-2) as did the anionic albumin PS (2.7(phosphate) → 3.52(12 mM HEPES). The cationic PS returned to baseline (8.1(60 mM HEPES)) at 60 mM HEPES, but the anionic PS did not change from the 12 mM HEPES (4.01(60 mM HEPES)). In summary, we find that HEPES is not innocuous. Although hemodynamic parameters did not change, endothelial permeability was increased when HEPES was used at normal concentrations. Therefore, HEPES should be used with caution as a physiological buffer in perfused organ systems.
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