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Roles of tannase and hydrolyzable tannins in chestnut blightFarias, Graciela Maria 19 June 2006 (has links)
<u>Endothia parasitica</u> (Murr.) P. J. & H. E. Anderson (syn: <u>Cryphonectria parasitica</u>(Murr.) Barr), the causal agent of chestnut blight, was able to grow in total aqueous and tannin extracts from blight-susceptible American chestnut as well as in blight-resistant Chinese chestnut bark extracts, from winter and summer bark. Differences in the amount of conidial germination and growth in extracts of the two species were small. The, <u>E. parasitica</u> tannase was more abundant intracellularly than extracellularly. Total tannase activities from cultures in American chestnut aqueous and tannin extracts were greater than in the Chinese chestnut extracts, for both winter and summer bark.
The tannase was isolated from the mycelium of E. parasitica and purified 142-fold with a 10% yield by anion exchange chromatography and gel filtration. The estimated molecular weight was 240 kD and the molecule may be a tetramer composed of four subunits with a molecular weight of 58 kD. The pH optimum of the purified tannase was 5.5 and the temperature optimum for activity was 30 C. The enzyme was separated into six bands in the pH range of 4.6 to 5.1 which may represent isoenzymes or post-translational modifications. / Ph. D.
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Biology of virulent and hypovirulent Endothia parasitica on American chestnut (Castanea dentata)Hebard, Frederick Vanuxem January 1982 (has links)
Results of inoculations of grafts, seedlings, excised stems, and intact trees of large, surviving American chestnut indicated that some have heritable blight resistance. Some degree of resistance in American chestnut possibly would assist spread of hypovirulence (H) factors. The pathogenicity of the H isolate but not the V (virulent) isolate appeared to influence the size and sporulating characteristics of biocontrolled H+V cankers.
The histopathology of canker development on blight-resistant Chinese and blight-resistant and blight-susceptible American chestnut was examined after inoculation with V and H E-parasitica. Rapid formation and continual growth of mycelial fans distinguished chestnut blight cankers incited by V E-parasitica from those incited by H E-parasitica, and distinguished cankers on susceptible chestnut from cankers on resistant chestnut. Differences among such cankers in wound periderm and other pathological alterations of bark and wood anatomy probably were results of differences in rates of fan formation and growth. Wound periderm formation began at the deepest point of a wound or canker and progressed outward to the bark surface. Superficial cankers arose in resistant trees or with hypovirulent fungus when slow-forming mycelial fans expanded through areas where wound periderm had not formed.
Rapid epidemics of chestnut blight occur in recently clearcut areas but not in areas with large trees of species other than American chestnut, most probably because chestnut sprouts released after cutting become larger than they do when overtopped by large forest trees. The size of chestnut sprouts appeared to influence the rate of blight progress by setting a maximum limit on canker size and thus canker sporulation. It may be possible to foster spread of H factors in the Appalachians if blight epidemics in clearcuts can be prolonged. This might be done by restricting inoculum production. Cutting and removing some blighted sprouts, cutting some large unblighted sprouts, or increasing blight resistance in the chestnut population are three potential means of restricting inoculum production. / Ph. D.
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