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Utveckling av enzymatisk bioremediering av PET : Användnin av ett kontextbaserat lärande i implementering av ett miljöperspektiv i gymnasieskolan / The development of enzymatic bioremediation of PET : The use of context-based learning in implementing an environmental perspective in secondary education.Jakobsson, Jessika January 2021 (has links)
Plastic pollution is one of if not the biggest threat against earth’s ecosystems. Almost 400 million tons of plastic is produced every year and most of it is discarded outside of the recycling systems. Marine ecosystems are extra exposed due to microplastics which are plastic smaller than 5 mm. The most common type of plastic is PET. Plastic in general is very chemically stable and hard to degrade but scientists have found a bacterium named I.sakaiensis that can degrade PET with a two-enzyme system called PETase and MHETase. Due to being exo-enzymes, they have to be secreted to function, the thermostability of these enzymes are very low so most research has been focused on increasing the thermal stability with its enzyme activity. This report focuses on what structures are important for the PET degrading ability of MHETas and PETas and how they can be applied to cleaning marine ecosystems. A key to solving environmental issues is creating environmentally aware students through the education system. Studies about Context based education have indicated that it sparks motivation and interest in students and the lessons seem more relevant. This report is also about how context-based education can be used to create an environmental perspective in secondary education.
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