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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Produção de enzimas coagulantes do leite por Aspergillus flavo furcatis em resíduos da agroindústria

Alecrim, Mircella Marialva 14 January 2014 (has links)
Submitted by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2016-03-03T13:17:31Z No. of bitstreams: 1 Dissertação - Mircella M. Alecrim.pdf: 558984 bytes, checksum: 9b8c2065b48d94ad76265bdf82a34748 (MD5) / Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2016-03-03T13:17:46Z (GMT) No. of bitstreams: 1 Dissertação - Mircella M. Alecrim.pdf: 558984 bytes, checksum: 9b8c2065b48d94ad76265bdf82a34748 (MD5) / Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2016-03-03T13:18:00Z (GMT) No. of bitstreams: 1 Dissertação - Mircella M. Alecrim.pdf: 558984 bytes, checksum: 9b8c2065b48d94ad76265bdf82a34748 (MD5) / Made available in DSpace on 2016-03-03T13:18:00Z (GMT). No. of bitstreams: 1 Dissertação - Mircella M. Alecrim.pdf: 558984 bytes, checksum: 9b8c2065b48d94ad76265bdf82a34748 (MD5) Previous issue date: 2014-01-14 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Six strains of Aspergillus flavo furcatis were screened to investigate milk-clotting enzyme production by fermentation in natural liquid medium. The growth media comprised extracts of Cupuaçu Exocarp+Rice Bran [10% or 20% (v/v) CE+RB] and Açai Waste+Rice Bran [10% or 20% (v/v) AW+RB] with or without supplementation 0.1% (w/v) of yeast extract and 0.5% (w/v) gelatin. Significant values of milk-clotting activity were determined by A. flavo furcatis DPUA 1461 and DPUA 1608, in the standard and natural media, respectively. According to criteria of clot and whey formations, 8.3% of the samples tested were classified as strong coagulation, 41.70% showed weak coagulation and in 50% wasn’t observed milk coagulation. Milk-clotting proteases of A. flavo furcatis DPUA 1608 were inhibited by pepstatin (94.72%) and moderately inhibited by the others metal ions tested. The optimal action was at 40 °C and pH 7.0. / Seis linhagens de Aspergillus flavo furcatis foram selecionadas para investigar a produção de proteases coagulantes por fermentação em meio líquido natural. O meio de cultura consistiu de extratos de Casca de Cupuaçu+Farelo de Arroz [CC+FA 10% ou 20% (v/v)] e Resíduo de Açaí+Farelo de Arroz [RA+FA 10% ou 20% (v/v)] com ou sem suplementação de extrato de levedura 0,1% (p/v) e gelatina 0,5% (p/v). Valores significativos de atividade coagulante foram determinados por A. flavo furcatis DPUA 1461 e DPUA 1608 no meio padrão e natural, respectivamente. De acordo com o critério de formação de coágulo e separação de soro, 8,3% das amostras testadas foram classificadas como coagulação forte, 41,70% apresentaram coagulação fraca e em 50% não foi observada a coagulação do leite. As proteases coagulantes de A. flavo furcatis DPUA 1608 foram inibidas por pepstatina (94,72%) e moderadamente inibidas por outros íons metálicos testados. A ação ótima das enzimas foi determinada a 40 °C e pH 7,0

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