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Development and application of polyclonal and monoclonal antibody based enzyme-linked immunosorbent assays for the analysis of neonicotinoid insecticides imidacloprid and thiamethoxamKim, Hee Joo. January 2003 (has links)
Thesis (Ph. D.)--University of Hawaii at Manoa, 2003. / Includes bibliographical references.
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Development and application of polyclonal and monoclonal antibody based enzyme-linked immunosorbent assays for the analysis of neonicotinoid insecticides imidacloprid and thiamethoxamKim, Hee Joo. January 2003 (has links)
Thesis (Ph. D.)--University of Hawaii at Manoa, 2003. / Includes bibliographical references. Also available by subscription via World Wide Web.
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Development of ELISAs for the detection of interferon-gamma in rhinoceroses and elephants as diagnostic tools for Mycobacterium bovis and Mycobacterium tuberculosis infectionsMorar, Darshana. January 2009 (has links)
Thesis (PhD (Veterinary Tropical Diseases, Veterinary Science))--University of Pretoria, 2009.
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Entwicklung eines indirekten ELISA zum serologischen Nachweis von Opisthorchis felineus (Rivolta, 1884) und Metorchis bilis (Braun, 1790) (Trematoda: Opisthorchiidae) beim Fuchs : mit einem Beitrag zu den Leberenzymaktivitäten bei experimenteller Opisthorchiidose /Dell, Kerstin. January 2001 (has links)
Berlin, Freie Universität, Thesis (doctoral), 2001.
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Validation and application of the ELISA technique for the detection of fish aero-antigens /George, Dashwill Anton. January 1900 (has links)
Thesis (MTech (Biomedical Technology))--Peninsula Technikon, 2003. / Word processed copy. Summary in English. Includes bibliographical references. Also available online.
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A new immunoassay for quantification of a novel cancer antigen in serum and immunostaining of carcinoma tissues and cultured cells revealing the antigenic cellular location /McDuffee, Emily. January 2002 (has links) (PDF)
Thesis (Ph. D.)--East Tennessee State University, 2002. / Includes bibliographical references. Also available via Internet at the UMI web site.
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Methodische und klinische Evaluierung eines ELISA-Testes zur Bestimmung des Annexin V als Marker in der Frühdiagnostik myokardialer Ischämien /Brunner, Karin. January 2002 (has links)
Giessen, Universiẗat, Thesis (doctoral), 2002.
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Development of an oral fluid assay for detection of uncontrolled diabetics using glycated albumin as a marker /Gelormo, David J., January 2002 (has links)
Thesis (Ph. D.)--Lehigh University, 2003. / Includes vita. Includes bibliographical references (leaves 150-165).
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Direct detection of mycobacterium tuberculosis in clinical specimens by PCR-ELISA王玲娜, Wang, Ling-na. January 2001 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
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Development and application of an ELISA method of analysis for fumonisinsBiden, Patricia May January 2000 (has links)
Fumonisins, mycotoxins produced by the fungus, Fusarium moniliforme, which grows on maize, are a major worldwide agricultural problem. Consumption of contaminated maize feeds causes a wide variety of toxic effects in animals depending on the species of animal. In
humans, high concentrations of fumonisins have been shown to correlate with increased incidence of oesophageal cancer (OC). Most analyses for fumonisins are done using high performance liquid chromatography (HPLC) which requires time-consuming extraction and clean-up prior to preparation of a fluorescent derivative. Enzyme-linked immunosorbent assays (ELISA), which are sensitive and specific, are a viable alternative but commercially
available antibodies and kits are extremely expensive.
Polyclonal antibodies against fumonisin B, (FB,) were raised in chickens and rabbits; all animals produced antibodies from week 2 onwards, the highest titre was at week 8 from one of the chickens. Cross-reactivities with FB, analogues were checked. A sensitive, quantitative competitive indirect ELISA (CI-ELISA) was developed and optimised; range 0.2 to 20 ng/ml
(in buffer), detection limit 0.2 nglml (in buffer), intra-assay coefficient of variation (CV) was 5.33 % and inter-assay 7.04%.
This method was adapted to analyse human plasma and urine samples. After removal of proteins by boiling, the range of recoveries of FBI were 94.7% toI12.4% at 4 ng/ml; and 94.6% to 108.7% at 8 ng/ml. Blood and urine samples from patients with OC (40 plasma, 17 urine), controls (21 plasma, 12 urine) and patients with other forms of cancer (20 plasma, 10
urine) were collected from hospitals in the Durban Metropolitan area and analysed for fumonisins. Detectable levels (>0.4 nglml) were found in 86.9% of plasma samples and 94.9% of urine samples. Statistical evaluation showed a highly significant difference between
plasma results for OC and controls (p<0.000 1) but no significant difference between the urine results. Comparison of other forms of cancer and controls showed no significant differences for either the plasma or the urine samples. However, there was a highly significant difference
between the OC and other forms of cancer results for both plasma (p<0.005) and urine (p<0.05) samples. Some samples (9 plasma, 8 urine) were checked by HPLC. For plasma samples there was correlation between the ELISA and HPLC methods (r = 0.656, p<0.005) but not for urine samples.
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