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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.

Application of biocatalysts in the resolution of peptidomimetic compounds from multi-component reactions

Kola, Fatima January 2017 (has links)
A dissertation submitted to the Faculty of Science University of the Witwatersrand Johannesburg in fulfilment of the requirements for the Degree of Master of Science. May 2017. / A library of potentially bioactive peptidomimetic compounds has been created using the Ugi and Passerini multi-component reactions. The products were isolated as racemic mixtures which we aimed to separate by enzymatic means. The Ugi enantiomers were obtained in exceedingly low yields due to considerable byproduct formation encountered with the use of ammonia as reactant and methanol as solvent. A threefold increase in the yields was achieved when employing the less nucleophilic 2,2,2-trifluoroethanol as solvent. An endeavour to resolve the racemates using proteases and penicillin G acylase proved unsuccessful, possibly attributable to the poor solubility of the Ugi products in a broad range of organic solvents. Enantiomers obtained from the Passerini reaction were efficaciously hydrolysed by lipase enzymes. Hence, the enantioselectivity of the hydrolysis reactions was explored by the employment of chiral HPLC and Mosher ester analysis. The biocatalysts displayed adequate selectivity for one of the adducts, making prospective separation of the racemic mixture viable. An investigation into the diastereoselectivity of the Passerini reaction was also performed. The application of phenylalanine-derived chiral starting materials in the reaction was seen to have a slight effect on reaction diastereoselectivity, giving on average a 2:1 diastereomeric ratio. Slightly greater d.r. values were obtained when bulkier isocyanides were employed. The diastereomeric mixtures were effectively separated via preparative HPLC to obtain pure compounds. 13C NMR spectroscopy indicated a trend in the chemical shift values for the newly formed stereogenic carbon centres for most of the two separated diastereomers. The C-2 values of most of the minor diastereomers appeared more downfield in comparison to the major diastereomers. Distinguishing the stereochemistry of the major and minor diastereomers through X-ray crystallography was not possible as the compounds were non-crystalline. Thus the configurations of the newly formed stereogenic centres of the major compounds were assigned by analogy with similar compounds previously identified in our laboratory. The major compounds were described as R at the newly formed stereogenic centre when L-phenylalaninal was used, and the minor products S. When D-phenylalaninal was used, the major diastereomer was assigned an S configuration at the newly formed stereocentre and the minor one was assigned R. The formation of the major diastereomer could be explained using a Felkin-Anh chelation controlled model, which has been observed in other reactions of mono protected amino aldehydes. / XL2018

The Role of the Quaternary Structure of a Bifunctional Enzyme

Findlay, Wendy A. 07 1900 (has links)

Development of rapid high capacity methods for the isolation of human N-acetyl beta-D-hexosaminidases

Hardwick, Jane January 1978 (has links)
No description available.

Les ferments alcooliques

Engel, Louis-Charles. January 1900 (has links)
Thèse de doctorat : Sciences naturelles : Paris, Faculté des sciences : 1872. / Titre provenant de l'écran-titre.

Development of chemically modified fluorescent beads to probe enzyme activity in microorganisms

Armstrong, Melinda Ann 05 1900 (has links)
No description available.

Development of rapid high capacity methods for the isolation of human N-acetyl beta-D-hexosaminidases

Hardwick, Jane January 1978 (has links)
No description available.

Studies on mechanisms involved in the induction of microsomal benzpyrene hydroxylase following pretreatment with 3,4-benzpyrene anddaflatoxin BO.

Gurtoo, Hira Lal, January 1968 (has links)
Thesis (Ph. D.)--Virginia Polytechnic Institute, 1968. / Also available via the Internet.

Structural basis of allosteric and intrasteric regulation in human cystathionine beta-synthase and its regulation by a CXXC motif

Sen, Suvajit. January 1900 (has links)
Thesis (Ph.D.)--University of Nebraska-Lincoln, 2007. / Title from title screen (site viewed Dec. 5, 2007). PDF text: 117 p. : ill. ; 6 Mb. UMI publication number: AAT 3271912. Includes bibliographical references. Also available in microfilm and microfiche formats.

Kinetic studies on propionyl-CoA carboxylase from pig heart /

Edwards, John Brian. January 1967 (has links) (PDF)
Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 1967. / "October 1967." Includes bibliographical references.

Production of commercially suitable pectin methylesterase and polyphenol oxidase from agro-industrial wastes/

Şimşek, Şebnem. Yemenicioğlu, Ahmet January 2004 (has links)
Thesis(Master)--İzmir Institute of Technology,İzmir, 2004 / Includes bibliographical references (leaves. 68).

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