Thesis (Ph. D.)--University of Washington, 1990. / Vita. Includes bibliographical references (leaves [287]-307).

Thesis (Ph. D.)--University of Washington, 1990. / Vita. Includes bibliographical references (leaves [210]-222).

A new immobilization technique has been developed in our lab using a new inert support material. Immobilized enzymes by the new method have a number of salient features of high activity, low cost and large catalytic area in comparison with the traditional immobilized enzymes. This new immobilization technique has potential to be widely applied for other enzymes. / Another new application of the immobilization method is to produce low lactose milk using immobilized lactase. A large population of people, especially the Asian, has intolerance to milk because of the lack of lactase in intestines. Pre-treatment of milk with immobilized lactase effectively reduces the milk lactose to an acceptable level for those people. / Enzyme was introduced for industrial application as early as nineteenth century. Immobilized enzymes have been developed since early 1960s. In recent years, immobilized enzymes have expanded their applications in many fields, food industry in particular, thanks to their superiority over the soluble forms: multiple or repetitive use; easier separation from reaction system; continuous production process; longer half-life and predictable decay rates. / Glucoamylase was also immobilized by the method. The differences between soluble and immobilized enzyme were compared. The potential using the immobilized glucoamylase in production of glucose syrup in replacement of liquid glucoamylase in current industrial process was assessed. / In this thesis, I have established the optimal immobilization conditions for three food enzymes, glucose isomerase, glucoamylase and lactase. The parameters including temperature, pH, protein capacity, and the ratios of cross-linker and adsorption agent have been studied in details. The applications of the consequently produced immobilized enzymes were studied and compared with the current industrial processes. / The novel immobilized glucose isomerase was applied to convert corn glucose into High Fructose Corn Syrup (HFCS) containing higher concentration of fructose than the current HFCS and achieved much better productivity than the existing immobilized glucose isomerases. / Shen, Dong. / "August 2007." / Advisers: Jun Wang; Kwok Kueng Ho. / Source: Dissertation Abstracts International, Volume: 69-02, Section: B, page: 1000. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (p. 175-194). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract in English and Chinese. / School code: 1307.

Glucose isomerase (GI) catalyzes the conversion of D-glucose to D-fructose in vitro. It is one of the bulkiest commercial enzymes, essential for the mass production of high-fructose corn syrup (HFCS) and crystalline fructose. / In this study, the effects of nitrogen sources, carbon sources, expression vectors, host strains, bacterial (Vitreoscilla) hemoglobin, selective pressure, plasmid stability and fermentation process on the GI production were investigated. The results showed that E. coli could express cloned thermostable GI at high expression level. E. coli transformed with the recombinant plasmid P-lac-GI gave the best result in term of total GI production and expression level. Corn steep liquor could be used as a cheap alternative nitrogen source for what was in LB medium. The concentration of glucose affected the expression level of GI significantly. Replacement of the ampicillin resistance gene by kanamycin resistance gene improved the plasmid stability leading to high productivity of GI in fed-batch fermentation. A suicide system could further improve the plasmid stability resulting in a high productivity of GI. A feeding strategy for fed-batch fermentation with the optimized parameters was developed to result in the production of up to 3g/L recombinant GI, which constituted 50% of the total soluble proteins. The total yield was 5-fold higher than that from flask experiments and 7-fold higher than the highest ever recorded. The expression level was also 100% higher than it was in other reports. / Liu Zhaoming. / "August 2005." / Advisers: J. Wang; W. P. Fong. / Source: Dissertation Abstracts International, Volume: 67-07, Section: B, page: 3780. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (p. 129-154). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract in English and Chinese. / School code: 1307.

Glucose isomerase (GI) catalyzes the isomerization of glucose to fructose and consequently is one of the bulkiest industrial enzyme for the manufacture of high fructose corn syrup and crystalline fructose. The GI is used in industry mainly in the form of immobilized enzyme. / In this work, the immobilization of GI had been studied by several methods: ion exchange adsorption, covalent binding, alginate cells entrapment and cells cross-linking. Three kinds of carrier support (ion exchange resin, epoxy resin and amino resin) have been used in the immobilization of cells-free enzyme; the whole cells immobilization of GI by cross-linking agents polyethyleneimid and glutaraldehyde were critically examined. The results show that the cells cross-linking is the best method to prepare the immobilized GI products, as it is high in specific activity and thermostability, and low the cost. The method is likely to make significant contribution to the field of immobilization, its application has expanding rapidly in many walks of the society, including environment protection, food and pharmaceutical industries. / Jin, Caike. / "August 2005." / Adviser: Jun Wang. / Source: Dissertation Abstracts International, Volume: 67-07, Section: B, page: 3521. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (p. 125-152). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract in English and Chinese. / School code: 1307.

Thesis (Ph. D.)--University of Washington, 2003. / Vita. Includes bibliographical references (leaves 152-171).

Thesis (doctoral)--Lund University, 1997. / Added t.p. with thesis statement inserted.

Thesis (doctoral)--Lund University, 1997. / Added t.p. with thesis statement inserted.