Lake Eola is a land-locked lake located in downtown Orlando, Florida. Its surface area is approximately 27.0 acres (11.0 Ha) and water depth is 2 to 3 feet (0.6 to 0.9 meters) near the shore and 22 feet (6.7 meters) toward the center. Periodical water samples were collected from the lake and storm drains for various stormwater events and physiochemical parameters were analyzed to calculated loading rates from nutrients and heavy metals released to Lake Eola. Algal bioassay studies were performed to investigate stormwater impacts on productivity. Periodical water samples were collected from the lake mixed and filtered for limiting nutrient studies using various concentrations of N, P, and Fe. Unialgal species of Selenastrum, Chlorella and indigenous species were used and changes in chlorophyll "a" and biomass were measured. Results indicate that phosphorus or nitrogen can be limiting at some times of the year. However, the ratio of P:N can be ore important than actual concentration of phosphorus and nitrogen separately. Similar algal bioassays were performed on a mixture of stormwater, coagulated stormwater and lake water at different ratios.