Thesis--Wisconsin. / Vita. Includes bibliographical references (leaves 93-95).

AbstractA study was made to determine if the age of a herbarium specimen of Equisetum hiemale had any effect on the chromatographic patterns. Specimens of E. hiemale were analyzed employing descending paper chromatography and electrophoresis.The amino acid and phenolic patterns were of significant value in determining if the chromatographic patterns varied as the herbarium specimen aged. The fluorescent phenolic compounds of E. hiemale were analyzed by placing the chromatograms under long wave ultraviolet light. The free amino acids were detected by spraying the chromatogram. with ninhydrin.The chromatographic patterns showed that the age of the oldest herbarium specimen investigated had no effect on its patterns.Ball State UniversityMuncie, IN 47306