Diversity of ectotrophic mycorrhizal (EM) fungi on out-planted seedlings of two oak species (Quercus rubra and Q. prinus) was estimated at two sites in mature mixed forests in the southern Appalachian mountains. Late – stage fungi were well represented. Total richness was 73 types, with 42 types having a frequency of only one. Thelephoroid / tomentelloid, russuloid, and cortinarioid groups were the richest. Dominant fungi included a putative Tuber sp. and Craterellus sp., and Laccaria cf laccata. Diversity was lower at a high elevation chestnut oak dominated site compared to a lower mesic cove – hardwood forest site. There was little evidence for fungal specificity to red oak versus white oak seedlings. We also compared EM fungus distributions on root systems of oak seedlings from samples taken in mid-July and early-September. The majority of EM types occurred only in the mid- or late-summer samples respectively. Dramatic shifts in mycobiont dominance were observed in relation to sample date, including increases in Cortinarius spp. richness, decreases in Thelephoraceae richness, and the disappearance of Amanita spp. types in the late- compared to mid-summer samples. A multi-stage model of seasonal EM dynamics is proposed, with implications for the niche expansion of associated phytobionts. In this model, generalistic mycobionts are most frequent and occur throughout the season. Other more specialized fungi show seasonal specificity. Relationships between species and communities of EM fungi and environmental parameters such as ericoid shrub abundance and edaphic characteristics were also examined. High diversity of EM fungi limited resolution of community level relationships given our sample sizes. Intraspecific variation in EM fungi with regard to microsite characteristics was also undetectable. No association between ericoid shrub dominance (Kalmia latifolia and Rhododendron maximum) and EM fungi was observed. We present a listing of EM fungus types with associated ranges of edaphic parameters and ericoid shrub abundance. The family Sebacinaceae is a basal hymenomycete lineage that includes members of the genera Tremellodendron and Sebacina. We present evidence suggesting the putative mycorrhizal status of two species of Tremellodendron. Tremellodendron appears to form both endophytic associations with achlorophyllous orchids and ectomycorrhizae with species of Quercus, Pinus and Tilia cordata. / Ph. D.

The seasonal changes in freezing resistance and water relations in four dwarf shrub species of Ericaceae family: Vaccinium vitis-idaea, V. myrtillus, V. uliginosum and Oxycoccus palustris in two study areas differing in altitude were studied in this work. To establish the freezing resistance, the plants were sampled eight times, to determine parameters of the water relations, the plants were sampled six times during the growing season of 2009. For the freezing resistance examination, ice nucleation temperature (exotherm) and 50 per cent lethal temperature (LT50) were found. The water relations were described using the parameters derived from the pressure-volume curves: osmotic potential at full turgor, osmotic potential at zero turgor, cytoplasmic water fraction, relative water content at turgor loss and bulk modulus of elasticity. An important seasonal variability in the freezing resistance was found. The highest levels of the freezing resistance were reached in the spring and in the autumn, the lowest levels were found in the summer. There were also changes in the used mechanisms of the freezing resistance - for most of the year, the plants were tolerant, they showed the avoidance mechanism only in the summer. The seasonal changes in the water relation parameter were less unambiguous. The most of...