Eucalyptus grandis was introduced into South Africa in the 19th century, and has since become the most important of the hardwood plantation tree species grown for pulp. Until the late 1980's E. grandis was virtually the only eucalypt species grown. In order to meet the increasing demand for pulpwood in South Africa, forestry companies need to increase their timber output from an existing land base (Brown and Hillis, 1984; Kimmins, 1994 and Little and Gardner, 2003), or alternatively extend the planting of favourable alternative tree species into areas previously considered unsuitable for forestry due to unfavourable climatic conditions. From 1984 the major timber companies expanded their plantation forestry into the colder, frost-prone highland areas of western KwaZulu-Natal, the northeastern Cape and southeastern Mpumalanga Highveld. As E. grandis was not tolerant to severe frost, E. macarthurii amd E. nitens were planted in these areas as alternatives (Schonau and Gardner, 1991). As much of the earlier research had been centered around the development of silvicultural standards for E. grandis, it became necessary to test these for the different eucalypt species. Two trials were therefore established to E. marcarthurii and E. nitens with the following objectives: - to extend current recommendations to include different species, - to determine the degree of interaction between different silvicultural standards (genetic improvement, fertilisation and weed control), - to determine the effects of weeds, fertilisation, genotype and regeneration method (seedling vs coppicing) on the initial and long term growth, uniformity, tree straightness and survival of cold tolerant eucalypts, - cost effectiveness of various methods for re-establishing E. nitens and E. macarthurii. Genetic improvement played an important role in the establishment and initial growth of E. macarthurii and E. nitens. The improved treatments outperformed the unimproved treatments in terms of tree growth until canopy closure. At the last measured date when the trees were six years of age, the E. nitens improved seedlings were still significantly better in terms of basal area when compared to unimproved seedlings. The initial positive effect of genetic improvement of E. macarthurii seedling however, was not sustained. Genetic improvement of E. macarthurii and E. nitens also had a positive effect on tree straightness and survival when the trees were assessed at five years of age. The genetic improvement of both species also showed to be a viable option to produce an optimum timber output at a lower cost when regeneration is carried out by means of replanting with seedlings. Fertilisation also showed positive effects in terms of the establishment and initial growth of E. macarthurii and E. nitens. At six years after planting, the basal area of E. macarthurii seedlings without fertiliser was still significantly lower than any one of the other treatments. However, the initial positive effect fertiliser had on the growth of E. nitens seedlings decreased to a non-significant level at six years after planting. Fertilisation of E. macarthurii and E. nitens had a positive effect on tree straightness and survival when the trees were assessed at five years of age. The fertilisation of E. macarthurii seedlings also produced an adequate amount of timber at a relatively low cost. The controlling of weeds did not have an impact on tree performance initially or after canopy closure for either E. macarthurii or E. nitens. This is due to the lack of weed growth at these high altitudes at which the sites were planted. Little and Schumann (1996) found that eucalypts could tolerate an aboveground weed biomass of up to 2000 kg ha (-1) before there were any severe losses in growth due to competition. At both these trials, the weed load did not reach these levels in order to compete with the trees. No significant interactions between any of the treatments were detected at both these sites at any stage. At the last measured date, there were no significant differences in terms of tree growth between the coppice and seedling treatments for either E. macarthurii or E. nitens. Regeneration by means of E. macarthurii and E. nitens coppice had a positive effect on tree straightness and survival when the trees were assessed at five years of age. Re-establishment by means of coppice for both E. macarthurii and E. nitens was also shown to be by far the most cost-effective way at present to produce an adequate amount of timber. Coppicing was shown to be the least costly way to produce a m2 ha(-1) of timber provided the right species are coppiced, and optimum density levels are obtained. / Thesis (M.Sc.Agric.)-University of KwaZulu-Natal, Pietermaritzburg, 2005.

Eucalypt trees are one of tree species used for the manufacturing of papers in South Africa. The manufacturing of paper consists of cooking the wood with chemicals until obtaining a pulp. The wood is made of different cells. The shape and structure of these cells, called wood anatomical characteristics are important for the quality of paper. In addition, the anatomical characteristics of wood are influenced by environmental factors like climatic factors, soil compositions etc…. In this study we investigated the effects of the climatic factors (temperature, rainfall, solar radiation, relative humidity, and wind speed) on wood anatomical characteristics of two Eucalyptus clones, a GC (Eucalyptus grandis × camuldulensis) and a GU (Eucalyptus grandis × urophylla). Nine trees per clone have been selected. Two sets of data have been collected for this study. The first set of data was eleven anatomical characteristics of the wood formed daily over a period of five years. The second set of data was the daily measurement of temperature, rainfall, solar radiation, relative humidity and wind speed in the experimental area. Wood is made of two kinds of cell, the fibres and the vessels. The fibres are used for the strength and support of the tree and the vessels for the nutrition. Eleven characteristics related to those cells have been measured (diameter, wall thickness, frequency). These characteristics are highly correlated. To reduce the number of response variables, the principal component analysis was used and the first four principal components accounts for about 95% of the total variation. Based on the weights associated with each component the first four principal components were labelled as vessel dimension (VD), fibre dimension (FD), fibre wall (FW) and vessel frequency (VF). The longitudinal linear mixed model with age, season, temperature, rainfall, solar radiation, relative humidity and wind speed as the fixed effects factors and tree as random effect factor was fitted to the data. From time series modelling result, lagged order of climatic variables were identified and these lagged climatic variables were included in the model. To account for the physical characteristic of the trees we included the effect of diameter at breast height, stem radius, daily radial increment, and the suppression or dominance of the tree in the model. It was found that wood anatomical characteristics of the two clones were more affected by climatic variables when the tree was on juvenile stage as compared to mature stage. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

It is believed that the process-based model 3-PG (Physiological Principles Predicting Growth; Landsberg and Waring, 1997) can potentially play a useful role within South African forestry, both as an operational and a strategic tool. Strategic applications may include the prediction of potential productivity on a site-by-site basis; broadscale productivity estimates based on remote sensing and the spatial application of 3-PG; identification of production constraints; and estimation of carbon fluxes to help address sustainability issues. Operationally, 3-PG could complement empiricallybased models or be used in conjunction with them as a hybridised product. The challenges of this study were therefore to see whether it is possible to adapt 3-PG to predict the growth and yield of E. grandis under South African conditions, test that model predictions are consistent with observed growth data and are biologically reasonable, and to assess the practicality of using 3-PG as either a strategic or operational tool. The main emphasis of this study was to understand the internal logic of 3-PG and how physiological processes are represented, and to develop methods to objectively parameterise and initialise the model. Thereafter a detailed model validation study was performed, ending off with selected potential applications of 3-PG within the South African context. The sensitivity of predicted stand volume (SV) and leaf area index (LAI) to the values of the species-specific parameters in 3-PG was examined. These analyses enabled the development of three distinct parameter sensitivity classes: insensitive parameters (i.e. those that can be varied widely without affecting the outputs studied), sensitive parameters (i.e. those whose value strongly affects the outputs, and non-linear parameters (i.e. those for which the outputs depend in a non-linear manner on the parameter value). Minimum data requirements for the parameterisation and initialisation of 3-PG are considered in detail. Conventional methods used for the parameterisation of models, specifically 3-PG, are reflected upon. An automated parameter estimation technique was examined and used for the estimation of selected parameters. Species-specific parameters were categorised according to data source estimation and sensitivity classes. Parameters describing allometric and age-dependent relationships were assigned values using observed data from biomass harvests. Critical parameters that could not be directly assigned using observed data were the ratio of foliage to stem allocation (i.e. P2 and p2o), allocation of net primary production (NPP) to roots (TJRX and T]Rn), optimum temperature for growth (7^,) and maximum canopy quantum efficiency (acx)- These were estimated using Parameter ESTimation, by fitting model output to corresponding observed growth data. As well as species-specific parameter values, mandatory inputs required by 3-PG include weather data, site-specific factors such as site fertility (FR) and physical properties of the soils, and stand initialisation data. Objective techniques to determine these site-specific factors and the initial values for the biomass pools were proposed. Most of these data are readily available for sites where experimental trials exist, or where monitoring networks are in place. However, this is the exception rather than the rule, so alternative data and information sources are required. These, together with the need for accurate weather inputs (especially monthly rainfall) and physical properties (especially soil texture, maximum available soil water and FR) of the sites being modelled were explored. 3-PG was validated using four simple tests by comparing predicted versus observed SV. Results showed that 3-PG predictions are relatively consistent with observed stand data. Analyses performed using time-series data showed model predictions accurately tracked observed growth in response to erratic and fluctuating weather conditions. Results from the initial model validation showed production on high and low productivity sites was under- and over-predicted, respectively. Further results presented here show a similar, but less marked trend (i.e. over- and under-predictions are not as extreme), and individual biases are less than those from predictions made using another locally developed parameter set. The application of 3-PG showed that the model is able to make estimates of tree growth that are consistent with those used within the forestry site classification. This showed the considerable potential 3-PG has for strategic planning by the forest industry (i.e. projected wood supplies etc) and in research planning (refining existing site classifications). The model could be useful in predicting growth in various areas where E. grandis is not grown, assisting in future decision making. 3-PG was able to identify growth constraints on a site-by-site basis and distinguish among them, and was able to identify environmental and site limitations to plantation growth, and how they vary in space and time. These results together with predictions of site productivity demonstrate the potential for 3-PG to be used to improve existing forest site classifications. The model comparison study between empirically-based models and 3-PG showed that although the empirical models made accurate predictions of volume under static climatic conditions, under fluctuating weather conditions empirical estimates of volume were less accurate than those made with 3-PG. 3-PG can therefore be used operationally with minimum input data to predict growth using enumeration data. This is useful in saving time and cutting costs. The use of process-based models (PBMs) in general, and 3-PG in particular, needs to be "championed'' to the South African forest industry. This is necessary for two reasons. Firstly, the model and the manner with which it describes physiological processes of growth need to be explained in layman's terms. This will demonstrate how and why a process-based model can work better in a fluctuating environment than empirically based models. Secondly the comparison between 3-PG and the local empirical models needs to be presented as an example of how 3-PG can be applied on an operational basis. It is accepted that much convincing is still required. / Thesis (Ph.D.)-University of KwaZulu-Natal, Durban, 2005.

In order to determine if weed control as practised during the establishment phase of tree growth had a beneficial and long term (over a six to eight year rotation) impact on tree performance, a Eucalyptus hybrid clone (GC304) was planted in a field trial in 1990. The trial was situated in the coastal Zululand region near the KwaZulu-Natal town of Mtunzini. Nine different vegetation management treatments were imposed from establishment. These included a weedy control, a manually weeded treatment, a chemically weeded treatment, a 1.2 m row and 1.2 m inter-row weeding, a 0.5 m radius ring weeding, a complete weeding except for a 0.5 m radius ring around the tree, and the use of two legume cover-crops, Mucuna puriens (cowpea) and Vigna sinensis (velvet bean). Initial improvements in tree performance from these competition control treatments were detected from 60 days after planting, and were maintained over seven growing seasons. This occurred despite the absence of competitive vegetation after the first growing season due to reduced light, following crown canopy closure. There were strong indications that initial competition was mainly for moisture and possibly also for nutrients, rather than competition for light. Initially those trees that had weeds within their immediate vicinity were most affected (weedy control, inter-row weeding and the complete weeding except for a 0.5 m radius around the tree). With time, tree performance was more closely related to an increase in the percentage of the area kept free of weeds. The best performing treatment at felling, the manually weeded control, produced 17.1 % and 38.5 % more merchantable timber than the 1.2 m row weeding and the weedy control, at an increased profit of 8 % and 27 %, respectively. Two forms of competition (interspecific and intraspecific competition) were evident in the weedy control at different stages of tree development in contrast to the one (intraspecific competition) in the manually weeded treatment. Interspecific competition resulted in greater variability between the trees in the weedy control by the time canopy closure had occurred. This differentiation in tree size was further enhanced by asymmetric intraspecific competition once the trees had become established. The onset of intraspecific competition was first detected 995 days after planting for the manually weeded treatment and 1641 days after planting for the weedy control. Of the various competition indices that were tested in order to try and explain this differential growth in terms of individual tree performance, none was able to do so to complete satisfaction. The growth rates of different tree size classes were therefore compared for the weedy control and manually weeded treatment. The diverging slopes of the different stem area classes indicated that the larger trees were growing at the expense of the smaller trees. This type of competition is known as asymmetric intraspecific competition. In addition, a comparison was made between the slopes for the weedy and weedfree treatments for similar stem area classes. No significant difference was detected, indicating that similar size classes in these two treatments grew at similar rates. Trees from three treatments were selected (manually weeded treatment, 1.2 m row weeding treatment and the weedy control) and tested for the wood and pulping properties of density, active alkali consumption, extractable content, screened pulp yield, pulp yield per hectare and fibre length and coarseness. The use of Canonical Variate Analysis to determine if there were differences between the three treatments for the variates measured, indicated that they were significantly different. There was a significantly positive trend of an increase in density, extractable content and active alkali consumption with increased weed control. A possible explanation for this could be that the larger trees of the manually weeded treatment were under more stress (from increased intraspecific competition) during the latter phase of their growth. This was demonstrated by comparing the growth rates for these three treatments. The smaller trees of the 1.2 m row weeding treatment and the weedy control exhibited a lower rate of decline. As no significant difference was detected for screened pulp yield between the treatments, any differences in the pulp yield per hectare values could be attributed to differences in the merchantable volume. There was a 22.6 % and 40.8 % increase in the pulp yield per hectare for the manually weeded treatment in comparison to the 1.2 m row weeding treatment and the weedy control. The planting of cover-crops, although beneficial in terms of weed suppression, caused significant tree suppression. This occurred despite the fact that their initial biomass accumulation was slower than that of the natural weed population. Of the two cover-crops, the use of the velvet bean was not considered suitable due to its vigorous vining habit which affected the growth form of the trees. Subsequent work suggests that if the beneficial qualities of cowpeas are to be realised (that of weed suppression, erosion control and nitrogen fixation), a delay in their planting by three months after establishment of the trees should alleviate any negative impacts on tree growth. / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 1999.

A study was undertaken to compare growth potential of three Eucalyptus species of natural origin in South Eastern Australia, with commercially grown species on mid and high altitude temperate sites in the summer rainfall growing zones in South Africa. The three species were Eucalyptus globulus subsp. bicostata (E. bicostata), Eucalyptus cypellocarpa and Eucalyptus nobilis. Nine sites were selected to represent the growing areas of South Africa experiencing cold winter drought with occasional snow falls. Improved, commercially grown pure Eucalyptus species and interspecific hybrid clones were included as controls in the trials to give comparative growth performances on specific sites. Both balanced and unbalanced lattice designs were used to evaluate the growth potential of the unimproved Australian species and the improved commercial controls incorporated into the trials. Volume production and basal area growth were assessed for the three species at all sites. However, only three sub species E.globulus (E. bicostata, E. maidenii and E. globulus) showed varying levels of disease (Mychosphaerella nobilosa) infestation and this was therefore assessed in trials at 12 and 30 months respectively, and correlation analysis was used to study the relationship between the impact of disease infestation and growth performance. It was found that negative phenotypic correlations existed between the levels of infection and tree growth. ie greater infection slower growth. Evaluation of genotype x environment interaction (GXE) revealed that this did not exist in the E. bicostata nor E. nobilis populations, and therefore one population of each species can be developed for all the sites tested. E. cypellocarpa was the only species that showed some GXE interaction implying that separate populations to be developed for different sites. Contrary to what was expected, unimproved Eucalyptus species being investigated performed equally as well as the improved commercial species included as controls, thus providing potential for commercial deployment with selection and breeding. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2008.

To produce high quality paper, lignin should be removed from the pulp. Quantification of lignin concentrations using standard wet chemistry is accurate but time consuming and costly, thus not appropriate for a large number of samples. The ability of hyperspectral remote sensing to predict foliar lignin concentrations could be utilized to estimate wood lignin concentrations if meaningful relationships between wood and foliar chemistry are established. LAI (leaf area index) is a useful parameter that is incorporated into physiological models in forest assessment. Measuring LAI over vast areas is labour intensive and expensive; therefore, LAI has been correlated to vegetation indices using remote sensing. Broadband indices use average spectral information over broad bandwidths; therefore details on the characteristics of the forest canopy are compromised and averaged. Moreover, the broadband indices are known to be highly affected by soil background at low vegetation cover. The aim of this study is to determine foliar and wood lignin concentrations of Eucalyptus clones using hyperspectral lignin indices, and to estimate LAI of Eucalyptus clones from narrowband vegetation indices in Zululand, South Africa Twelve Eucalyptus compartments of ages between 6 and 9 years were selected and 5 trees were randomly sampled from each compartment. A Hyperion image was acquired within ten days of field sampling, SI and LAI measurements. Leaf samples were analyzed in the laboratory using the Klason method as per Tappi standards (Tappi, 1996-1997). Wood samples were analyzed for lignin concentrations using a NIRS (Near Infrared Spectroscopy) instrument. The results showed that there is no general model for predicting wood lignin concentrations from foliar lignin concentrations in Eucalyptus clones of ages between 6 and 9 years. Regression analysis performed for individual compartments and on compartments grouped according to age and SI showed that the relationship between wood and foliar lignin concentration is site and age specific. A Hyperion image was georeferenced and atmospherically corrected using ENVI FLAASH 4.2. The equation to calculate lignin indices for this study was: L1R= ~n5il: A'''''y . 1750 AI680 The relationship between the lignin index and laboratory-measured foliar lignin was significant with R2 = 0.79. This relationship was used to calculate imagepredicted foliar lignin concentrations. Firstly, the compartment specific equations were used to calculate predicted wood lignin concentrations from predicted foliar lignin concentrations. The relationship between the laboratorymeasured wood lignin concentrations and predicted wood lignin concentrations was significant with R2 = 0.91. Secondly, the age and site-specific equations were used to convert foliar lignin concentration to wood lignin concentrations. The wood lignin concentrations predicted from these equations were then compared to the laboratory-measured wood lignin concentrations using linear regression and the R2 was 0.79 with a p-value lower than 0.001. Two bands were used to calculate nine vegetation indices; one band from the near infrared (NIR) region and the other from the short wave infrared (SWIR). Correlations between the Vis and the LAI measurements were generated and . then evaluated to determine the most effective VI for estimating LAI of Eucalyptus plantations. All the results obtained were significant but the NU and MNU showed possible problems of saturation. The MNDVI*SR and SAVI*SR produced the most significant relationships with LAI with R2 values of 0.899 and 0.897 respectively. The standard error for both correlations was very low, at 0.080, and the p-value of 0.001. It was concluded that the Eucalyptus wood lignin concentrations can be predicted using hyperspectral remote sensing, hence wood and foliar lignin concentrations can be fairly accurately mapped across compartments. LAI significantly correlated to eight of the nine selected vegetation indices. Seven Vis are more suitable for LAI estimations in the Eucalyptus plantations in Zululand. The NU and MNU can only be used for LAI estimations in arid or semi-arid areas. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.