Chan Yiu-Pong. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 98-112). / Abstracts in English and Chinese. / Abstract --- p.i / Acknowledgement --- p.v / Declaration --- p.vi / Table of content --- p.vii / List of Tables --- p.x / List of Figures --- p.xi / List of abbreviation --- p.xiv / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Introduction of Semen Oroxyli --- p.1 / Chapter 1.1.1 --- Chemical constituents of Oroxylum indicum seed --- p.3 / Chapter 1.1.2 --- Pharmacological studies --- p.3 / Chapter 1.2 --- Introduction to tracheal secretion --- p.6 / Chapter 1.2.1 --- Mucus composition --- p.6 / Chapter 1.2.2 --- Sputum formation --- p.6 / Chapter 1.2.3 --- Expectorant --- p.7 / Chapter 1.2.3.1 --- Secretolytic drugs --- p.7 / Chapter 1.2.3.2 --- Mucolytic drugs --- p.8 / Chapter 1.2.4 --- Assays of studying expectorant activity --- p.10 / Chapter 1.2.4.1 --- Tracheal phenol red secretion system --- p.10 / Chapter 1.3 --- Introduction to oxidant and antioxidant --- p.11 / Chapter 1.3.1 --- Oxidants/ reactive oxygen species --- p.11 / Chapter 1.3.1.1 --- Production of oxidants/ reactive oxygen species --- p.11 / Chapter 1.3.1.2 --- Reactive oxygen species reaction products --- p.12 / Chapter 1.3.1.2.1 --- DNA damage --- p.13 / Chapter 1.3.1.2.2 --- Protein damage --- p.13 / Chapter 1.3.1.2.3 --- Lipid damage --- p.14 / Chapter 1.3.2 --- Antioxidant --- p.14 / Chapter 1.3.2.1 --- Endogenous antioxidants --- p.15 / Chapter 1.3.2.1.1 --- Superoxide dismutase --- p.15 / Chapter 1.3.2.1.2 --- Catalase --- p.15 / Chapter 1.3.2.1.3 --- Glutathione and glutathione peroxidases --- p.15 / Chapter 1.3.3 --- Synthetic and natural antioxidants --- p.16 / Chapter 1.3.3.1 --- Vitamin C --- p.17 / Chapter 1.3.3.2 --- Tocopherols (Vitamin E) --- p.17 / Chapter 1.3.4 --- Assays for studying antioxidative activities --- p.19 / Chapter 1.3.4.1 --- DPPH radical scavenging system --- p.19 / Chapter 1.3.4.2 --- PMS-NADH system --- p.19 / Chapter 1.3.4.3 --- APPH-induced hemolysis system --- p.20 / Chapter 1.4 --- Objectives of the research --- p.22 / Chapter Chapter 2 --- Materials and Methods --- p.24 / Chapter 2.1 --- Materials --- p.24 / Chapter 2.1.1 --- Semen Oroxyli --- p.24 / Chapter 2.1.2 --- Animals --- p.24 / Chapter 2.1.3 --- Chemicals --- p.25 / Chapter 2.2 --- Methods --- p.28 / Chapter 2.2.1 --- Assay for studying expectorant activity --- p.28 / Chapter 2.2.1.1 --- Phenol red standard curve --- p.28 / Chapter 2.2.1.2 --- Tracheal phenol red secretion system --- p.28 / Chapter 2.2.2 --- Assays for studying antioxidative activity --- p.30 / Chapter 2.2.2.1 --- DPPH radicals scavenging system --- p.30 / Chapter 2.2.2.2 --- PMS-NADH system --- p.31 / Chapter 2.2.2.3 --- AAPH-induced red blood cell hemolysis system --- p.32 / Chapter 2.2.3 --- "Extraction, fractionation and purification of Semen Oroxyli" --- p.33 / Chapter 2.2.3.1 --- 70% ethanol extraction of Semen Oroxyli --- p.33 / Chapter 2.2.3.2 --- Fractionation in polyamide column --- p.33 / Chapter 2.2.3.3 --- Fractionation in resin column --- p.34 / Chapter 2.2.3.4 --- Sub-fractions separation from 95% ethanol soluble fraction --- p.34 / Chapter 2.2.3.5 --- Pure compounds obtained from sub-fractions --- p.34 / Chapter 2.2.4 --- Nulcear magnetic resonance (NMR) for identification --- p.38 / Chapter Chapter 3 --- Results of Expectorant Activity --- p.39 / Chapter 3.1 --- Expectorant Activity --- p.39 / Chapter 3.1.1 --- Expectorant Activity on Semen Oroxyli ethanol extract --- p.39 / Chapter 3.1.2 --- Expectorant activities of fractionations of Semen Oroxyli ethanol extract --- p.39 / Chapter Chapter 4 --- Results of Antioxidative Activity --- p.44 / Chapter 4.1 --- Antioxidative activity --- p.44 / Chapter 4.1.1 --- Antioxidative activity of 70% ethanol extract --- p.44 / Chapter 4.1.2 --- Antioxidative activity of fractions of 70% ethanol extract --- p.49 / Chapter 4.1.3 --- Antioxidative activity on sub-fractions fractionated from 95% ethanol-soluble fraction --- p.50 / Chapter 4.1.4 --- Antioxidative activity on pure compounds isolated from sub-fractions --- p.59 / Chapter Chapter 5 --- Results of Identification of Pure compounds --- p.68 / Chapter 5.1 --- Identification of compounds --- p.68 / Chapter 5.1.1 --- Compound A --- p.68 / Chapter 5.1.2 --- Compound B --- p.69 / Chapter 5.1.3 --- Compound C --- p.69 / Chapter 5.1.4 --- Compound D --- p.70 / Chapter 5.1.5 --- Compound E --- p.71 / Chapter Chapter 6 --- Discussion --- p.73 / Chapter 6.1 --- Discussion of expectorant activity of Semen Oroxyli --- p.73 / Chapter 6.2 --- Discussion of antioxidative activity of Semen Oroxyli --- p.75 / Chapter 6.3 --- General Discussion --- p.77 / Chapter Chapter 7 --- Conclusions --- p.82 / Appendix A Procedures for preparing the phenol red standard curve for tracheal phenol red secretion system. --- p.85 / Appedix B 1H NMR and 13C NMR spectra --- p.87 / References --- p.98