by Sze, Lung Yam. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 106-112). / Abstract --- p.i / Acknowledgements --- p.iv / Contents --- p.v / Chapter Chapter 1 --- General Introduction / Chapter 1.1. --- Morphology of the Mammalian Somite and Limb --- p.1 / Chapter 1.1.1. --- The Somite --- p.1 / Chapter 1.1.2. --- The Limb --- p.3 / Chapter 1.2. --- Somite-Limb Relationship --- p.5 / Chapter 1.2.1. --- Somite Contribution to the Appendicular Musculature --- p.5 / Chapter 1.2.2. --- Somite Contribution to Limb Morphogenesis --- p.6 / Chapter 1.3. --- Control of Directionality of Somitic Cell Migration in Appendicular Environment --- p.8 / Chapter 1.4. --- Reasons and Objective of the Present Study --- p.10 / Chapter Chapter 2 --- The Origin of the Mammalian Limb Skeletal Muscles / Chapter 2.1. --- Introduction --- p.16 / Chapter 2.2. --- Materials and Methods --- p.19 / Chapter 2.2.1. --- Embryo Collection --- p.19 / Chapter 2.2.2. --- Isolation of Somites --- p.20 / Chapter 2.2.3. --- DiI-labelling of Rat Donor Somites --- p.21 / Chapter 2.2.4. --- Somite Transplantation --- p.21 / Chapter 2.2.5. --- Embryo Culture --- p.22 / Chapter 2.2.6. --- Analysis of Cultured Embryos --- p.22 / Chapter 2.2.7. --- Cryosection --- p.23 / Chapter 2.2.8. --- Limb Explant Cultures --- p.23 / Chapter 2.2.9. --- Immunohistochemistry --- p.24 / Chapter 2.2.10. --- X-gal Staining --- p.25 / Chapter 2.2.11. --- Histology --- p.25 / Chapter 2.3. --- Results --- p.27 / Chapter 2.3.1. --- Gross Morphology of Cultured Embryos --- p.27 / Chapter 2.3.2. --- Distribution of DiI Labelled Somitic Cells in Rat Embryos --- p.27 / Chapter 2.3.3. --- Histogenetic Potential of Labelled Somitic Cells in the Limbs --- p.29 / Chapter 2.3.4. --- Chimeaic Limb Culture --- p.30 / Chapter 2.4. --- Discussion --- p.32 / Chapter 2.4.1. --- Relationship Between the Somites and the Limb Musculature in Rat Embryos --- p.33 / Chapter 2.4.2. --- Myogenic Potential of Somitic Cells in the Mouse Limb Bud --- p.36 / Chapter 2.4.3. --- The Regulatory Potentials of Mammalian Somites --- p.37 / Chapter Chapter 3 --- The Migration of Somitic Cells into the Mammalian Fore- limb Bud / Chapter 3.1. --- Introduction --- p.52 / Chapter 3.2. --- Materials and Methods --- p.57 / Chapter 3.2.1. --- Embryo Collection --- p.57 / Chapter 3.2.2. --- Embryo Culture and Analysis of Cultured Embryos --- p.58 / Chapter 3.2.3. --- Experimental Series I --- p.58 / Chapter A. --- Micro-injection of DiI --- p.58 / Chapter B. --- Explant Cultures of Rat Fore-limb Bud --- p.59 / Chapter C. --- Histology and Immunohistochemistry --- p.60 / Chapter 3.2.4. --- Experimental Series II --- p.61 / Chapter A. --- Preparation of Conditioned and Unconditioned Medium --- p.61 / Chapter B. --- Coating of Nucleopore Membrane with Fibronectin --- p.62 / Chapter C. --- Preparation of Somitic Cells --- p.62 / Chapter D. --- Analysis of Chemotatic Effect --- p.63 / Chapter 3.2.5. --- Experiment Series III --- p.64 / Chapter A. --- Micro-injection of Latex Beads --- p.64 / Chapter B. --- Isolation of Somatopleure and Transplantation --- p.64 / Chapter C. --- "Somite Isolation, Labelling, and Transplantation" --- p.65 / Chapter D. --- Histology --- p.65 / Chapter 3.3. --- Results --- p.66 / Chapter 3.3.1. --- Development of Embryos In vitro --- p.66 / Chapter 3.3.2. --- Experimental Series I --- p.66 / Chapter A. --- Distribution of Somitic Cells in DiI Injected Embryos --- p.66 / Chapter B. --- Histogenetic Potential of Limb Explants Cultured Under the Kidney Capsule --- p.68 / Chapter C. --- Histogenetic Potential of Limb Explants Cultured In vitro --- p.69 / Chapter 3.3.3. --- Experimental Series II --- p.70 / Chapter A. --- Chemotatic Behaviour of Somitic Cells --- p.70 / Chapter 3.3.4. --- Experimental Series III --- p.71 / Chapter A. --- Ability of Latex Beads to Invade the Limb Bud --- p.71 / Chapter B. --- Distribution Pattern of Somatopleural and Somitic Cells --- p.71 / Chapter 3.4. --- Discussion --- p.75 / Chapter 3.4.1. --- Experimental Series I --- p.75 / Chapter A. --- Distribution of Somitic Cells in DiI-Injected Rat Embryos --- p.75 / Chapter B. --- Histogenetic Potential of Rat Fore-limb Bud --- p.77 / Chapter 3.4.2. --- Experimental Series II --- p.80 / Chapter A. --- Chemotatic Behaviour of Somitic Cells --- p.80 / Chapter 3.4.3. --- Experimental Series III --- p.80 / Chapter A. --- Ability of Latex Beads to Invade the Limb Bud --- p.80 / Chapter B. --- Ability of Somatopleure and Somite to Invade Limb Bud --- p.83 / Chapter 3.4.4. --- Conclusion --- p.83 / References --- p.106 / Appendix --- p.113

by Tang Mei Kuen. / "May 1999." / Thesis (Ph.D.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (p. 134-179). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

Wong, Wan Man. / Thesis submitted in: December 2006. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 85-98). / Abstracts in English and Chinese. / Abstract --- p.i / Abstract in Chinese --- p.iii / Acknowledgements --- p.v / Lists of Figures and Tables --- p.vi / Table of Abbreviations --- p.xi / Table of Contents --- p.xv / Chapter Chapter I --- Introduction / Chapter 1.1 --- Brain and Reproductive Organ Expressed Gene --- p.1 / Chapter 1.2 --- Programmed cell death --- p.4 / Chapter 1.3 --- Limb development in mouse --- p.8 / Chapter 1.4 --- Role of BRE in apoptosis --- p.12 / Chapter 1.5 --- Role of programmed cell death in interdigital tissue regression --- p.14 / Chapter 1.6 --- Aim of study --- p.17 / Chapter Chpater II --- Materials and methods / Chapter 2.1 --- Mice --- p.18 / Chapter 2.2 --- In-situ hybridization / Chapter 2.2.1 --- Histology --- p.18 / Chapter 2.2.2 --- Preparation of riboprobe for in-situ hybridization --- p.19 / Chapter 2.2.3 --- In-situ hybridization --- p.20 / Chapter 2.3 --- Interdigital tissue culture --- p.21 / Chapter 2.4 --- Gene interference / Chapter 2.4.1 --- Construction of Bre-siRNA --- p.22 / Chapter 2.4.2 --- siRNA transfection of cultured interdigital cells --- p.23 / Chapter 2.5 --- Semi-quantitative RT-PCR / Chapter 2.5.1 --- Sample collection of interdigital cells and explants --- p.23 / Chapter 2.5.2 --- RNA isolation and extraction --- p.24 / Chapter 2.5.3 --- Reverse-transcription and cDNA synthesis --- p.25 / Chapter 2.5.4 --- Polymerase chain reaction --- p.26 / Chapter 2.6 --- Assay of cell viability by MTT --- p.28 / Chapter 2.7 --- Comparative proteomics --- p.30 / Chapter 2.7.1 --- Collection of interdigital cells --- p.30 / Chapter 2.7.2 --- Preparation of cell lysate --- p.31 / Chapter 2.7.3 --- Assay of protein concentration in cell lysate --- p.31 / Chapter 2.7.4 --- Two-dimensional gel electrophoresis --- p.33 / Chapter 2.7.5 --- Protein identification by mass fingerprinting --- p.36 / Chapter 2.8 --- Statistical Method --- p.38 / Chapter Chapter III --- Results / Chapter 3.1 --- Spatial and temporal expression of Bre in murine embryonic hindlimbs --- p.39 / Chapter 3.2 --- Expression of Bre isoforms in interdigital tissues --- p.45 / Chapter 3.3 --- Silencing of Bre expression by siRNA in interdigital cells --- p.49 / Chapter 3.4 --- Effect on viability of Bre-silenced interdigital cells by siRNA --- p.51 / Chapter 3.5 --- Comparative proteomic profile of Bre-silenced interdigital cultured cells --- p.53 / Chapter 3.6 --- Identification of proteins that were differentially expressed by MALDI- TOF --- p.71 / Chapter 3.7 --- The mRNA levels of proteins identified that were differentially expressed --- p.74 / Chapter Chapter IV --- Discussion --- p.77 / References --- p.85 / Appendices --- p.99 / Publication --- p.108