Flowering plants require conspecific pollen to reproduce but they often also receive heterospecific pollen, suggesting that pollinators carry mixed pollen loads. However, little is known about drivers of abundance, diversity or composition of pollen carried by pollinators. Are insect-carried pollen loads shaped by pollinator traits, or do they reflect available floral resources? We quantified pollen on 251 individual bees and 95 flies in a florally diverse community. We scored taxonomic order, sex, body size, hairiness and ecological specialization of pollinators, and recorded composition of available flowers. We used phylogenetically controlled model selection to compare relative influences of pollinator traits and floral resources on abundance, diversity and composition of insect-carried pollen. We tested congruence between composition of pollen loads and available flowers. Pollinator size, specialization and type (female bee, male bee, or fly) described pollen abundance, diversity and composition better than floral diversity. Pollen loads varied widely among insects (10–80,000,000 grains, 1–16 species). Pollen loads of male bees were smaller, but vastly more diverse than those of female bees, and equivalent in size but modestly more diverse than those of flies. Pollen load size and diversity were positively correlated with body size but negatively correlated with insect ecological specialization. These traits also drove variation in taxonomic and phylogenetic composition of insect-carried pollen loads, but composition was only weakly congruent with available floral resources. Qualities of pollinators best predict abundance and diversity of carried pollen indicating that functional composition of pollinator communities may be important to structuring heterospecific pollen transfer among plants.

Animals in captivity can be deprived of performing some of their natural behaviours. Using enrichments may allow them to express a larger part of species-specific behaviour repertoire and with a better frequency distribution. This study focuses on three species of the family Herpestidae which live in captivity at Bioparc Valencia (Spain). The project aims to study the effect of environmental enrichment on the behaviour of meerkats, banded mongooses and dwarf mongooses in human care. To achieve this goal two different types of enrichmentswere tested: (1) a food enrichment with several variations and (2) an olfactory enrichment with the presentation of two new odours. The food enrichment aimed to increase foraging behaviour and the olfactory enrichment aimed to test if captive animals behave differently in the presence of a predator’s odour compared to a non-predator’s odour. Results revealed that foraging can increase up to 16% implementing enrichments and that success depends on the presence and quantity of food. On the other hand, animals did not seem to behave differently in the presence of both odours. The frequencies of behaviours and time spent interacting did not differ between these olfactory enrichments. I conclude that implementing enrichment programmes may ensure better welfare for captive animals.

Bees are the most important pollinators of flowering plants and are necessary for pollinating both wild plants and many of the crops that produce the food we eat. There are many different species of bees, with about 20,000 species worldwide and 4,000 species in the United States. Even though bees are important pollinators, there is still much we do not know about how many species there are and their biology. In order to better understand the species and their biology, I performed three projects that help fill these gaps by reviewing the species of a poorly known bee group, examining the different ways bees carry pollen, and then reviewing how bees gather pollen from flowers. To better understand bee diversity, I examined a group of species in the subgenus Heteroperdita in the genus Perdita (Andrenidae). I described nine species that were new to science, found the opposite sex of three species, and found that one species was a duplicate of a previously described species. This work increased the number of species in Heteroperdita to 22 and increased the number of species in the genus Perdita to 636. I then explored how pollen is carried back to the nest in two distantly-related bee groups, the genera Perdita and Hesperapis (Melittidae). I found that different species can carry pollen in one of three different ways: moist, dry, or glazed. Interestingly, how the bees carry pollen appears to depend on the shape and stickiness of the pollen grains that the bees prefer. I then reviewed how bees gather pollen from flowers. I combined previous research and my own observations of bees to classify the different pollen gathering behaviors into seven different types. I then examined why bees use different pollen gathering behaviors and provided a set terminology to refer to each behavior. Overall, this dissertation advances our knowledge of the diversity of bees and their relationships with flowers, which will support efforts to understand and conserve these important pollinators.

All plants contain primary and secondary compounds. Primary compounds are needed by plants and herbivores for maintenance, growth, and reproduction, while secondary compounds play roles as diverse as protecting plants from ultraviolet radiation, defenses against herbivores, pollination attraction, and stress resistance. Secondary compounds have nutritional and medicinal benefits for herbivores as well, especially when eaten in diverse combinations that complement one another. While complementarities among secondary compounds are an important but little understood area of plant-herbivore interactions, even less is known about how the sequences of eating plants with different compounds affects foraging behavior, though they may be critical. In three trials, I determined if the sequence in which lambs ate endophyte-infected tall fescue (alkaloids), birdsfoot trefoil (tannins), and alfalfa (saponins) affected their foraging behavior. When lambs grazed on monocultures they spent similar amounts of time grazing regardless of which forage they grazed. Lambs that grazed in a sequence of different forages tended to subsequently eat less alfalfa pellets in pens than lambs that grazed a monoculture, which suggests they better met their nutritional needs on mixtures than on monocultures. Likewise, lambs that grazed a monoculture of alfalfa or fescue spent more time grazing during the first 45 min than in the subsequent 45 min, while lambs that grazed alfalfa during the first 45 min and then fescue spent more time grazing in the subsequent 45 min, suggesting lambs satiate faster when they have fewer choices. While the foraging sequences I examined generally allowed animals to consume more than they would if they grazed in monocultures, there is still a need to further explore how different plants and foraging sequences influence the level of consumption by livestock of forages on pastures that contain various secondary compounds.

Traceability is defined as the ability to establish, record, and maintain dependency relations among various software artifacts in a software system, in both a forwards and backwards direction, throughout the multiple phases of the project’s life cycle. The availability of traceability information has been proven vital to several software engineering activities such as program comprehension, impact analysis, feature location, software reuse, and verification and validation (V&V). The research on automated software traceability has noticeably advanced in the past few years. Various methodologies and tools have been proposed in the literature to provide automatic support for establishing and maintaining traceability information in software systems. This movement is motivated by the increasing attention traceability has been receiving as a critical element of any rigorous software development process. However, despite these major advances, traceability implementation and use is still not pervasive in industry. In particular, traceability tools are still far from achieving performance levels that are adequate for practical applications. Such low levels of accuracy require software engineers working with traceability tools to spend a considerable amount of their time verifying the generated traceability information, a process that is often described as tedious, exhaustive, and error-prone. Motivated by these observations, and building upon a growing body of work in this area, in this dissertation we explore several research directions related to enhancing the performance of automated tracing tools and techniques. In particular, our work addresses several issues related to the various aspects of the IR-based automated tracing process, including trace link retrieval, performance enhancement, and the role of the human in the process. Our main objective is to achieve performance levels, in terms of accuracy, efficiency, and usability, that are adequate for practical applications, and ultimately to accomplish a successful technology transfer from research to industry.

Moist-soil wetlands that are seasonally flooded provide important habitats for waterfowl in the Mississippi Alluvial Valley (MAV). These wetlands often contain tall and dense vegetation that may constrain waterfowl use before natural openings form. During winters 2006–2009, I estimated abundances of waterbirds, seeds and tubers, and invertebrates in response to autumn, prelooding treatments of light disking, mowing, and no manipulation (control) of vegetation in 26 moist-soil wetlands in the MAV. Seeds and tubers were most abundant in control and mowed plots in late autumn. Decomposition was least and invertebrate abundance was greatest in control plots during winter. Dabbling ducks were most abundant in mowed and disked plots during winter. Lightly disked plots contained ~30% fewer seeds and tubers than mowed and control plots. In late winter, ~260 kg[dry]/ha of seeds and tubers remained among mowed, disked, and control plots. Therefore, autumn mowing of robust moist-soil vegetation can be used to create an interspersion of emergent vegetation and open water attractive to waterfowl and conserve waterfowl foods. Additionally, I identified 6 seed taxa that may not be used for food by dabbling ducks (i.e., Amaranthus spp., Cyperus odoratus, Eleocharis spp., Ipomoea spp., Jacquemontia tamnifolia, Sesbania herbacea) and estimated that removing these and other taxa not reported in diet literature in the MAV resulted in a ~31% reduction in estimated moist-soil food availability for ducks. In other experiments, I estimated that waterfowl reduced experimentally placed Japanese millet (Echinochloa frumentacea) to ~10 kg/ha and other natural seeds and tubers to ~170 kg/ha in experimental plots in mid-winter. However, waterfowl did not abandon wetlands or stop foraging when seed reduction ceased, suggesting residual abundances of seeds and tubers represented a food availability threshold (FAT). Using the median FAT value of 220 kg/ha from both experiments and removing 31% of seed mass that may not be consumed by dabbling ducks, results in a ~70% decrease in moist-soil seed availability in the MAV. Conservation planners should consider reducing the current estimates of seed and tuber availability and recommend increasing active management or implementation of additional managed, moist-soil wetlands in the MAV.

Grassland birds must have accessible, nutritional prey for nestlings which Conservation Reserve Program practices like CP33—Habitat Buffers for Upland Birds may provide. In 2008—2009, I monitored dickcissel nests in and around CP33 buffers at a farm in north-central Mississippi using video cameras to capture provisioning activities. I simultaneously observed foraging flights and measured distances traveled from nests. Orthopterans were the most commonly chosen prey, and dickcissels brought larger prey items when chicks were older. But, other changes in provisioning were not significantly related to nest age as I hypothesized. Also contrary to my initial hypotheses, provisioning at nests within buffers did not differ from non-buffer nests. CRP grasslands were equivalent to other available habitats. Provisioning rate and biomass decreased when an observer was present, and male feeding increased provisioning rate. Incorporating native warm-season grasses through conservation programs can increase nesting and foraging resources for dickcissels.

Foraging decisions of large herbivores can be affected by many factors and have great effect on both above- and below-ground ecosystems. Here, I determine to which degree temporal (seasonality), or spatial (habitat) factors influence the foraging behaviour of Svalbard reindeer (Rangifer tarandus platyrhynchus) and asses if small-scale foraging behaviour correlates with an animals’ patch residence time. I analysed behavioural observations on 15 Svalbard reindeer between summer and autumn 2022, recording both behaviour and habitat use. Using mixed-effects multinomial logistic regression, the interaction between seasonal change from summer to autumn and habitat best explained the variation in Svalbard reindeer foraging behaviour. The foraging behaviour “standing-grazing” was found to have the highest observed amount in all habitat types and increased over the growing season. Contrastingly, the foraging behaviour of “walking-grazing” had its highest abundance in the beginning of the study period, and then decreased over the course of the season for all analysed habitats. In addition, “walking-grazing” was found to be significantly correlated with patch residency time and decreased the longer an individual stayed in a patch. With presumable high plant quality early in the season, my results imply how Svalbard reindeer are greatly stationary foragers, but prioritizing to become relatively mobile when it is beneficial. Thus, highlighting how Svalbard reindeer respond dynamically to shifts in foraging conditions. These findings provide knowledge about what factors may impact the foraging behaviours of Arctic herbivores and helps contribute to a broader understanding of how changes in foraging behaviours may alter arctic ecosystems.

No description available.

No description available.