Spelling suggestions: "subject:"fakultät für chemie und pharmazie"" "subject:"fakultät für chemie und charmazin""
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Structural characterization of chaperone assisted folding and assembly of RuBisCOBharathi, Vasudeva Rao 12 March 2009 (has links) (PDF)
No description available.
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Mitochondriale Zielsteuerung durch N-terminale Signalpeptide sekretorischer ProteinePfeiffer, Natalie Verena Maria 13 December 2012 (has links) (PDF)
No description available.
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Regulation of integrin-linked kinase (ILK) stabilityRadovanac, Korana 05 July 2012 (has links) (PDF)
No description available.
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Mechanisms of rabies virus to escape the IFN systemRieder, Martina 07 March 2012 (has links) (PDF)
No description available.
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Graphenwachstum auf Ru(0001) in-situ STM-Untersuchungen zur TemperaturabhängigkeitDänhardt, Sebastian 26 June 2012 (has links) (PDF)
No description available.
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Charakterisierung des kardialen Beta-adrenergen PhosphoproteomsGöbel, Petra 24 February 2012 (has links) (PDF)
No description available.
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Targeted delivery of the theranostic sodium iodide symporter (NIS) for cancer gene therapyGrünwald, Geoffrey 26 June 2013 (has links) (PDF)
No description available.
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Novel dry powder inhalation system based on dispersion of lyophilisatesClaus, Sarah 18 December 2012 (has links) (PDF)
No description available.
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Multichromophore Farbsysteme auf Basis der Perylenbisimide mit linear und orthogonal orientierten ÜbergangsdipolmomentenWalter, Andreas Martin 13 July 2011 (has links) (PDF)
No description available.
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Protein particle analysisZölls, Sarah Elisabeth 26 July 2013 (has links) (PDF)
The aim of this thesis was to identify and evaluate critical factors for protein particle analysis and to apply this knowledge for the development of novel standardized protein-like particles. Thorough analysis of particles in therapeutic protein formulations is crucial due to regulatory requirements, the potential immunogenicity of protein aggregates and particles, and the need for quality and stability control of the product.
The introduction gives a comprehensive overview of analytical methods for particle characterization in therapeutic protein formulations based on the currently available literature. Within the thesis, the performance of novel techniques or instruments for (protein) particle counting, sizing, or characterization was assessed. Micro-Flow Imaging (MFI) and resonant mass measurement (RMM) were tested for the differentiation of protein particles and silicone oil droplets which is highly relevant especially for pharmaceutical products in prefilled syringes. Four different flow imaging microscopy systems (MFI4100, MFI5200, FlowCAM VS1, and FlowCAM PV) were subjected to a detailed investigation of particle quantification, characterization, image quality, differentiation of protein particles and silicone oil droplets, and handling of the systems.
A material screening of proteinaceous and non-proteinaceous materials for the development of novel standardized protein-like particles revealed gelatin and PTFE particles as promising materials for light-based applications. The density of protein particles, as a crucial particle parameter for weight-based techniques like RMM, was determined by two newly developed methods. The relevance of the refractive index (RI), which is closely related to transparency, was investigated and a novel method for RI determination of protein particles was developed. As protein particles became “invisible”, i.e. not detectable anymore by light-based systems at increased RI values - e.g. due to high protein concentration and/or sugars as excipients - potential solution strategies were evolved.
Taken together, this thesis provides new insight into the analysis of particles in therapeutic protein formulations. In this regard, potential candidates for the development of novel standardized protein-like particles identified in this study are very valuable and can help to improve protein particle analysis in the future.
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