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Several studies have reported that rating of perceived exertion (RPE) covaries more with blood lactate concentration (BLC) than other measures of relative exercise intensity. If BLC is used as the intensity criterion, then RPE could be used as a valid tool for exercise prescription. However, few have shown the relationship between RPE and BLC across a variety of graded exercise tests (GXTs) and exercise training settings. The purpose of this study was to determine if differences exist in RPEs at matched BLC between two GXTs and steady state exercise in the field setting. Thirteen healthy males (25 ± 5.3 yrs) completed two maximal treadmill protocols (Bruce and Balke) and one steady state exercise trial on a treadmill at two intensities which lasted approximately 7 minutes each. The intensities (40% & 70% max heart rate reserve) were below and above their ventilatory threshold. RPEs were recorded during the last minute of each workload of the field trial (FT). Immediately following the end of each workload, blood samples were collected for BLC analysis. RPEs from the FT were compared with RPEs from the GXTs at matched BI-Cs using a repeated measures ANOVA across exercise trials. The results show that there were no significant differences in RPEs at a matched BLC of 1.5 mM between the FTand and the Bruce and Balke GXT at the lower intensity, with the RPEs being 9.6 ± 1.7, 9.8 ± 2.6, and 10.2 ± 2.4, respectively. However, at the higher intensity, RPEs at a matched BLC of 3.0 mM were significantly different, with the FT < Bruce < Balke (11.9 ± 1.9, 13.5 ± 2.2 and 15.2 ± 2.0, p < 0.05). These results suggest that the relationship between RPE and BLC established during a GXT may not be transferable to the exercise training setting at exercise intensities in the typical prescription range of 50-85%. Therefore, using RPE for exercise prescription may not be an effective method for regulating exercise intensity according to BLC. / School of Physical Education

Neuromuscular electrical stimulation (NMES) has been shown to be effective for recovery of motor function following injury or pathology, however, NMES can impart rapid fatigue and the specific parameters of stimulation that maximize force output and delay the onset of fatigue remain unclear. Frequency, intensity, and pulse pattern are a few of the parameters that can be manipulated to achieve desired outcomes. Strong evidence supports the use of higher frequencies of stimulation to maximize performance of fatigued or paralyzed muscle. Likewise, several studies advocate the use of varied pulse patterns, rather than constant pulses, to maximize force output as well. Much of the research literature regarding NMES use with a pathological population involves spinal injured individuals. Much less is known about the effect of NMES on motor recovery, especially when delivered through varied pulse patterns, in the post-stroke population. The three studies contained herein addressed these issues. In Study 1, submaximal and supramaximal intensities of two variable frequency stimulation patterns and one constant frequency pattern were applied to the thenar muscles of a young healthy population. Results showed marked differences in force output between the two intensities. Submaximal stimulation enhanced the effect of the variable pulses and a greater force response was produced. In Study 2, the same three patterns were applied to the thenar muscles of a post-stroke group and an age-matched able-bodied group at submaximal intensities. Again, differences in force output were evident between the healthy and pathological group, and the variable pulses may have further depressed already weakened hemiplegic muscle. Study 3 compared the effects of a NMES rehabilitation program using a high (40 Hz) and a low (20 Hz) frequency to determine if task-specific improvements were related to frequency used. Results showed that those in the high frequency condition demonstrated greater improvements in strength, dexterity and force accuracy; those in the low frequency condition showed improvements in motor endurance. The results of this work suggest that the intensity, frequency and stimulation pattern of NMES used have a significant impact on the resultant muscle contraction and functional skills gained following stroke and should be carefully considered when implementing a clinical regimen for motor recovery.

This study examined the effects of 14 days of L-carnitine supplementation on blood and muscle lactate concentrations, and carnitine fractions, during high intensity sprint cycling exercise. Eight subjects performed three experimental trials - control I (CON I, 0 days), control II (CON II, 14 days), and L-carnitine (LCN, 28 days). Each trial consisted of a 4 min ride at 90% VO2max, followed by a rest period of 20 min, and then 5 x 1 min rides at 115% VO2max (2 min restbetween each). Following CON II, all subjects began dietary supplementation of L-carnitine for a period of 14 days (4 g/day). L-carnitine supplementation had no significant effect on either muscle carnitine or lactate concentrations following the 4 min 90% ride. Plasma total acid soluable and free carnitine concentrations were significantly higher at all time points following supplementation. Differences observed in blood hydrogen ion and lactate concentrations between CON I and CON II appear to be the result of an order effect. The data from the present investigation indicate that L-carnitine supplementation has no significant effect on blood or muscle lactate accumulation following high intensity sprint cycling exercise. / School of Physical Education

Fatigue induces changes to running form; therefore movement is not as effective or efficient. Reducing the amount of fatigue or its effects on form would be ideal to improve performance while running. Compressive clothing has unknown effects on musculature, however it claims to reduce fatigue. The aim of this study was to see the changes in running form while running to exhaustion and to see how compression tights can effect these changes. Eleven runners ran at their current five-kilometer race pace on a treadmill to voluntary exhaustion in a repeated measures design wearing compression tights and regular shorts while their kinematics, kinetics, heart rate and rate of perceived exhaustion were recorded. There was not a significant difference in time to exhaustion. Fatigue general effects were significant from beginning to end in knee and ankle angle at initial contact with the knee becoming less extended and the ankle less dorsiflexed. Vertical ground reaction loading rate and impact peak were significantly different from beginning to mid point and beginning to end across conditions. Heart rate and rate of perceived exertion increased significantly with fatigue as well in both conditions. Condition effects were significant in stride length and rate with a decreased stride length with compression tights and an increased stride rate with compression tights. The hip experienced a decreased range of motion in the compression tights compared to running shorts. These results indicate that there are effects of fatigue on performance and differences between conditions. These differences did not affect the overall outcome of run as measured in time to exhaustion. / School of Physical Education, Sport, and Exercise Science

Despite extensive research into the concept of mental fatigue there is as yet no “gold standard” definition or measurement technique available. Because of this a large amount of fatigue-related errors are still seen in the workplace. The complexity of the problem lies with the inability to directly measure mental processes as well as the various endogenous and exogenous factors that interact to produce the experienced fatigue. Fatigue has been divided into sleep-related and task-related fatigue; however the task-related aspect is evident both during normal waking hours as well as during periods of sleep deprivation, therefore this aspect is considered important in the understanding of fatigue in general. The concept of task-related fatigue has further been divided into active and passive fatigue states; however differentiation between the two requires careful consideration. Various physiological measures have been employed in an attempt to gain a better understanding of the mechanisms involved in the generation of fatigue, however often studies have produced dissociating results. The current study considered the task-related fatigue elicited by a tracking task requiring sustained attention, in order to evaluate the usefulness of various cardiovascular and oculomotor measures as indicators of fatigue. A secondary aim was to determine whether the behavioural and physiological parameter responses could be used to infer the type of fatigue incurred (i.e. an active versus passive fatigue state) as well as the energetical mechanisms involved during task performance. A simple driving simulator task was used as the main tracking task, requiring constant attention and concentration. This task was performed for approximately two hours. Three experimental groups (consisting of 14 subjects each) were used: a control group that performed the tracking task only, a group that performed a five minute auditory memory span task concurrently with the driving task after every 20 minutes of pure driving, and a group that performed a visual choice reaction task for five minutes following every 20 minute driving period. The secondary tasks were employed in order to evaluate the extent of resource allocation as well as arousal level. Performance measures included various driving performance parameters, as well as secondary task performance. Physiological measures included heart rate frequency (HR) and various time- and frequency-domain heart rate variability (HRV)parameters, pupil dilation, blink frequency and duration, fixations, and saccadic parameters as well as critical flicker fusion frequency (CFFF). The Borg CR-10 scale was used to evaluate subjective fatigue during the task, and the NASA-TLX was completed following the task. A decline in driving performance over time was supplemented by measures such as HR, HRV and pupil dilation indicating an increase in parasympathetic activity (or a reduction in arousal). An increase in blink frequency was considered as a sign of withdrawal of attentional resources over time. Longer and faster saccades were also evident over time, coupled with shorter fixations. With regards to the secondary task influence, the choice RT task did not affect any behavioural or physiological parameters, thereby contesting the active fatigue theory of resource depletion, as well as implying that the increase in demand for the same resources used by the primary task was insufficient to affect the state of the subjects. The increased load elicited by the memory span task improved driving performance and increased measures of HR, HRV, pupil dilation and blink frequency. Some of these measures produced opposite effects to what was expected; an attempt to explain the dissociation of the various physiological parameters was expressed in terms of arousal, effort and resource theories. Overall, the results indicate that the fatigue and/or reduced arousal accompanying a monotonous sustained attention task can, to some degree, be alleviated through intermittent performance of a secondary task engaging mental resources other than the ones used for the primary task. The degree to which such a task is beneficial, however, requires careful consideration as while an immediate increase in arousal and primary task performance is noted, the impact of the task on general attentional resources may be detrimental in the case of reacting should an emergency situation occur.

Achieving adequate and appropriate recovery from exercise is essential in ensuring optimal performance during repeated bouts of exercise. The use of various recovery interventions has become popular in an attempt to enhance subsequent performance and accelerate post-exercise recovery. The application of various post-exercise hydrotherapy interventions has become increasingly popular, however, the majority of current recovery practices appear to be based largely on anecdotal evidence as opposed to rigorous scientific research or evidence based findings. Physiologically, various hydrotherapy protocols have been shown to affect the body via fluid shifts (interstitial to intravascular space), changes in blood flow and cardiovascular function, and reductions in oedema. The possible psychological effects of water immersion must also be considered, with athletes commonly reporting reduced sensations of fatigue and soreness following immersion. Current literature suggests both hydrostatic pressure and water temperature to be important factors influencing the success of hydrotherapy. The overall aim of the present thesis was to enhance current knowledge and understanding with regards to the physiological and performance effects of various forms of hydrotherapy, used as a post-exercise recovery intervention. Initially, four cold water immersion interventions were compared to active recovery, performed between two bouts of high intensity cycling in hot environmental conditions. Effectiveness of recovery was determined via performance in a subsequent exercise bout; in addition, core body temperature, lactate, and heart rate were recorded. The remaining studies were designed to investigate the effects of cold water immersion, hot water immersion, contrast water therapy, and passive recovery 4 (control) following exercise-induced fatigue and exercise-induced muscle damage. Rate of recovery was assessed through changes in performance, core body temperature, thigh girths, blood markers, and perceived exertion/soreness. The results of the combined studies indicate cold water immersion to be more effective than active recovery when performed immediately post-exercise between two bouts of high intensity cycling in hot environmental conditions. Additionally, both cold water immersion and contrast water therapy were effective in aiding recovery from exercise-induced fatigue and exercise-induced muscle damage. Performance variables indicated an improved maintenance or return of performance following these recovery protocols. The present studies have provided additional information to the limited knowledge base regarding the effect of post-exercise hydrotherapy interventions, specifically, the effect of such interventions on subsequent athletic performance. In conclusion, cold water immersion and contrast water therapy appear to be superior to hot water immersion, active recovery, and passive recovery following fatiguing and muscle damaging exercise. Functional and physiological recovery was enhanced following the use of these two recovery protocols.

The purpose of this study was to examine VT and LT in boys and men. Eight boys (10-11 years) and nine men (18-30 years) completed a graded exercise test on a cycle ergometer. A two-way (group x threshold) ANOVA compared physiological responses (V02 1/min and ml/kg/min, percentage of V02max, and HR) at VT and LT. Statistical significance was set at P < 0.05. No significant interaction was observed. Significant main effects for group included a higher V02 (1/min) in the men, and a higher percentage of VO2max in the boys. Significant main effects for threshold showed all variables were greater at VT than LT. Within each group, all variables were significantly higher at VT than LT. For the boys and all subjects together, significant correlations between thresholds were observed for V02 (1/min and ml/kg/min) and HR, but not percentage of VO2max. For the men alone, no significant correlations were found. Together, these results suggest that physiological changes associated with LT may contribute to the onset of VT, and the occurrence of the thresholds (expressed as a percentage of VO2max) declines with maturation. / School of Physical Education

Road traffic accidents are a serious burden to the health systems of many countries especially in South Africa. Research aimed at reducing traffic related accidents is of importance as traffic crashes are rated as the second leading cause of fatalities in South Africa and ninth in the world. Despite the extensive efforts into research and development of new technology, driver fatigue still remains a cause of vehicle accidents worldwide. Fatigue plays a role in up to 20% of vehicle accidents with many being serious or fatal. Numerous coping behaviours are employed by drivers to counteract the negative effects of fatigue. The most common coping behaviours include taking short naps, talking to passengers, listening to the radio, opening windows and drinking stimulants. Driving breaks have long been identified as an effective countermeasure against fatigue. Most research done in driving breaks has investigated the duration of the breaks, activity undertaken during the break and the frequency of the breaks taken outside the vehicle. However limited literature is available on the effectiveness of breaks in counteracting the effects of fatigue. The objective of the current study was aimed at assessing whether short duration breaks are an effective countermeasure against fatigue. Physiological, neurophysiological, subjective and performance measures were used as indicators for fatigue. Additional focus of the research was determining whether breaks were more or less effective at counteracting the effects of fatigue at different times of day. Twelve participants were recruited for the study, six males and six females. The participants were required to perform a driving task on a simulator for 90 minutes. The study consisted of four independent conditions, namely driving during the day with breaks, driving during the day without breaks, driving during the night with breaks and driving during the night without breaks. The without breaks conditions were similar except that they occurred at different times of the day, one session at night and the other session during day time, as was the case for the conditions with breaks. The driving task used in the current study was a low fidelity simulator tracking task. The participants were required to follow a centre line displayed on a tracking path as accurately as possible. The measurements that were recorded in this study included physiological, performance, subjective and neurophysiological. Physiological measures included heart rate and heart rate variability (frequency domain) and core body temperature. The ascending threshold of the critical flicker fusion frequency was the only neurophysiological measurement included in the current investigation. Performance was quantified by mean deviation from a centre line participants were meant to track. Two rating scales were used: Karolinska sleepiness scale and the Wits sleepiness scale were used for the measurement of subjective sleepiness. Heart rate, heart rate variability and mean deviation were measured continuously throughout the 90 minute driving task. Critical flicker fusion frequency, temperature and the subjective scales were measured before and after the 90 minute driving task. The results indicated that the short duration breaks during day time had a positive effect on driving performance; however the breaks at night had a negative effect on driving performance. Heart rate was higher during the day compared to night time and the heart rate variability high frequency spectrum values were lower during the day condition, to show the activation of the sympathetic nervous system which is characteristic of day time. The night conditions had lower heart rate values and higher heart rate variability high frequency values, which show the activation of the parasympathetic nervous system which is dominant during periods of fatigue and night time. Subjective sleepiness levels were also higher at night compared to day time.