Global ETD Search

1	Evaluation of the Efficacy of DNA Sequencing and Microhistological Analysis for Determining Diet Composition in Ungulates January 2012 (has links) abstract: An understanding of diet habits is crucial in implementing proper management strategies for wildlife. Diet analysis, however, remains a challenge for ruminant species. Microhistological analysis, the method most often employed in herbivore diet studies, is tedious and time consuming. In addition, it requires considerable training and an extensive reference plant collection. The development of DNA barcoding (species identification using a standardized DNA sequence) and the availability of recent DNA sequencing techniques offer new possibilities in diet analysis for ungulates. Using fecal material collected from controlled feeding trials on pygmy goats, (Capra hicus), novel DNA barcoding technology using the P6-loop of the chloroplast trnL (UAA) intron was compared with the traditional microhistological technique. At its current stage of technological development, this study demonstrated that DNA barcoding did not enhance the ability to detect plant species in herbivore diets. A higher mean species composition was reported with microhistological analysis (79%) as compared to DNA barcoding (50%). Microhistological analysis consistently reported a higher species presence by forage class. For affect positive species identification, microhistology estimated an average of 89% correct detection in control diets, while DNA barcoding estimated 50% correct detection of species. It was hypothesized that a number of factors, including variation in chloroplast content in feed species and the effect of rumen bacteria on degradation of DNA, influenced the ability to detect plant species in herbivore diets and concluded that while DNA barcoding opens up new possibilities in the study of plant-herbivore interactions, further studies are needed to standardize techniques and for DNA bar-coding in this context. / Dissertation/Thesis / M.S. Applied Biological Sciences 2012 Wildlife management Nutrition Animal sciences Diet analysis DNA Barcoding Fecal analysis Herbivore diet Microhistology Ungulate
2	Effects of white-tailed deer herbivory on a tallgrass prairie remnant Gooch, Scott 11 January 2010 (has links) A study was conducted to determine what impact high white-tailed deer (Odocoileus virginianus) densities were having on the native grasslands of a tallgrass: aspen forest tract embedded within an agro-urban setting. Due to excessive spring moisture, row-crops were unavailable the first year. Using microhistological fecal analysis to determine dietary composition, deer were assessed to be placing the site’s favoured native plant species at risk of extirpation. Measuring woody stem abundance and height along and near the prairie: forest ecotone, deer were found to restructure woody growth but not directly influence encroachment rates. Indirectly, however, deer facilitated forest encroachment and prairie degradation through seed dispersal, nitrogen deposition, gap-dynamics, and trampling. Comparing dietary composition to nutritional data, deer grazed to maximize fitness, selecting foods high in IVDMD, minimizing energy expenditure, and optimizing CP. High crop CP was offset by intensively grazing particular native plants. ADF was an effective nutritional marker, not AIA. white-tailed deer herbivory fecal analysis nutrient cycling forest encroachment foraging strategy disturbance index ADL nutritional markers row-crops grazing preferences gap dynamics seed propagation ecological fitness native plants forbs restoration ADF CP AIA NDF deer management ecological management IVDMD overabundance tallgrass energetics forbs
3	Effects of white-tailed deer herbivory on a tallgrass prairie remnant Gooch, Scott 11 January 2010 (has links) A study was conducted to determine what impact high white-tailed deer (Odocoileus virginianus) densities were having on the native grasslands of a tallgrass: aspen forest tract embedded within an agro-urban setting. Due to excessive spring moisture, row-crops were unavailable the first year. Using microhistological fecal analysis to determine dietary composition, deer were assessed to be placing the site’s favoured native plant species at risk of extirpation. Measuring woody stem abundance and height along and near the prairie: forest ecotone, deer were found to restructure woody growth but not directly influence encroachment rates. Indirectly, however, deer facilitated forest encroachment and prairie degradation through seed dispersal, nitrogen deposition, gap-dynamics, and trampling. Comparing dietary composition to nutritional data, deer grazed to maximize fitness, selecting foods high in IVDMD, minimizing energy expenditure, and optimizing CP. High crop CP was offset by intensively grazing particular native plants. ADF was an effective nutritional marker, not AIA. white-tailed deer herbivory fecal analysis nutrient cycling forest encroachment foraging strategy disturbance index ADL nutritional markers row-crops grazing preferences gap dynamics seed propagation ecological fitness native plants forbs restoration ADF CP AIA NDF deer management ecological management IVDMD overabundance tallgrass energetics forbs

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