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Effect of Surface Wettability, Morphology and Chemistry on the Biocompatibility of Laser Textured Titanium SurfacesZhao, Xun 04 June 2021 (has links)
Titanium has been used in bio-medical implants for decades due to its superior biocompatibility. To improve the osseointegration of dental and orthopaedic implants, various surface modification techniques have been used including laser surface texturing. In particular, short-pulsed lasers, such as femtosecond and picosecond lasers, are widely used for surface modification.
In this thesis, commercially pure Ti surfaces are modified by a femtosecond laser to explore the relationship between surface topography, surface chemistry, surface wettability, and biocompatibility with the goal of improving the osseointegration of implants. The laser textured surfaces consist of 1μm wide grooves spaced 10 μm, 4.8 μm, 2.4 μm and 1.2 μm apart. Gradient configurations where the groove spacing varies are also investigated. Surface morphology was characterized using Optical Microscopy (OM), Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM).
A custom-build contact angle measurement apparatus is used to investigate the wettability of the laser textured surfaces using the sessile drop method. Freshly laser-treated commercially pure Ti surfaces are found to be super-hydrophilic and become hydrophobic over time when exposed to air. The presence of grooves can accelerate the evolution of the contact angle over time, and introduces anisotropy in the wetting behavior (along vs. across the grooves). The hydrophilicity of laser treated surfaces can be retained by storing samples in ethanol.
X-ray Photoelectron Spectroscopy (XPS) shows that the relative carbon content increases over time when Ti samples are exposed to air, which results in the subsequent evolution of the contact angle and cell response to laser textured Ti surfaces. Besides, laser treatment promotes the oxidation of pure Ti, and the product, TiO2, is responsible for the better biocompatibility.
In vitro experiments using MG 63s osteoblast-like cells are implemented on laser-treated Ti surfaces and polished surfaces (control) with 1 day, 3 days and 7 days of cell culture. The best cell outcome was obtained by storing samples in air for 1 week, where storing for shorter or longer times resulted in the worst outcome, especially in the early stages of cell adhesion. There does not appear to be a direct link between wettability and the fate of cells on Ti surfaces.
Indeed, while samples stored in air and ethanol have drastically different contact angle measurements (the former being hydrophobic and the latter hydrophilic), the cell behavior was unaffected.
In addition, while wettability and laser treatment can affect the early stages of cell adhesion, they do not have a strong effect on the number of cells at longer incubation times (3 and 7 days). Laser machining does however affect the cell morphology and alignment, where cells preferentially align themselves parallel to the direction of the laser machined grooves with an elongated morphology.
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