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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.

Regulation of cellular senescence in human fibroblasts /

Benanti, Jennifer Ann. January 2003 (has links)
Thesis (Ph. D.)--University of Washington, 2003. / Vita. Includes bibliographical references (leaves 106-118).

Role of interleukin-15 and nitric oxide expression in chronic inflammatory disease

Leung, Bernard P. January 1998 (has links)
No description available.

Regulation of prostaglandin E₂ synthesis in human gingival fibroblasts /

Yucel-Lindberg, Tülay, January 1900 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 6 uppsatser.


ULREICH, JUDITH BABB. January 1987 (has links)
Fibronectin (FN) is a critical component of the extracellular matrix (ECM) of fibroblasts and muscle cells and contributes to the maintenance of cell cytoskeleton, shape, migration, growth and differentiation. Extensive accumulation of both collagen and FN occurs in skeletal muscle of patients with Duchenne's muscular dystrophy (DMD). Several researchers have recently claimed that FN can interfere with proper muscle differentiation. Our hypothesis is that DMD fibroblasts or muscle cells fail to regulate properly the type, synthesis, or secretion of FN and that the increased accumulation FN in the ECM of fibroblasts in muscle may interfere with myogenesis, thus presenting the typical picture of regenerating but largely non-functional muscle in DMD. This was tested by labeling cultures of fibroblasts from DMD patients and controls with ³⁵S-methionine and quantitating basal levels of FN synthesis and degradation by immunoprecipitation. Collagen, FN and other components were measured in DMD sera and the metabolic effects of culturing DMD and control fibroblasts in the presence of DMD sera were studied. Muscle biopsies from DMD patients were examined for factors which might contribute to the accumulation of connective tissue. Increased levels of FN were measured in sera and cultured fibroblasts from DMD patients. Concomitant increases in other ECM components (collagen, glycosaminoglycans) were measured. The FN accumulation is at the expense of other cellular proteins as RNA and protein synthesis are reduced in DMD fibroblasts. Protein degradation studies indicate that reduced catabolism of FN may account for significant elevations in cells and sera. DMD sera cultured on control fibroblasts caused metabolic alterations reminiscent of dystrophic cells (increased FN and collagen accumulation, decreased RNA synthesis). Many of the observed metabolic changes are age-related in DMD, increasing in older patients. These results suggest that overproduction by fibroblasts and increased serum levels of components of the ECM in DMD may influence the accumulation of connective tissue in DMD muscle. DMD may not be of myopathic origin but an increased compartment of fibroblasts and macrophages as observed in DMD muscle may result from chemotactic mobilization caused by elevated FN and/or collagen in the tissue.

Collagen metabolism by fibroblasts from normals and individuals with Osteogenesis Imperfecta

Fraser, Judith. January 1980 (has links)
Collagen production by skin fibroblast cell strains from normal subjects and age-matched patients with the mendelian disorder--Osteogenesis Imperfecta (OI)--was studied in culture. / Number of generations in culture, phase of growth, labelling times, and site of biopsy did not influence collagen production by normal skin fibroblasts. / OI cell strains from patients with dominantly inherited OI have abnormal morphology and growth in culture. The ratio of Type I to protype III collagen is reduced in OI Types I, II and IV (Sillence classification). Type III OI could not be distinguished from normal strains by the analysis used. From the collagen phenotype (biochemical parameters) we were able to distinguish different OI phenotypes and to correlate these with clinical phenotypes. One form of OI Type I produces an unstable collagen that is degraded to small peptides in culture.

Studies on the enzyme defects in G M : gangliosidosis Types 1 and 2.

Powell, Elizabeth January 1971 (has links)
No description available.

Characterization of mutations in pediatric mitochondrial myopathies

Slipetz, Deborah M. January 1990 (has links)
Mitochondrial myopathies are a group of diverse neuromuscular disorders. Defects in electron transport chain (ETC) subunits have been implicated in pediatric and adult onset cases. Skin fibroblasts from four patients were studied to elucidate the biochemical defects. / Cells from two patients with ETC complex I deficiency, showed reduced oxidation of alanine with normal oxidation of succinate. Analysis of complex I subunits indicated deficient synthesis of the 20 kDa subunit in the severely affected patient. In the milder patient, subunit abnormalities were not detected. / Fibroblasts from a patient with facioscapulohumeral disease (FSHD), showed reduced oxidation of alanine and succinate through the ETC. / A fourth patient, with decreased activity in several complexes in muscle and liver, was found to have a heteroplasmic mtDNA population in fibroblasts. / These studies exemplify the heterogeneity of mitochondrial myopathies and demonstrate the utility of fibroblasts in the investigation of these disorders.

Characterization of normal and androgen resistant-genital skin fibroblasts using high-resolution two-dimensional gel electrophoresis

Schwartz, Anne. January 1985 (has links)
No description available.

The effect of intermittent tensile strain on RANKL, OPG, M-CSF and IL-1β expression by periodontal ligament fibroblasts in vitro

Gaffey, Benjamin James, n/a January 2007 (has links)
Mechanical stress has been shown to play a role in bone remodelling during orthodontic tooth movement. Receptor activator of nuclear factor kβ - ligand (RANKL), osteoprotegerin (OPG), monocyte colony stimulating factor (M-CSF) and interleukin 1-β (IL-1β) play key roles in the regulation of bone remodelling, but the role of these cytokines in orthodontic tooth movement is poorly understood. Aim: The aim of this experiment was to examine the response of periodontal ligament (PDL) fibroblasts in monolayer culture to intermittent tensile stress as regards RANKL, OPG, M-CSF and IL-1β production. Methods: Human PDL fibroblasts were dissected from premolars extracted for orthodontic purposes. Explants were seeded out in 1cm wells and grown to confluence in Dulbecco�s modification of Eagle�s medium, containing 10% foetal calf serum and antibiotics, at 37�C in a humidified atmosphere of 5% CO₂/95% air. Upon reaching confluence, the cells were passaged into sequentially larger flasks. Fibroblasts were passaged 6 times. After reaching confluence in T175 flasks, the cells were detached and plated at a cell density of 10⁵/dish in 35mm Bioflex� Plates coated with type 1 collagen. The cells were placed under a continuous uni-axial strain of 12% for 6s of every 90s by a Flexercell FX 4000C[TM] for 0, 12, 24 and 48 hours. Cells were then detached and stored in RNAlater. Quantitative RT-PCR was used to determine the mRNA of the cytokines of interest. Results: Tensile force led to the down regulation of mRNA expression for OPG and IL-1β at 12 and 24 hours respectively, while M-CSF was up-regulated at 6 hours. RANKL was not detected at a significant level for quantification. Conclusion: This osteoclastic-type response indicates the complexity of mechanotransduction in an in vitro setting. Acknowledgments: This research was supported by the New Zealand Dental Research Foundation, the New Zealand Lottery Grants Board and the New Zealand Association of Orthodontists.

Observations on testosterone metabolism in cultured human fibroblasts.

Finkelberg, Rosanna January 1970 (has links)
No description available.

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