Thesis (Ph. D.)--University of New South Wales, 2004. / Also available online.

Thesis (M.S.)--Virginia Polytechnic Institute and State University, 1994. / Vita. Abstract. Includes bibliographical references (leaves 70-96). Also available via the Internet.

Diss. Naturwiss. ETH Zürich, Nr. 6945, 0000. Ref.: Leisinger, T. ; Korref.: Guggenheim, B.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1967. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin, Madison, 1974. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1969. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliography.

Thesis (Ph. D.)--University of Texas at Austin, 2005. / Vita. Includes bibliographical references.

Thesis (Ph. D.)--Ohio State University, 2007. / Title from first page of PDF file. Includes bibliographical references (p. 128-138).

Glucocorticoids are known to affect metabolic activities of cells. The mechanism of glucocorticoid actions in adult human dermal and mouse L-929 fibroblasts have yet to be fully ascertained. This study endeavors to examine the effects of one glucocorticoid, triamcinolone acetonide, on cellular proliferation and collagen synthesis and to compare such effects in the human and mouse cell lines. Cellular proliferation and collagen synthesis are analyzed and quantitated by cell counts and selective digestion of the protein by bacterial collagenase, respectively. Further analysis of collagen synthesis is provided by polyacrylamide gel electrophoresis. One-tenth triamcinolone acetonide per ml suppresses cellular proliferation of mouse L-929 fibroblasts. Proline incorporation into total and collagenase-sensitive protein is enhanced in the cell layer; that of medium is altered inconsistently. Polyacrylamide gel electrophoresis of proteins treated with pepsin show the abolition of total and collagenase-sensitive protein in the cell layer. Aberrations in hydroxylation and/or deformation in physical structure of protein may confer greater susceptibility to pepsin digestion. Cellular proliferation and proline incorporation into total and collagenase-sensitive protein of adult human dermal fibroblasts are affected inconsistently by the same dose of triamcinolone acetonide. Except for the consistent suppression of cellular proliferation in the murine L-929 fibroblasts by triamcinolone acetonide, all observations pertaining to human dermal fibroblasts are incompatible with those obtained by other workers. Manipulation of culture conditions and glucocorticoid treatment dictate, to a large extent, the kind of responses observed. This could account for the wide variability and frequent contradictory findings reported in the literature. / Pharmaceutical Sciences, Faculty of / Graduate

The purpose of this investigation was to provide pure cultures of normal human prostatic epithelium free of fibroblasts in order to study malignant conversion by chemical carcinogens. Normal epithelial cells were needed because this was the cell type implicated in prostatic malignancies of human subjects. Unfortunately fibroblasts grew faster than epithelial cells so that cultures were always overgrown with connective tissue elements. It was considered important to find a method which would eliminate fibroblasts so that normal epithelial cells could grow out in pure culture.