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The dissertation thesis is focused on pelagic fish distribution in the large freshwater bodies and the main factors affecting it. Paper 1 describes fish behaviour in a mouth of a midwater trawl during different day time periods as fish activity may importantly affect abundance estimates of the sampled fish stock. Acoustically recorded avoidance behaviour in a vertical direction is described. The second part of the thesis refers to the diel distribution and behaviour of the pelagic fry communities with a particular focus on the vertically migrating bathypelagic percid fry that occupy open water during early ontogeny. Paper 2 shows that the vertically migrating community can create a dominant part of fry assemblages in the reservoir, which is in contrast to many previous observations of an usually prevailing non-migrating epipelagic community. At their day refuge, bathypelagic percid fry (BPF) created dense shoals whose physical parameters are described. Paper 3 for the first time demonstrates that vertical shifts of BPF were under direct light control, hence were not a genetically fixed behaviour. A unique large-scale field experiment with the simultaneously operating up-looking and down looking transducers was carried out under artificially controlled light regime. Moreover effect of predation as the main ultimate cause of vertical shifts is discussed. The introductory part of the thesis opens with the current possibilities of assessing distribution and behaviour of fish in the open water. Benefits of shoaling/schooling behaviour during defence against predators, foraging and learning abilities of fish are mentioned and some implications of fish behaviour on the fish capture process are pointed out. The second chapter deals with the diel shifts between habitats that belong among the most common activities of fish. However, distribution of fish varies also over the long temporal scale and currently has been strongly affected by changing climate. Therefore, the main affects of climate change on the world´s fish populations are introduced using examples from both freshwater and marine environment.

Annotation Long-term fixed-location hydroacoustic study with uplooking transducer was performed during 2005 in Římov reservoir, Czech Republic. It dealt mainly with fish behaviour in the open water of reservoir, especially with sinusoidal swimming behaviour. The dependence of pelagic fish behaviour on environmental conditions was also studied.

Weakly electric fish produce and detect electric fields and use their electrosensory modality in a number of behaviours including navigation and communication. They can modulate their electric discharge in frequency and amplitude to produce electrocommunication signals in variable patterns during social interactions. In one model neuroethological species, Apteronotus leptorhynchus, the most commonly produced communication signal is the ‘small chirp’ – a brief 10-30ms modulation. Individuals tend to produce these signals at high rates during agonistic interactions. In this thesis I will explore the social value of chirps, and to a lesser extent other communication behaviours, in A. leptorhynchus using a variety of experimental designs involving different staged social contexts. I use time series analysis methods to explore the patterns of chirps produced and accompanying aggressive behaviours. I first characterize electrocommunication and chirping in pairs of free swimming fish and correlate signal production with aggressive displays. Bursts of echoed, or reciprocated, chirps tend to be produced in the intervals separating aggressive attacks. Behavioural analysis shows that fish respond to conspecific chirps with echoed chirps and decreased aggression in social contexts outside the range in which previous modelling and electrophysiological data predicted that chirps could be encoded effectively. I then characterize the chirping and aggressive responses to playbacks simulating intruders with different chirping styles to test whether alternative chirp patterns differentially influence conspecific behaviour. In response to simulated intruders producing chirps that echo the real fish’s chirps with a short latency, less aggressive fish tend to produce more of their chirps in bursts than more aggressive fish. For randomly chirping intruders, the response of fish depends on the rate of chirps delivered. Fish respond less aggressively, with fewer chirps, and echo the stimulus chirps at a higher rate when high rates of random chirps are delivered than when responding to simulated intruders with low rates of randomly delivered chirps. Further, across all playback scenarios, fish that produce chirps in response to the playbacks are more aggressive than those that do not chirp. Finally, to better understand the electrosensory inputs during these interactions, I characterize changes in the electric image received by a restrained fish during movements of a free-swimming conspecific and correlate these with chirp production. When one fish is restrained, bursts of chirps tend to be associated with approach behaviours. Communication signals often function to promote individual assessment of potential rivals during agonistic encounters and bursty, antiphonal chirp exchanges may facilitate these assessments and deter potentially costly physical escalations.

The overall objective of this thesis was to determine selenium (Se) levels in the major compartments of aquatic ecosystems and correlate these data with potential Se effects on early life stages of two native fish species. This work was conducted at two uranium (U) mines located in northern Saskatchewan, Key Lake mine and McClean Lake mine. In addition, a site fidelity study was conducted at Key Lake mine to evaluate movement patterns of northern pike inhabiting lakes receiving effluent discharges.<p> At Key Lake mine, Se was accumulated and biomagnified several orders of magnitude higher than its concentration in water (0.7-2.7 µg/L) in lakes receiving discharges, with Se in prey organisms reaching levels above the proposed 3-11 µg/g dry weight (DW) dietary toxicity threshold for fish. Increased concentrations of Se in aquatic biota led to an increase in the Se concentrations in eggs and tissues of northern pike that rely on these organisms as food sources. Furthermore, increases in the incidence of Se-induced deformities above 30% were recorded in fry originating from adults exposed to high levels of dietary Se (> 3µg/g, DW). The increased frequency of deformities found in northern pike fry was associated with a significant increase in the level of Se in northern pike eggs from exposure sites (31.28 - 48.23 µg/g DW) compared to reference (3.19 ± 0.29 µg/g DW).<p> At McClean Lake mine, Se was accumulated and biomagnified through the aquatic food chain with concentrations in some biota groups (e.g., forage fish) exceeding the lower limit (> 3µg/g DW) of the 3-11µg/g (DW) threshold for dietary Se toxicity in fish. Although both northern pike and white sucker females collected from the exposure site showed greater levels of Se in egg and tissues compared to fish collected from a reference site (likely caused by exposure to elevated levels of Se in prey organisms), no increases in Se-induced deformities were found in the developing fish larvae. The lack of a toxic response in fish larvae is in agreement with Se thresholds for early life stage deformities, with egg Se concentrations in northern pike and white sucker collected at the exposure site below the proposed 10 µg/g (DW) threshold associated with the presence of developmental abnormalities. The applicability of the proposed 7.91 µg/g (whole body, DW) Se toxicity threshold to cold water fish is controversial given that most of the research has focused on warm water fish. Therefore, there is an urgent need to conduct studies that allow us to better understand the environmental fate and effects of Se in north temperate (cold water) aquatic systems. The results of my research will contribute valuable information for the establishment of a realistic and environmentally relevant Se threshold for the protection of fish populations in Canadian waters. During the site fidelity study, fish locations were seasonally and daily recorded using a Lotek SRX_400 receiver with handheld Yagi antenna. The results suggest that tagged pike did not migrate out of the study area throughout the study period, with the mean distance traveled ranging from 50 to 400 m. Differences in movement (distance traveled) and home range were found between reference and exposure sites. Overall data suggest that radio-telemetry is a useful tool in environmental studies. This information on northern pike behavior will be valuable towards developing non-lethal sampling methods that could be applied for assessing the effects of industrial discharges in north temperate aquatic ecosystems.

Weakly electric fish produce and detect electric fields and use their electrosensory modality in a number of behaviours including navigation and communication. They can modulate their electric discharge in frequency and amplitude to produce electrocommunication signals in variable patterns during social interactions. In one model neuroethological species, Apteronotus leptorhynchus, the most commonly produced communication signal is the ‘small chirp’ – a brief 10-30ms modulation. Individuals tend to produce these signals at high rates during agonistic interactions. In this thesis I will explore the social value of chirps, and to a lesser extent other communication behaviours, in A. leptorhynchus using a variety of experimental designs involving different staged social contexts. I use time series analysis methods to explore the patterns of chirps produced and accompanying aggressive behaviours. I first characterize electrocommunication and chirping in pairs of free swimming fish and correlate signal production with aggressive displays. Bursts of echoed, or reciprocated, chirps tend to be produced in the intervals separating aggressive attacks. Behavioural analysis shows that fish respond to conspecific chirps with echoed chirps and decreased aggression in social contexts outside the range in which previous modelling and electrophysiological data predicted that chirps could be encoded effectively. I then characterize the chirping and aggressive responses to playbacks simulating intruders with different chirping styles to test whether alternative chirp patterns differentially influence conspecific behaviour. In response to simulated intruders producing chirps that echo the real fish’s chirps with a short latency, less aggressive fish tend to produce more of their chirps in bursts than more aggressive fish. For randomly chirping intruders, the response of fish depends on the rate of chirps delivered. Fish respond less aggressively, with fewer chirps, and echo the stimulus chirps at a higher rate when high rates of random chirps are delivered than when responding to simulated intruders with low rates of randomly delivered chirps. Further, across all playback scenarios, fish that produce chirps in response to the playbacks are more aggressive than those that do not chirp. Finally, to better understand the electrosensory inputs during these interactions, I characterize changes in the electric image received by a restrained fish during movements of a free-swimming conspecific and correlate these with chirp production. When one fish is restrained, bursts of chirps tend to be associated with approach behaviours. Communication signals often function to promote individual assessment of potential rivals during agonistic encounters and bursty, antiphonal chirp exchanges may facilitate these assessments and deter potentially costly physical escalations.

The overall objective of this thesis was to determine selenium (Se) levels in the major compartments of aquatic ecosystems and correlate these data with potential Se effects on early life stages of two native fish species. This work was conducted at two uranium (U) mines located in northern Saskatchewan, Key Lake mine and McClean Lake mine. In addition, a site fidelity study was conducted at Key Lake mine to evaluate movement patterns of northern pike inhabiting lakes receiving effluent discharges.<p> At Key Lake mine, Se was accumulated and biomagnified several orders of magnitude higher than its concentration in water (0.7-2.7 µg/L) in lakes receiving discharges, with Se in prey organisms reaching levels above the proposed 3-11 µg/g dry weight (DW) dietary toxicity threshold for fish. Increased concentrations of Se in aquatic biota led to an increase in the Se concentrations in eggs and tissues of northern pike that rely on these organisms as food sources. Furthermore, increases in the incidence of Se-induced deformities above 30% were recorded in fry originating from adults exposed to high levels of dietary Se (> 3µg/g, DW). The increased frequency of deformities found in northern pike fry was associated with a significant increase in the level of Se in northern pike eggs from exposure sites (31.28 - 48.23 µg/g DW) compared to reference (3.19 ± 0.29 µg/g DW).<p> At McClean Lake mine, Se was accumulated and biomagnified through the aquatic food chain with concentrations in some biota groups (e.g., forage fish) exceeding the lower limit (> 3µg/g DW) of the 3-11µg/g (DW) threshold for dietary Se toxicity in fish. Although both northern pike and white sucker females collected from the exposure site showed greater levels of Se in egg and tissues compared to fish collected from a reference site (likely caused by exposure to elevated levels of Se in prey organisms), no increases in Se-induced deformities were found in the developing fish larvae. The lack of a toxic response in fish larvae is in agreement with Se thresholds for early life stage deformities, with egg Se concentrations in northern pike and white sucker collected at the exposure site below the proposed 10 µg/g (DW) threshold associated with the presence of developmental abnormalities. The applicability of the proposed 7.91 µg/g (whole body, DW) Se toxicity threshold to cold water fish is controversial given that most of the research has focused on warm water fish. Therefore, there is an urgent need to conduct studies that allow us to better understand the environmental fate and effects of Se in north temperate (cold water) aquatic systems. The results of my research will contribute valuable information for the establishment of a realistic and environmentally relevant Se threshold for the protection of fish populations in Canadian waters. During the site fidelity study, fish locations were seasonally and daily recorded using a Lotek SRX_400 receiver with handheld Yagi antenna. The results suggest that tagged pike did not migrate out of the study area throughout the study period, with the mean distance traveled ranging from 50 to 400 m. Differences in movement (distance traveled) and home range were found between reference and exposure sites. Overall data suggest that radio-telemetry is a useful tool in environmental studies. This information on northern pike behavior will be valuable towards developing non-lethal sampling methods that could be applied for assessing the effects of industrial discharges in north temperate aquatic ecosystems.

Weakly electric fish produce and detect electric fields and use their electrosensory modality in a number of behaviours including navigation and communication. They can modulate their electric discharge in frequency and amplitude to produce electrocommunication signals in variable patterns during social interactions. In one model neuroethological species, Apteronotus leptorhynchus, the most commonly produced communication signal is the ‘small chirp’ – a brief 10-30ms modulation. Individuals tend to produce these signals at high rates during agonistic interactions. In this thesis I will explore the social value of chirps, and to a lesser extent other communication behaviours, in A. leptorhynchus using a variety of experimental designs involving different staged social contexts. I use time series analysis methods to explore the patterns of chirps produced and accompanying aggressive behaviours. I first characterize electrocommunication and chirping in pairs of free swimming fish and correlate signal production with aggressive displays. Bursts of echoed, or reciprocated, chirps tend to be produced in the intervals separating aggressive attacks. Behavioural analysis shows that fish respond to conspecific chirps with echoed chirps and decreased aggression in social contexts outside the range in which previous modelling and electrophysiological data predicted that chirps could be encoded effectively. I then characterize the chirping and aggressive responses to playbacks simulating intruders with different chirping styles to test whether alternative chirp patterns differentially influence conspecific behaviour. In response to simulated intruders producing chirps that echo the real fish’s chirps with a short latency, less aggressive fish tend to produce more of their chirps in bursts than more aggressive fish. For randomly chirping intruders, the response of fish depends on the rate of chirps delivered. Fish respond less aggressively, with fewer chirps, and echo the stimulus chirps at a higher rate when high rates of random chirps are delivered than when responding to simulated intruders with low rates of randomly delivered chirps. Further, across all playback scenarios, fish that produce chirps in response to the playbacks are more aggressive than those that do not chirp. Finally, to better understand the electrosensory inputs during these interactions, I characterize changes in the electric image received by a restrained fish during movements of a free-swimming conspecific and correlate these with chirp production. When one fish is restrained, bursts of chirps tend to be associated with approach behaviours. Communication signals often function to promote individual assessment of potential rivals during agonistic encounters and bursty, antiphonal chirp exchanges may facilitate these assessments and deter potentially costly physical escalations.

Předkládaná práce by měla čtenáře blíže seznámit s chováním ryb, které tito živočichové vykazují vůči vlečným sítím během vzorkování pelagiálu vodních těles. Zaměřuje se na oblast v ústí tralu, kde je spektrum reakcí nejširší. Některé specifické rysy chování jsou uvedeny na příkladech z mezinárodních výzkumů v teoretické části. Jsou zde rovněž přiblíženy základní techniky, používané při studiu chování ryb ve vztahu k vlečným sítím. Praktickou částí je dvouletý výzkum (2009 a 2011) chování ryb na českých údolních nádržích Želivka a Římov ve vztahu k tralovým sítím. Chování ryb v ústí pelagického tralu bylo monitorováno prostřednictvím vertikálního sonaru SIMRAD EK 60 o frekvenci 38 kHz. Předmětem výzkumu bylo studium reakcí jednotlivých ryb se zaměřením na vertikální složku jejich pohybu. Zjišťovali jsme rozdíly mezi denní a noční aktivitou ryb, rychlost a sklon ve vodním sloupci, přímočarost trajektorie pohybu, souvislost mezi velikostí ryb a jejich rychlostí, a v neposlední řadě také vliv abiotických faktorů, které únikové chování podstatně ovlivňují. Znalost tohoto typu chování pomůže vypovědět o selektivitě zařízení, kterou je vhodné znát pro efektivní průzkum zdejších nádrží. Stejně tak užitečné jsou nové informace o vlastním chování ryb, které jsou přínosem nejen pro ekologii.

Weakly electric fish produce and detect electric fields and use their electrosensory modality in a number of behaviours including navigation and communication. They can modulate their electric discharge in frequency and amplitude to produce electrocommunication signals in variable patterns during social interactions. In one model neuroethological species, Apteronotus leptorhynchus, the most commonly produced communication signal is the ‘small chirp’ – a brief 10-30ms modulation. Individuals tend to produce these signals at high rates during agonistic interactions. In this thesis I will explore the social value of chirps, and to a lesser extent other communication behaviours, in A. leptorhynchus using a variety of experimental designs involving different staged social contexts. I use time series analysis methods to explore the patterns of chirps produced and accompanying aggressive behaviours. I first characterize electrocommunication and chirping in pairs of free swimming fish and correlate signal production with aggressive displays. Bursts of echoed, or reciprocated, chirps tend to be produced in the intervals separating aggressive attacks. Behavioural analysis shows that fish respond to conspecific chirps with echoed chirps and decreased aggression in social contexts outside the range in which previous modelling and electrophysiological data predicted that chirps could be encoded effectively. I then characterize the chirping and aggressive responses to playbacks simulating intruders with different chirping styles to test whether alternative chirp patterns differentially influence conspecific behaviour. In response to simulated intruders producing chirps that echo the real fish’s chirps with a short latency, less aggressive fish tend to produce more of their chirps in bursts than more aggressive fish. For randomly chirping intruders, the response of fish depends on the rate of chirps delivered. Fish respond less aggressively, with fewer chirps, and echo the stimulus chirps at a higher rate when high rates of random chirps are delivered than when responding to simulated intruders with low rates of randomly delivered chirps. Further, across all playback scenarios, fish that produce chirps in response to the playbacks are more aggressive than those that do not chirp. Finally, to better understand the electrosensory inputs during these interactions, I characterize changes in the electric image received by a restrained fish during movements of a free-swimming conspecific and correlate these with chirp production. When one fish is restrained, bursts of chirps tend to be associated with approach behaviours. Communication signals often function to promote individual assessment of potential rivals during agonistic encounters and bursty, antiphonal chirp exchanges may facilitate these assessments and deter potentially costly physical escalations.