Juvenile sockeye, coho, and chum salmon and adult goldfish were studied for a period of ten weeks to determine whether varying degrees of salinity influenced their growth. The possible influences of such factors as temperature and food were rigidly controlled. Coho and chum salmon showed higher percent weight increase in the saline media. Coho grew best in 12% salinity and chum had a higher percent increase in weight in 30% salinity. The growth of sockeye in the saline medium was retarded for the first eight weeks, but during the last two weeks it surpassed that of the corresponding group of sockeye in fresh water. The early retardation in growth of sockeye, in the saline medium, is attributed to its longer fresh water life. The adult goldfish did not show any significant difference in weight increase. The records of the sizes attained by several species of fish inhabiting both sea and fresh waters show that salinity enhances growth. The evidence from experimental study, by other workers, on the influence of different environmental factors on growth of fishes, indicates that changes in meristic counts or body proportions, in early development, produces different growth rates. These changes could eventually affect the ultimate size. The physiological mechanisms of growth of fishes are not well understood, but it has been suggested that the influence of hormones on growth is probably ameliorated in the marine environment. / Science, Faculty of / Zoology, Department of / Graduate

Based on ethnographic fieldwork in the traditional commercial linefishery on South Africa’s southern Cape coast, informed by social-ecological systems (SES) thinking, and directed by a participatory action research approach, the work facilitated the co-development of a series of applied responses to local challenges identified by research participants including commercial linefishers, school learners, teachers, and other local community members. The thesis is presented in four chapters, each focussing on a different challenge: marine water temperatures; school learning for social learning; fishers’ organisations; and branding of linefish. The objectives of the thesis are to explore the processes, constraints, motivators, and lessons learnt in addressing each of the four challenges drawing on four emergent themes: 1) trust and social capital, 2) social learning, 3) resilience and transformation, and 4) participatory action research/co-development. The thesis underlines how participation leads to co-developed strategies to address realworld challenges. The work on water temperatures resulted in the co-development of a novel water temperature measuring device for deployment on commercial linefishing boats. However, despite initial successful deployment, fishers’ time and financial concerns, paired with a short-term focus undermined the participatory process. The social learning and teaching work facilitated the co-development of a series of integrated teaching modules that addressed challenges observed in the school, transforming the approach to teaching, and laying the foundation for future community social learning. The work also raised the challenge of ‘high stakes testing’ which may constrain teacher participation. The work on fishers’ organisations revealed the role of leadership, competing economic and lifestyle foci, competence and political trust, as well as ‘bonding’, ‘bridging’, and ‘linking’ forms of social capital in the formation, maintenance and dissolution of these bodies. The research found that different forms of trust and social capital, paired with leadership, were critical to successful participation and collaboration throughout the fieldwork. Finally, the branding work resulted in an adaptation on the part of the fishers, but one which was constrained by and highlighted the economic influence of inshore trawling that continues to limit the extent of linefishers’ adaptive strategies.

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No description available.

In the aquaculture of fish and shrimps, diets generally contain high levels of fish meal. Fish meal is a nutrient rich feed that contains essential amino acids and fatty acids, which are required to maintain optimum growth and health of an animal. It is traditionally used in aquaculture to promote feed efficiency, nutrient uptake and feed intake due to its high palatability and digestibility. Overexploitation of fisheries resources and the high growth rate of aquaculture industry, however, continues to put pressure on the supply of fish meal, thus increasing the demand and price of this sought-after product. The use of alternative ingredients to fish meal, therefore, remains a high priority for aquaculture nutrition. Hence, the aim of this was to investigate the physiological and developmental effects on juvenile spotted grunter, Pomadasys commersonnii, after supplementing fish meal with different feed additives for 12 weeks. One of the additives was then selected for further feeding at graded levels (increasing levels) for 8 weeks to ascertain whether fish meal could be replaced by higher amounts of the feed additive without negative consequences to the cultured spotted grunter.

Accurate interpretation of recent variability observed in fish populations, species compositions, distribution ranges or ecological indicators, depends on knowledge of their past dynamics and historical states. The onset of systematic fisheries data collection typically lagged decades or even centuries behind modern fishing exploits. As a consequence, pre-disturbed reference points and descriptions of subsequent change are rarely available. A remarkably detailed set of historical trawl survey data from South Africa provided such a rare opportunity. Government-funded exploration of the Agulhas Bank fishery potential resulted in meticulously-documented trawl survey data from 1897-1904, when prior human impacts on those resources were negligible. Although they used less effective technology, the information recorded and methods used were similar to modern surveys. This thesis investigated change in demersal fish fauna of the Agulhas Bank and documents comparisons between historical trawl surveys and modern re-enactments at the same locations. In comparing trawl survey catches over multiple decades or among different periods, unquantified changes in fishing power pose a key challenge. The shape, size, materials, mesh sizes and speed at which trawl nets are dragged, interact with the behaviour, size and shape of fish, influencing fishing performance. To accurately compare current catch rates with those of historical trawl surveys, the same trawl gear and methods were carefully replicated in repeat surveys at three sites. An investigation of literature and photographs of the original vessel and equipment were conducted to support the construction of a replica 'Granton' otter trawl net. The net was composed of Manila hemp with a headline length of 27 m (90 ft) and was attached to flat wooden trawl boards. The historical towing speed was estimated as 1.34 m s⁻¹ (2.6 knots). Three parts of the shallow Agulhas Bank that were surveyed 111 years prior, were re-surveyed in 2015. Species composition was contrasted between the historical and re-survey periods by way of unconstrained ordination, permutational multivariate analysis of variance and tests of the homogeneity of multivariate group dispersions. Taxa discerning between periods were identified with similarity percentage analyses. Changes of standardised catch between periods were tested for 27 taxa, using a non-parametric bootstrap approach. Proportions among size-classes, recorded for three taxa, were tested using Fisher's exact test. Results revealed a substantially transformed demersal catch assemblage, where the period effect explained almost half of the measured variance among samples. These changes included the disappearance or heavy depletion of kob (Argyrosomus spp., absent in re-surveys), panga (Pterogymnus laniarius; 2.4% of historical catch abundance) and east coast sole (Austroglossus pectoralis; 4.6% of historical catch abundance), which had jointly contributed 70-84% of historical catch composition. Average re-survey catches were largely made up of gurnards (Chelidonichthys spp.; 3 792% of historical abundance), horse mackerel (Trachurus trachurus; 4 738% of historical abundance), spiny dogfish (Squalus spp.; 3 121% of historical abundance), hake (Merluccius capensis; 558% of historical abundance) and white sea catfish (Galeichthys feliceps; 13 863% of historical abundance). Analysis of available length information confirmed the expectation that fish sizes (specifically M. capensis and A. pectoralis) had declined. This implies that comparisons by weight would be more severe for declined abundances and less severe for those that increased, relative to contrasts of numerical abundance. Habitat preferences as well as geographic and depth distribution appeared to separate the taxa that increased from those that declined. These factors, together with reproductive and growth characteristics, as well as indirect trophic impacts, likely shaped the responses of demersal fauna to fishing and other human impacts during the 111 years between trawl surveys. An assessment of distribution changes of 44 common demersal taxa was undertaken. These analyses were restricted to the last 30 years of trawl survey data as the units and spatio-temporal resolution of prior data were incompatible. Standardised catches were used from annual spring and autumn south coast trawl surveys conducted by the government fisheries department. Geostatistical delta-generalised linear mixed models were used to predict species distribution functions, which were used to calculate annual estimates of latitude/longitude centres of gravity and effective areas occupied by each population. Average trends over the study period (1986-2016) were assessed using a Bayesian state-space model. Of nine species found to have a trend in average location, six moved westward or south-westward, while three moved eastward or north-eastward. Two species showed a trend of contracting spatial extent and one showed an expansion. Across the entire assemblage combined, there was a significant contraction in extent and a westward shift in average location. These assemblage-wide average trends are interpreted to be driven by climate forcing. Fishing impacts are expected to have contributed to the eastward movement in centre of gravity for kob, lesser sandshark (Rhinobatos annulatus) and white stumpnose (Rhabdosargus globiceps). Interpretation of these distribution shifts is hampered by a lack of knowledge on subsurface hydrographic trends on the Agulhas Bank, which is identified as a research priority. My research revealed substantial change in demersal fish communities on South Africa's Agulhas Bank and adds novel insight to the history underlying current states of demersal ecosystems. Valuable additions include a) documentation of the extent to which demersal assemblages have transformed during the last century at three representative inshore sites; b) estimates of current abundances relative to pre-disturbed baselines at those sites, which c) highlights drastic local depletion for certain taxa and d) reveals substantial abundance increases of certain species during the post-industrial fishing period; e) novel evidence of distribution trends in south coast demersal species; and f) identification of trends in the average distribution of the demersal fish assemblage, suspected to be climate-forced. Long-term comparisons, using minimally-disturbed baselines, revealed drastic transformation of the fish assemblage during a century of industrialisation, which points to trawling-induced alteration of benthic habitats and substantial changes in ecosystem structure. Besides the provision of novel historical context for current and future studies and decision-making, this work counters the erosive nature of shifting baselines in South Africa's marine environment.

An understanding of the population structure of commercially exploited species is essential for effective fisheries management. Fish stocks are typically identified based on the observation of differences in genetic and/or phenotypic characteristics between fish from discrete units. Recent deliberations on the management of the South African purse-seine sardine fishery recognises the likely existence of two stocks, one on the west and another on the south coasts, following studies that have documented spatial variability in several phenotypic characteristics of this species around South Africa. Those studies typically examined spatial variability in a single characteristic (e.g. gill raker morphology and meristics, body shape, otolith shape, vertebral count, parasite loads), but the application of multiple stock identification methods to the same individual fish has been recommended in order to maximize the likelihood of correctly inferring and identifying fish stocks. This study seeks to assess the population structure of South African sardine Sardinops sagax using a combination of stock identification methods including meristic, morphometric and parasite markers. Most of the sardine samples used in this study were obtained off the west and south coasts of South Africa during pelagic surveys conducted in 2013 and 2014 by Fisheries Branch of the Department of Agriculture, Forestry and Fisheries, with some additional samples obtained from commercial fisheries. Fish caught from the west and south coasts are presumed to be part of the putative western and southern stocks respectively. The variables examined included body shape, gill arch length, gill raker spacing, number of gill rakers, otolith shape indices (otolith circularity and form factor), the number of vertebrae, and the abundance of a 'tetracotyle' type metacercarian parasite found in the eyes of sardine; these were first analysed individually and then collectively (excluding body shape data) in a multivariate analysis to test their effectiveness in discriminating between sardine from the putative western and southern sardine stocks. Analysis using geometric morphometrics revealed significant differences in body shape between sardine from the putative western and southern stocks. Results of GLM analyses indicated that gill arch length, the number of gill rakers, otolith form factor and circularity, and parasite abundance were effective univariate discriminators of sardine stocks. No significant difference in gill raker spacing and the number of vertebrae was found between individuals from the two stocks. A Stock Identification Index (SDI) of 0.75 derived from the univariate analyses, as well as results of the multivariate analysis of data provided strong evidence for the existence of two mixing stocks, therefore, supporting the two-stock hypothesis. The five multivariate classification models used in the study showed varying degree of allocation success. Overall classification accuracy ranged from a low 47% in the Linear Discriminant Analysis model to highs of 82% and 91% in the Classification Tree Analysis and Random Forest models, respectively. Both CTA and RF revealed the combination of variables with the strongest spatial discriminatory power to be the number of vertebrae and abundance of the 'tetracotyle' type metacercarian parasite. These results agree with those of previous studies and further support the inclusion of sardine population structure into management strategies for the purse-seine fishery for South African sardine. Given the importance of the 'tetracotyle' type metacercaria in the discrimination of South African western and southern sardine stocks, further studies were undertaken to identify and describe this parasite using light and scanning microscopy, as well as molecular tools. Such information will assist in the definitive identification of the first intermediate host of the parasite, presently hypothesized to be a sub-tidal gastropod occurring off the west coast only. Definitive identification of the first intermediate host (or hosts) will enable the full life cycle of this parasite to be determined and the parasite endemic area to be identified; a critical knowledge gap in application of the parasite biotag approach to stock discrimination using this 'tetracotyle' type metacercarian parasite. Metacercariae collected from fresh sardine sampled from a commercial landing in Gans Baai, South Africa, were manually excysted, relaxed in warm water, fixed in 70% ethanol and stained with haematoxylin for light microscopy. The metacercarial body is oval-shaped, measuring 762 - 967 x 512 - 677μm. It is divided by transverse folds into a forebody, midbody and hindbody. Diagnostic features include the unique, large excretory bladder lobes situated on the lateral sides of the body; two large pseudosuckers in the anterior part of the midbody; an acetabulum which is larger than the oral sucker; and a large lobulated holdfast organ in the posterior half of the midbody. These features are those of the metacercariae of the genus Cardiocephaloides, confirming the previous hypothesis regarding the genus of this digenean biotag. Analysis of partial 28S rDNA region sequence data showed that the metacercariae and the adult Cardiocephaloides found in the African penguin Spheniscus demersus are of the same species, likely C. physalis. This study is the first documentation of the morphological and molecular characterization of the stock-discriminating Cardiocephaloides metacercaria found in the eyes of Sardinops sagax in South Africa.

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