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M.Sc. (Aquatic Health) / The lower Olifants River within the Kruger National Park is regarded as an environmentally sensitive area which has been found to exhibit high levels of aquatic diversity. The biodiversity in this lower section of the Olifants River is under threat as a result of the cumulative impacts upstream within the catchment. These impacts are apparent in water pollution, siltation and reduced stream flows as a result of agriculture, mining, industry and power generation. Although the impacts within the upper catchment have been well documented, it was the numerous fish kills within the river which brought the issue of river health into the public eye. Due to the intensive industrial activities on the Ga-Selati River, a tributary of the Olifants River, and its close proximity to the Kruger National Park, much focus has been placed on the river’s water quality, especially industrial effluent containing high concentrations of pollutants. Currently bi-annual monitoring of the aquatic ecosystems associated with the industries along the Ga-Selati River is being conducted, providing information of the ichthyofaunal communities within the system. In addition to this, the separate industries collect water quality data as frequently as weekly. The aim of this project is to lay the foundation for understanding the state of the Ga-Selati River, and to determine what water quality variables may be influencing the ichthyofaunal structure within the river. Analysis of water quality data showed that elevated levels of salts are evident, with a downstream increase in concentrations being observed along the length of the Ga-Selati River at a given point in time. Over the eight years of data considered, the overall trend indicates a general water quality improvement (decreased concentrations). A reoccurring trend showed an increase in salts between two of the sites, suggesting a source of contamination within this area. A statistical analysis of the fish community data and environmental data showed there to be a clear distinction between historical (2003, 2004 and 2005) and recent surveys (2009 and 2010). During the first time period, Site 1 showed the lowest diversity and showed to be significantly different from the remainder of the sites. During the second time period, diversity at Site 1 increased, and it was noted that overall, there was greater variability within the data. In general it was observed that upstream species richness was lower compared with downstream sites. This trend is likely linked to the proximity of the Olifants River, which allows migration into the lower reaches of the Ga-Selati River. The historical monitoring data revealed that water quality and in particular salts are influential in the structuring of ichthyofaunal communities. This change can be substantiated by identifying that an improvement in water quality has resulted in a positive shift in ichthyofaunal community structure. The analysis of this information will contribute towards the improved management and conservation of the Ga-Selati River system. Although mining houses/industries are collecting and sharing water quality data, it would be valuable if a strategic approach to sample collection and management was perused. A centralised database will increase the understanding of the driving variables behind ichthyofaunal community structure within the river and would lay the foundation for future closure plans. It is imperative that an excellent set of water quality data is available during the design of appropriate rehabilitation and treatment facilities.

Mean salinity concentrations in the four reservoirs (Moss, Ray Hubbard, Texoma and Possum Kingdom) ranged from 0.2 ppt in Moss Lake to 2.01 ppt in Possum Kingdom Lake. Reservoir sodium and chloride concentrations were hypotonic to hypertonic to plasma levels in all species. Interspecific differences were seen in ionic concentrations within each reservoir. Total osmotic and sodium concentrations in carp, Cyprinus carpio, were correlated to their concentrations in the reservoirs. No such relationship was noted for chloride, potassium and calcium. A laboratory study indicated that fish collection by electroshock did not bias plasma ion concentrations. Exposures to wide variations in ionic concentrations did not appear to induce stress in the species studied.

Thesis (M.Sc. (Zoology)) --University Limpopo, 2013 / Major anthropogenic activities such as, mining, coal-fired power stations and intensive agricultural practices in the upper catchment area of the Olifants River, have a dramatic impact on the water quality downstream. As a result the river is presently the third most polluted river in South Africa. The aim of this study was to assess the impact of water and sediment quality on the health of Labeo rosae and Schilbe intermedius. The aim was accomplished by applying the Fish Health Assessment Index (HAI) which includes the Parasite Index (PI) at Flag Boshielo Dam in the Middle Olifants River, Limpopo Province. This was achieved through; assessing the water quality of the dam by determining the levels of physical and chemical constituents in the water at three sampling sites, determining the bioaccumulation of selected metals in the muscle tissue of the two fish species, assessing the fish health (including the fish condition factor) and the fish parasites in the dam by using the fish HAI and PI, and ascertaining the Human Health risk factor upon consumption of fish contaminated with metals from the dam. The water and sediment quality were seasonally sampled at three sites in the dam: inflow, middle and wall. Dorsal muscle tissues from both fish species were collected for metal bioaccumulation analyses. The water, sediment and fish muscle tissue samples were analysed by an accredited laboratory by means of ICP-OES spectrometry. For the fish health and parasites, ten fish per species were collected seasonally (July 2009 to April 2010) by means of gill nets and examined at a field laboratory using the HAI and PI protocol. Generally the water quality of Flag Boshielo Dam was acceptable for aquatic ecosystems according to the SAWQG with the exception of phosphorus and some metals at the inflow area. The pH ranged between slightly acidic to alkaline values; water temperature: 15°C to 26°C; water hardness medium soft, salinity within the freshwater range; turbidity in the clear water range. The TDS and major ions (salts) were acceptable for the duration of the study. The highest concentrations of nutrients (specifically phosphorus) as well as metals (aluminium, cadmium, copper, iron and lead) were recorded at the inflow area of the dam. The nutrients were very low except the eutrophic range phosphorus concentrations recorded at the inflow whereby, the Elands River may be an additional source of nutrients into Flag Boshielo Dam. The metals that were recorded above TWQR are; aluminium, cadmium, copper, iron and lead, of which were mostly recorded at the inflow. However, statistically there was no significant difference among the three sampling sites. The metal concentrations at the inflow were recorded only slightly higher than the middle and the dam wall. The main source of the metals may be the water coming from catchment area of the dam given the intensive agricultural activities taking place between Loskop Dam and Flag Boshielo Dam. Sediment and bioaccumulation: All the metals were recorded at higher concentrations in the sediment than in the water and fish muscle tissue, except antimony, selenium and strontium. The most abundant metals recorded in the sediment were iron and aluminium. However, the concentrations above the TEL were cadmium, nickel and zinc. The elevated metal concentrations in the sediment are indicating that the metal load in the sediment of Flag Boshielo Dam could be a potential risk for the aquatic biota if they become bioavailable. Antimony, selenium and strontium metal concentrations were recorded at higher concentrations in the muscle tissue of both fish species than in the sediment and water. Iron was the most accumulated metal in the muscle tissue of both fish species. In terms of numbers, more metals were recorded in the muscle tissue of S. intermedius than in L. rosae however the metal concentrations were higher in the latter. This can be attributed to their different trophic levels in the food chain; L. rosae is a primary consumer while S. intermedius is a tertiary consumer. However, the metals that accumulated in the fish muscle tissue were indicative of bio-availability of the toxic metals in the dam and not water/sediment pollution. According to a Human Health risk assessment (Chapter 3), metals that may have risks upon consumption of L. rosae are; antimony, arsenic, chromium, iron and vanadium; for S. intermedius are; antimony, chromium, iron, vanadium and arsenic (in descending order). These metals may pose toxic and carcinogenic risks to humans. Therefore, the rednose labeo (L. rosae) and to a lesser extend the silver catfish (S. intermedius) fish species from Flag Boshielo Dam may not be suitable for humans if consumed above 350 g per week. Fish health and parasites: The Health Assessment Index (HAI) values of the two fish species differed significantly with higher index values recorded for S. intermedius than L. rosae. Besides the Parasite Index (PI), abnormal haematocrit readings, liver conditions, skin lesions and clubbed gills are the necropsy anomalies that contributed predominantly to the HAI. Overall, the PI contributed mostly to the total HAI value. The parasite load and therefore also the mean intensity, mean abundance and prevalence of S. intermedius were higher during all seasons than that of L. rosae. The dominant ectoparasites for both species were from the Class Monogenea and the dominant endoparasites were nematodes. Out of 40 L. rosae sampled, 139 parasites were retrieved; five parasite species were ectoparasites and two endoparasites. From the 40 S. intermedius sampled, 2473 parasites were retrieved, from which two species (one genus) were ectoparasites and three species were endoparasites. The condition factor is used to compare the “condition”, “fatness” or wellbeing of fish and it is based on the hypothesis that heavier fish of a particular length are in a better physiological condition. The L. rosae had a better condition factor, recorded at values less than (2) as compared to S. intermedius (>2). The cause of the HAI necropsy anomalies may have been also from parasite load other than the metals in the water and sediment. However, the HAI alone cannot be used for metal pollution, unless it is done in conjunction with a histopathological study of the tissues/organs. Therefore, the cause of the recorded anomalies from both fish species is inconclusive. On the hand, fish can be used as bioindicators because the accumulated metals in the fish tissues are indicative of the bioavailability of metals in Flag Boshielo Dam.

Temperature, conductivity, and Aeromonas hydrophila density were determined bimonthly at six sites in the White River drainage system, Delaware County, Indiana, from April, 1984 through December, 1984. Fish were collected from four of the six sites. Fish were identified to species, examined for gross pathology, and their blood collected. Titers against A. hydrophila antigen 157 were determined for all carp (Cyprinus carpio) captured. Certain carp serum samples were also tested against three additional A. hydrophila antigens.Aerononas hydrophila densities appeared to be related to temperature and conductivity, primary producers, and runoff/effluent. Aeromonas hydrophila densities were higher in areas affected by urban runoff/ effluent than in areas affected by rural runoff/effluent. Aeromonas hydrophila densities increased as one progressed through the city of Muncie.Only five percent of all fish captured exhibited signs of gross pathology. Diseased fish typically demonstrated low titers against antigen 157. Several A. hydrophila serotypes were apparently infectious for the carp studied. Thirty-two percent of the carp sampled exhibited a positive titer response against antigen 157. Considerable variation in titer response existed between individuals.Ball State UniversityMuncie, IN 47306

M.Sc. (Zoology) / Please refer to full text to view abstract

Thesis (M.Sc. (Zoology)) -- University of Limpopo, 2020 / The present study was carried out in the Kwena Dam, with the aim of determining selected water quality parameters, parasite diversity and condition factor (K) of Clarias gariepinus (sharptooth catfish), Cyprinus carpio (common carp) and Oreochromis mossambicus (Mozambique tilapia). This study was conducted in autumn (April 2016), winter (July 2016), spring (October 2016) and summer (February 2017). The present study was the first to investigate the parasite composition of these three fish species in the Kwena Dam. A total number of 26 Clarias gariepinus, 21 Cyprinus carpio and 57 O. mossambicus specimens were collected using gill nets of different mesh sizes (30 mm – 120 mm). Each fish was weighed, measured and euthanised by severing the spinal cord. Mucus smears from the skin, fins and gills were examined for ectoparasites using a stereo-microscope. The fish were then dissected and all organs examined for endoparasites. All parasites were fixed and preserved according to standard methods for each parasite group. In situ water parameters were determined using a handheld multi-parameter instrument for each sampling season. In addition, water samples were collected seasonally and sent to an accredited laboratory where they were analysed for selected metals and nutrients. Water quality parameters and the presence of metals in water are of importance in determining the water quality of an aquatic environment. Most water quality parameters were within the Target Water Quality Range (TWQR) for aquatic ecosystems. Aluminium, selenium and zinc had concentrations above the TWQR for aquatic ecosystems. Nutrient concentrations were within the TQWR during all sampling seasons. The water quality did not differ significantly between seasons during the present study. Four parasite groups were reported infecting Cyprinus carpio and these included Monogenea (Dactylogyrus extensus and Dactylogyrus minutus), Digenea (Diplostomum sp.), Cestoda (Atractolytocestus huronensis), Branchiura (Argulus japonicus) and Copepoda (Neoergasilus japonicus). Parasites collected from Clarias gariepinus belonged to four groups, namely Protozoa (Trypanosoma sp.), Monogenea (Quadriacanthus sp. and Gyrodactylus sp.), Nematoda (Paracamallanus cyathopharynx and Contracaecum sp.) and Branchiura (Dolops ranarum). Parasites collected from O. mossambicus belonged to five groups, namely Monogenea (Cichlidogyrus halli, Cichlidogyrus sclerosus, Cichlidogyrus tilapiae and Enterogyrus conoratus), Nematoda (Contracaecum sp.), Cestoda (Neogryporhynchus sp.), Acanthocephala (Acanthogyrus tilapiae) and Branchiura (Dolops ranarum). iv The number of parasite species for the four seasons were as follows: summer (13) > autumn and winter (12) > spring (11). From the Shannon-Wiener index results, O. mossambicus had a higher parasite diversity than Clarias gariepinus and Cyprinus carpio. The Parasite Index (IP) and Inverted Parasite Index (IPI) of the three fish species indicated that the water from the dam is not polluted. The condition factor (K) for all fish species indicated that fish collected from the dam during all sampling seasons were in a good condition and parasite load had little effect on K for all fish species. The use of PI and IPI in conjunction with the fish K can be regarded as a useful tool in freshwater and fish health monitoring. The present results report new geographical records of the parasites of Clarias gariepinus, Cyprinus carpio and Oreochromis mossambicus. Since no parasitological research was done before the present study at the Kwena Dam, the results of the present study form baseline data for future parasitology studies and can consequently be useful in the management and conservation of the Kwena Dam. / DSI–NRF SARChI Chair (Ecosystem Health) of the University of Limpopo and the Limpopo Department of Economic Development and Tourism (LEDET)

Thesis (M.Sc. (Zoology)) -- University of Limpopo, 2018 / This study aimed to determine the effects of water and sediment quality on fish and the effects of land-use on the water and sediment quality of the Steelpoort River system. To achieve these aims, a few objectives had to be met. The water and sediment quality, the level of metal bioaccumulation of two fish species, the level of acetylcholine esterase inhibition in fish brain tissue, as well as changes in land-use over time. The study adds value in the scientific knowledge of the Steelpoort catchment given that there’s little information about it. It is thus a good baseline, which gives direction for further studies conducted within this catchment. The Steelpoort River, is a tributary of the Olifants River system. The Olifants River (Mpumalanga and Limpopo provinces) is a river stressed by anthropogenic activities with the Steelpoort River being a major sub-catchment. The Steelpoort catchment is filled with mines, agricultural activities, and other developments, such as, the construction of a new dam (De Hoop Dam opened 2014), residential areas and malls. The mines within the catchment are mostly platinum and chrome mines. Sampling was conducted for high and low flow at five sampling sites: Two upstream sites, 1 and 2, and three downstream sites 3, 4, and 5. Site 1 is the reference site for the study as this section (ecoregion) was described as “least threatened”. It was hypothesised that high concentrations of metals and nutrients would be present in the water and sediment, and that the metal would be present in fish tissue and would have an effect on human health, that the biomarker AChE would show signs of inhibition within brain tissue, and lastly that land-use characteristics would have an effect on the water and sediment quality, and inhibition of AChE. Water and sediment samples were collected quarterly over a period of a year at five sites, whereas fish samples (Chiloglanis pretoriae and Labeobarbus marequensis) were only collected bi-annually at three sites. The physico-chemical, nutrients, cations and ions, and metals and metalloids were determined. The two fish species were used for metal bioaccumulation and AChE analyses. Human health risk and bio-concentration factor (BCF) analyses were conducted. Using the land-cover details obtained from South African National Biodiversity Institute (SANBI), Department of Environmental Affairs (DEA) and The Council for Scientific and Industrial Research (CSIR), land-use characteristics were determined and evaluated. The in-situ physico-chemical parameters assessed in this study were found to be within acceptable limits of the Target Water Quality Range (TWQR) at all the sites. The Electrical Confuctivity (EC) and Total Disolved Solids (TDS) parameters were highest at Site 5 and lowest at Site 1, this could be due to Site 5 being the last site before the Steelpoort River connects to the Olifants River, hence this site recieves all the ions and solutes from upstream; and the lowest value at Site 1 could be attributed to this site having the least number of lan-use effects. For nutrients, the highest total nitrogen concentration was recorded at Site 5 and the highest phosphate concentration at Site. This river is mesotrophic according to both the total inorganic nitrogen and phosphorus levels. Metals in the water column that were above DWAF guidelines for Aquatic Ecosystems were Al, Ba, Mn and Zn. In the sediment the following metals were above the Canadian sediment quality guidelines: Cd, Cu, Cr and Zn, which can pose a threat to the health of fish through biomagnification through the food chain. Both C. pretoriae and L. marequensis had bioaccumulated metals from the river system. The concentrations of metals in the muscle tissue of C. pretoriae where higher and this could be due to the fact that unskinned samples were used in the case of C. pretoriae. A variety of metals were present in both fish species but Al, Fe, Sr and Zn were present in higher concentrations. Concentrations of metals were highest in samples from Site 5 The human health risk assessment revealed that Pb could cause health related impacts to the humans who consume a single 150 g fish meal once a week in C. pretoriae; and that Co could cause potential risks in the near future. The BCF values revealed that there were more bioconcentrated metals in C. pretoriae than in L. marequensis and this could also be due to different diets. The AChE activity was higher for L. marequensis during high flow at all sites and higher at Site 1 during low flow but lower at Site 5 and equivalent at Site 2 than the AChE activity recorded for C. pretoriae. This could be due to the ability of L. marequensis to expel pesticides from their system before the pesticides affect brain activity by decreasing the function of AChE. Both fish species had more AChE activity during low flow for sites 1 and 2 than during high flow. The high flow AChE activity was higher for Site 5 in both fish species. The results confirm the hypotheses were that metals would be present in fish tissue and would have an effect on human health; and that the biomarker AChE would show signs of inhibition within brain tissue. The agricultural activity has decreased over time within the catchment, from 2000 to 2014 and this could be due to a decrease of fertile land. There has also been a decrease in the mining activity from 2000 to 2009 and then an increase during 2014, where the decrease could be due to the closure of mines at the end of their life span; and the increase in 2014 could be due to the opening of new mines. Residential areas have increased from 2000 through to 2014. The availability of water decreased from 2000 to 2009, and then increased drastically in 2014. The drastic increase is due to the multiple dams that have been built over time in order to provide water resources to the increased population, and also for mining and irrigation purposes. The water quality showed an increase in the NO3, SO4 and PO4; meanwhile the EC has decreased over time. The decrease in EC means that there are fewer ions in the river. The increase in phosphates and nitrates could mean there is a lot of fertilizers and pesticides being used in agriculture even with the decrease in agricultural land-use over the years. The pesticides can also be due to the increase in residential areas, where pesticides are also used to get rid of pests.

M.Sc. (Aquatic Health) / Aquatic ecosystems in rural South Africa have been impacted over many years by commercial and subsistence anthropogenic activities (DWAF, 2001). These impacts include commercial and subsistence farming, domestic use (e.g. washing of clothes, cars and bathing) and recreational use (e.g. fishing). In the northern parts of the Limpopo Province of South Africa (Vhembe District) the land use is primarily dominated by various agricultural activities and human settlements. Rivers in this region flow through mixed agricultural use, including commercial agriculture (tea and timber plantations) as well as subsistence farming and housing. Perennial rivers in this region include the Mutale, Mutshindudi and Tshinane rivers. These rivers are located in the Water Management Area 2 (WMA 2 Luvuvhu-Letaba) (Kleynhans, et al., 2007a). They are highland river systems, originating in the nearby Soutpansberg Mountain, and these rivers all form part of the larger drainage system of the Luvuvhu River, a tributary of the Limpopo River. The wetlands in the catchment of these rivers are also heavily utilised by the local communities (Working on Wetlands, 2013) used for grazing, sand mining and subsistence cultivation, thus providing a livelihood for the surrounding communities (SANBI, 2012). The aim of the study was to assess aspects of the biological integrity of the Mutale, Mutshindudi and Tshinane rivers. This study formed part of a larger study that included assessments of both abiotic and biotic aspects of these river systems. For this study, the focus was on assessing the fish community structure through the application of Fish Response Assessment Index (FRAI), riparian vegetation through the application of Vegetation Response Assessment Index (VEGRAI) and fish health aspects with special reference to liver and gonadal histopathology. Water quality parameters were found to be within the target water quality range for aquatic ecosystems. The results did however vary between upstream and downstream sampling sites. Similarly, compared to upstream sites, the FRAI showed the Mutale River to improve in fish community structure whereas the Tshinane and Mutshindudi rivers showed a decrease in the FRAI score. The VEGRAI results showed a decrease in EcoCondition in the Mutale and Mutshindudi rivers whereas the Tshinane indicated an increase. This can be attributed to land use change up to the edge of the river systems. Visual observation at the various study sites showed a definite localised impact of human activities on the beds and banks of many parts of the rivers. No histological alterations were identified in any of the gonadal or liver tissue of the Chiloglanis pretoriae (Shortspine suckermouth) (Van der Horst, 1931) and the fish were found to be in a healthy condition according to the selected parameters (gonads and livers) assessed. The fish community structure of the three rivers were found to be in a moderately to largely modified condition according to the ecological state categories calculated. However, the fish health assessment showed no histological alterations in the sampled fish. It is proposed that future studies investigate the influence of the domestic use of the rivers as well as attempt to quantify the impact of agriculture on the system.

Turbidity is an important measurement of water quality, considering it describes water clarity and is an indirect indicator of light transmittance in the water column. Turbidity may impact fish that rely on vision to forage by affecting ability to search for prey. Largemouth bass (LMB; Micropterus salmoides) is a visual predator that may have lower foraging success resulting in reduced growth, reproduction, or survival under conditions of high turbidity. I conducted a field experiment in Wolf Lake, Mississippi to assess impacts of turbidity on diet and distribution and a laboratory experiment with manipulated turbidity levels (0 – 250 NTU) to assess foraging efficiency of LMB. There were no significant relationships between turbidity and diet or distribution in the field study. However, there were significant effects of turbidity on foraging efficiency in laboratory trials. These results indicate that assessing turbidity is important to manage LMB in systems susceptible to high turbidity levels.