The first comprehensive phylogeny of the family Curtonotidae is presented based on molecular markers and morphology. This enabled assessment of evolutionary relatedness, species radiation and zoogeography. A revised classification of the world fauna of the family, based on the results of this study is outlined. Nomenclatural acts flowing from these analyses include: the recognition three new Afrotropical species of Axinota van der Wulp; four new species of a new genus, Tigrisomyia Kirk-Spriggs; and nineteen new species of Curtonotum (six Madagascan and thirteen African). Ten informal species-groups of Curtonotum are recognised. Identification keys are provided for the Afrotropical species of these genera and errors in previous interpretations and designation of type specimens are resolved. Species distributions are mapped and interpreted and discussion is made of the historical biogeographical significance of these distributions.

The persistance of Escherichia coli in the larval, pupal and adult stages of both house flies, Musca domestica (L.), and stable flies, Stomoxys calcitrans (L). was examined. Abundance of E.coli declined over time in immature house flies, but remained constant in immature stable flies, suggesting house fly larvae digest E. coli but stable fly larvae do not. Survival of house fly and stable fly larvae averaged 62% and 25% respectively when reared on pure E. coli cultures. E. coli load in pupae decreased significantly one day before emergence of adult house flies, but remained constant until stable fly emergence. Nevertheless, E. coli was detected in 78% of emerging house flies and in 28% of emerging stable flies. House flies are more important E. coli vectors as adults, whereas stable flies may be overlooked vectors of E. coli during immature development. / ix, 89 leaves ; 28 cm.