The Florida mouse (Podomys floridanus), a species restricted to the Lake Wales Ridge and the Atlantic Coastal Ridge, is recognized by the Florida Fish and Wildlife Conservation Commission as a species of special concern, highlighting its status as a species that is threatened by habitat loss statewide. Publicly owned lands offer protection for the species, but management is generally focused on protecting biodiversity in general and not a particular species. The response of the Florida mouse to land management practices such as mechanical treatment and prescribed fires is poorly documented. This research examined the population responses of Florida mice on three public lands in central Florida, namely, Bullfrog Creek Mitigation Park in Hillsborough County, Split Oak Mitigation Park in Orange County, and Chuluota Wilderness in Seminole County. Florida mice numbers increased or recovered to pre-burn levels within six months following prescribed burns in 2003 and 2004 on the Bullfrog Creek site. Florida mice dropped in numbers following a fire on the Split Oak site, but were increasing when the study ended. The steady decrease in numbers of mice at the Chuluota Wilderness site remained unaffected by habitat modification. Management of public lands that support Florida mice should continue to utilize prescribed fire to maintain upland habitats. When possible, prescribed fires should be limited to the spring and early summer months and applied to only a portion of the total available area in any year.