Variations of the optical detection path-length in image correlation based flow-field measurements result in strong errors in position allocation and thus lead to a strong enhancement of the measurement uncertainty of the velocity. In this contribution we use digital holography to measure the wavefront distortion induced by fluctuating phase boundary, employing spatially extended guide stars. The measured phase information is used to correct the influence of the phase boundary in the detection path employing a spatial light modulator. We analyze the potential of guide stars that are reflected by the phase boundary, i.e. the Fresnel reflex, and transmitted. Our results show, that the usage of wavefront shaping enables to strongly reduce the measurement uncertainty and to strongly improve the quality of image correlation based flow-field measurements. The approaches presented here are not limited to application in flow measurement, but could be useful for a variety of applications.

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第19025号 / 農博第2103号 / 新制||農||1030(附属図書館) / 学位論文||H27||N4907(農学部図書室) / 31976 / 京都大学大学院農学研究科応用生命科学専攻 / (主査)教授 加納 健司, 教授 植田 和光, 教授 植田 充美 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

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Thesis (PhD)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: The aims of this thesis are to identify all possible modes of operaton for a multi-stage axial flow compressor; then to characterise the performance, attempt to numerically model operation, and determine the main flow field features for each mode. Four quadrant axial flow compressor operation occurs when the direction of flow through the compressor or the sign of the pressure difference across the compressor reverses, or any combination of these. Depending on the direction of rotation of the compressor, six modes of operation are possible in the four quadrants of the performance map. The rotor rotates in the design direction for three modes, and in the opposite direction for the other three. The stationary-rotor pressure characteristic is S-shaped and passes through the second and fourth quadrants. A three-stage axial flow compressor operating in the incompressible flow regime was used for the experimental investigation. Flow through the compressor was reversed or augmented by means of an auxiliary axial flow fan. Compressor performance was measured by means of static pressure tappings, a turbine anemometer calibrated to measure forward and reversed volumetric flow and a load cell for torque measurement. The inter-blade row flow fields were measured with pneumatic probes and 50 μm cylindrical hot film probes. Three dimensional single blade-passage Navier-Stokes simulations were performed using the Numeca FineTurbo package. Steady state simulations used a mixing plane approach. A nonlinear harmonic approximation was used for time-unsteady simulations. Unstalled first quadrant operation was unremarkable, and good agreement was obtained between experimental and numerical data. A single stall cell was detected experimentally during stalled operation, which was not modelled numerically. In the fourth quadrant for positive rotation, (windmilling), the compressor acts as an inefficient turbine. Flow separates from the pressure surface of the blade, rendering the steady-state mixing plane approach unsuitable. The performance characteristic curves for second quadrant for positive rotation, are discontinuous with those of first quadrant operation. The temperature rise in the working fluid is significantly higher than at design point. Periodic flow structures occurring across two blade passages were detected at all flow coefficients investigated, invalidating numerical modelling assumptions. Better agreement was obtained between experimental and numerical data from a case found in literature. If the compressor operates as a compressor in reverse (third quadrant operation), significant separation occurs on the pressure surface of all blades, and flow conditions resemble severe first quadrant stall. Separation becomes less severe at larger flow rates, allowing numerical simulation, though this is sensitive to the initial flow field. In the the part of the second quadrant, where the compressor rotates in reverse, it operates as a turbine. The blade angles and the direction of curvature match the flow angles and turning well, leading to high turbine efficiencies. Numerical simulations yielded good agreement with measured results, but were again sensitive to the initial flow field. Fourth quadrant operation with negative rotation occurs when flow is forced through the compressor in the design direction. Large separation bubbles are attached to the pressure surfaces of rotor and stator blades, so virtually all throughflow occurs near the hub and casing / AFRIKAANSE OPSOMMING: Die doelwitte van hierdie tesis is om al die moontlike werkmodusse vir ’n bestaande multi-stadium aksiaalvloei kompressor uit te ken; om dan die gedrag te gekarakteriseer, ’n poging aan te wend om die werking numeries te modelleer, en die belangrikste vloeiveldkenmerke vir elke modus te bepaal. Vier-kwadrant aksiaalvloei kompressor werking vind plaas as die rigting van die vloei deur die kompressor, of die teken van die drukverskil oor die kompressor omkeer, of enige kombinasie daarvan. Afhangende van die rigting van rotasie van die kompressor is ses operasionele modusse moontlik in die vier kwadrante van die kompressorkaart. Die rotor draai in die ontwerprigting vir drie van die modes, en in die teenoorgestelde rigting vir die ander drie. Die stilstaande-rotor drukkarakteristiek is S-vormig gaan deur die tweede en vierde kwadrante. ’n Drie-stadium onsamedrukbare vloei aksiaalvloei kompressor is vir die eksperimentele ondersoek gebruik. Vloei deur die kompressor is omgekeer of aangehelp deur middel van ’n aksiaalvloei hulpwaaier. Kompressor werking is gemeet deur middel van statiese druk meetpunte in die omhulsel, ’n turbine anemometer wat gekalibreer is om vorentoe en omgekeerde volumetriese vloei te meet, en ’n lassel vir wringmoment meting. Interlemryvloeivelde is opgemeet met pneumatiese sensors en 50-μm silindriese warm film sensors. Drie-dimensionele Navier-Stokes simulasies is uitgevoer vir ’n enkele lem van elke lemry, met behulp van die Numeca FineTurbo sagtewarepakket. ’n Mengvlakbenadering is gebruik vir bestendige toestand simulasies, terwyl ’n nie-linere harmoniese benadering gebruik is vir die tyd-afhanklike simulasies. Ongestaakte eerste kwadrant werking was alledaags, en goeie ooreenkoms is gevind tussen die eksperimentele en numeriese data. ’n Enkele staak-sel is eksperimenteel ontdek tydens gestaakte werking. Gestaakte werking is nie numeries gemodelleer nie. In die vierde kwadrant vir positiewe rotasie, (”windmeulwerking”), werk die kompressor as ’n ondoeltreffende turbine. Vloei-wegbrekinging op die lem drukoppervlaktes maak die bestendige toestand mengvlakbenadering ongeskik. In die kenlyne vir tweede kwadrant positiewe rotasie, is daar ’n diskontinu¨ıteit in die prestasie karakteristiekkrommes vir die eerste en tweede kwadrant werking. Die temperatuurstyging in die werk- vloeistof is beduidend ho¨er as as by die ontwerppunt. Periodiese vloeistrukture wat oor twee lemme plaasvind is gevind by alle vloei ko¨effisi¨ente wat ondersoek is, en dit maak die numeriese modellering aannames ongeldig. Beter ooreenkoms tussen die eksperimentele en numeriese data iss verkry met ’n geval wat uit die literatuur gevind is. Indien die kompressor werk as ’n kompressor in omgekeerde (derde kwadrant weking), vind beduidende wegbreking op die drukoppervlak van al die lemme plaas, wat lyk soos ernstige gestaakte eerste kwadrant werking. Die vloeiskeiding raak minder ernstig by ’n groter vloeitempo, wat numeriese nabootsing toelaat, maar die nabootsings is sensitief vir die aanvanklike vloeiveld. In die tweede kwadrant, by omgekeerde rotasie, werk die kompressor as ’n turbine. Die lemhoeke en die rigting van lemkromming stem ooreen met die vloeihoeke en verwringing, wat lei tot ho¨er turbine doeltreffendheid. Numeriese nabootsings stem goed ooreen met gemete resultate, maar is weereens sensitief vir die keuse van die aanvanklike vloeiveld. Vierde kwadrant werking met negatiewe rotasie vind plaas wanneer die lug gedwing word om deur die kompressor in die ontwerprigting te vloei. Groot skeidingborrels sit vas aan die drukoppervlaktes van alle lemme, sodat meeste deurvloei naby die naaf en die omhulsel plaas vind.

: L’une des méthodes qui consiste à optimiser la production d’hydrocarbure est basée sur l’injection d’eau, viscosifiée par addition de polymères de type polyacrylamides, afin d’améliorer l’extraction de pétrole (RAH). Or, les propriétés viscosifiantes des polymères dépendent de leurs masses molaires. L’enjeu de ce travail de thèse a donc été de mettre en place de nouvelles méthodes pour la détermination de larges distributions en masses molaires et de grande dispersité, caractéristiques propres aux échantillons d’intérêt industriel dans le domaine pétrolier. La stratégie d’analyse retenue repose sur la chromatographie d’exclusion stérique (CES) et le fractionnement par couplage flux force (A4F) couplés à la diffusion de lumière et à la réfractométrie. Des polymères modèles ont été synthétisés par un procédé de polymérisation par transfert de chaîne réversible par addition fragmentation (RAFT/MADIX). Cela a permis d’obtenir des polymères dans une gamme de masses molaires comprises entre 103 et 107 g/mol et avec une dispersité inférieure à 1,4. Via l’utilisation de ces polymères, les performances et limites des deux méthodes séparatives investies ont été évaluées. Les conditions opératoires en A4F ont été déterminées et cette méthode s’est avérée être adaptée à l’analyse de polymères industriels distribués sur 3 décades de masses molaires. Au delà de l’analyse dimensionnelle, le couplage avec l’A4F a également permis une analyse conformationnelle. / One method for optimizing the production of hydrocarbon is based on the injection of water, viscosified by the addition of polymers such as polyacrylamides, in order to enhance the oil extraction (EOR). The viscosifying properties of polymers depend on their molecular masses. The aim of this thesis was therefore to develop new methods for determining wide molecular mass distributions and high dispersity, which are specific characteristics of samples of industrial interest in the oil sector. The analytical strategy used is based on size exclusion chromatography (SEC) and flow field flow fractionation (A4F), coupled to light scattering and refractometer. Model polymers were synthesized by a polymerization process by reversible addition fragmentation chain transfer (RAFT/MADIX). This allowed to obtain polymers in a range of molar masses between 103 and 107 g / mol and with a dispersity lower than 1.4. Through the use of these polymers, the capabilities and limitations of the two separation methods invested were evaluated. The A4F operating conditions were determined and this method has proved to be adapted to the analysis of industrial polymers distributed over 3 decades of molecular masses. Beyond the dimensional analysis, A4F-based coupling also allowed conformational analysis.

A body exposed to hypersonic flow is subjected to extremely high wall heating rates, owing to the conversion of the kinetic energy of the oncoming flow into heat through the formation of shock waves and viscous dissipation in the boundary layer and this is one of the main concerns in the design of any hypersonic vehicle. The conventional way of tackling this problem is to use a blunt fore-body, but it also results in an increase in wave drag and puts the penalty of excessive load on the propulsion system. An alternative approach is to alter the flow field using external means without changing the shape of the body; and several such methods are reported in the literature. The superiority of such methods lie in the fact that the effective shape of the body can be altered to meet the requirements of low wave drag, without having to pay the penalty of an increased wall heat transfer rate. Among these techniques, the use of local energy addition in the freestream to alter the flow field is particularly promising due to the flexibility it offers. By the suitable placement of the energy source relative to the body, this method can be effectively used to reduce the wave drag, to generate control forces and to optimise the performance of inlets. Although substantial number of numerical investigations on this topic is reported in the literature, there is no experimental evidence available, especially under hypersonic flow conditions, to support the feasibility of this concept. The purpose of this thesis is to experimentally investigate the effect of energy deposition on the flow-field of a 120� apex angle blunt cone in a hypersonic shock tunnel. Energy deposition is done using an electric arc discharge generated between two electrodes placed in the free stream and various parameters influencing the effectiveness of this technique are studied. The effect of energy deposition on aerodynamic parameters such as the drag force acting on the model and the wall heat flux has been investigated. In addition, the unsteady flow field is visualised using a standard Z-type schlieren flow visualisation setup. The experimental studies have shown a maximum reduction in drag of 50% and a reduction in stagnation point heating rate of 84% with the deposition of 0.3 kW of energy. The investigations also show that the location of energy deposition has a vital role in determining the flow structure; with no noticeable effects being produced in the flow field when the discharge source is located close to the body (0.416 times body diameter). In addition, the type of the test gas used is also found to have a major influence on the effectiveness of energy deposition, suggesting that thermal effects of energy deposition govern the flow field alteration mechanism. The freestream mass flux is also identified as an important parameter. These findings were also confirmed by surface pressure measurements. The experimental evidence also indicates that relaxation of the internal degrees of freedom play a major role in the determination of the flow structure. For the present experimental conditions, it has been observed that the flow field alteration is a result of the interaction of the heated region behind the energy spot with the blunt body shock wave. In addition to the experimental studies, numerical simulations of the flow field with energy deposition are also carried out and the experimentally measured aerodynamic drag with energy deposition is found to match reasonably well with the computed values.

A virus-like particle (VLP) is a biological nanoparticle. It consists of the protective protein shell of a virus that is devoid of the nucleic acid required for viral replication. VLPs have two key uses: they can act as vaccines by inducing an immune response similar to their native virions, or they can facilitate gene therapy and drug delivery by encapsulating non-viral molecules and efficiently transporting them into cells. Manufacture of VLPs involves cell-based expression of virus-shell protein, with particle assembly and purification following one of two paradigms: (i) in vivo VLP assembly, followed by purification of full particles from cell lysate; (ii) partially assembled protein is recovered from cell lysate and assembled into VLPs in vitro. The flexibility and efficiency of both of these VLP manufacturing paradigms can be improved by first gaining a fundamental understanding of what is happening at key process steps. These improvements will lower the cost of VLP manufacture and enhance the viability of VLP products in the biopharmaceutical marketplace. The research reported here yielded positive outcomes for two key steps of the VLP manufacturing process, using murine polyomavirus VLPs for all experimentation. Firstly, enhanced understanding concerning the capture of virus shell protein in pentamer form (capsomeres) from cell lysate using glutathione-S-transferase (GST) affinity chromatography was obtained. It was discovered that prokaryotic expression of GST-tagged capsomeres yielded soluble aggregates having variable size distribution. Methods were developed to physically and chemically characterise these soluble aggregates, and the mechanism by which they adsorb to the chromatography resin was described using an established mathematical model. Secondly, particle characterisation of whole VLPs isolated from cell lysate was undertaken. Methods utilizing three orthogonal and quantitative techniques were developed to suggest that encapsulation of non-viral molecules (nucleic acids or proteins) during in vivo assembly causes distinct changes to the size distribution of isolated VLPs: transmission electron microscopy (TEM), asymmetrical flow field-flow fractionation with multiple-angle light scattering (AFFFF-MALS) and electrospray differential mobility analysis (ES-DMA). The understanding gained from the research presented in this work enables the enhanced capture of partially assembled virus shell protein from cell lysate, as well as a method to efficiently and cost-effectively analyse VLP solutions for the presence of desirable or undesirable encapsulated material.

A virus-like particle (VLP) is a biological nanoparticle. It consists of the protective protein shell of a virus that is devoid of the nucleic acid required for viral replication. VLPs have two key uses: they can act as vaccines by inducing an immune response similar to their native virions, or they can facilitate gene therapy and drug delivery by encapsulating non-viral molecules and efficiently transporting them into cells. Manufacture of VLPs involves cell-based expression of virus-shell protein, with particle assembly and purification following one of two paradigms: (i) in vivo VLP assembly, followed by purification of full particles from cell lysate; (ii) partially assembled protein is recovered from cell lysate and assembled into VLPs in vitro. The flexibility and efficiency of both of these VLP manufacturing paradigms can be improved by first gaining a fundamental understanding of what is happening at key process steps. These improvements will lower the cost of VLP manufacture and enhance the viability of VLP products in the biopharmaceutical marketplace. The research reported here yielded positive outcomes for two key steps of the VLP manufacturing process, using murine polyomavirus VLPs for all experimentation. Firstly, enhanced understanding concerning the capture of virus shell protein in pentamer form (capsomeres) from cell lysate using glutathione-S-transferase (GST) affinity chromatography was obtained. It was discovered that prokaryotic expression of GST-tagged capsomeres yielded soluble aggregates having variable size distribution. Methods were developed to physically and chemically characterise these soluble aggregates, and the mechanism by which they adsorb to the chromatography resin was described using an established mathematical model. Secondly, particle characterisation of whole VLPs isolated from cell lysate was undertaken. Methods utilizing three orthogonal and quantitative techniques were developed to suggest that encapsulation of non-viral molecules (nucleic acids or proteins) during in vivo assembly causes distinct changes to the size distribution of isolated VLPs: transmission electron microscopy (TEM), asymmetrical flow field-flow fractionation with multiple-angle light scattering (AFFFF-MALS) and electrospray differential mobility analysis (ES-DMA). The understanding gained from the research presented in this work enables the enhanced capture of partially assembled virus shell protein from cell lysate, as well as a method to efficiently and cost-effectively analyse VLP solutions for the presence of desirable or undesirable encapsulated material.

Liposomes are versatile biocompatible and biodegradable carriers for a variety of medical applications. As the first nanoparticles, they have been approved for pharmaceutical use so far, and many liposome-based preparations are in clinical trials. Classical methods of liposome preparation represent potential limitations in technology transfer from laboratory to industrial scale. New, microfluidic techniques overcome these limitations and offer new possibilities for controlled, continuous preparation of liposomal particles in a laboratory and industrial scale. An important element in the development of new nanoparticle systems is their complex characterization and purification. In addition to the established chromatographic techniques, the Field flow fractionation technique, in particular the Asymmetrical flow Field-flow fractionation, is described. This relatively new technique in conjunction with the MALS/DLS/DAD-UV/dRI online detectors enables the purification and characterization of complex samples. The main advantage of this technique lies in the possibility of separation under native conditions, which plays an important role in the separation of biopolymers in particular. Separation in the “empty” channel then eliminates sample degradation due to unwanted interactions at the stationary phase-sample interface. The theoretical part of this thesis describes the possibilities of preparation, modification, and characterization of liposomal nanoparticles. For this purpose, optical methods based on dynamic light scattering, multi-angle dynamic light scattering and nanoparticle tracking analysis techniques are described, as well as a non-optical method using "particle by the particle" analysis, tunable resistive pulse sensing method. A separate chapter of the theoretical part is dedicated to the technique Asymmetrical flow Field-flow fractionation in connection with the above-mentioned detectors. Important results associated with this work are summarized in the attached scientific paper, together with the result summaries and the author's contributions.

This master thesis deals with determination of boundaries of the flow from a benchmark automotive vent (right-front situated – in front of a front passenger) using the smoke visualization. In this thesis is tested a pack of eight different constructional variants which differ among them by the type of deflection grille. The angles of the flow borders are compared to specific directional requirements (defined by ŠKODA AUTO a.s.) and there is evaluated a directability of each of the constructional variant on the bases of these results. The results of experiments show that better directability in vertical plane achieve deflection grilles with horizontal vanes situated closer to orifice of the vent or deflection grilles with higher number of horizontal vanes and lower number of vertical vanes (independently of their location). Directability of the deflection grilles in horizontal plane is analogically better with vertical vanes situated closer to orifice of the vent or with deflection grilles with higher number of vertical vanes and lower number of horizontal vanes (independently of their location).