Thesis (M.S.)--Brigham Young University. Dept. of Chemistry and Biochemistry, 2004. / Includes bibliographical references.

Thesis (Ph. D.)--Ohio State University, 2004. / Title from first page of PDF file. Document formatted into pages; contains xviii, 265 p.; also includes graphics (some col.). Includes abstract and vita. Advisor: Walter R. Lempert, Dept. of Mechanical Engineering. Includes bibliographical references (p. 198-210).

Thesis (M. Phil.)--Hong Kong University of Science and Technology, 2003. / Includes bibliographical references (leaves 78-84). Also available in electronic version. Access restricted to campus users.

Due to the biological importance of heme and its implication in various disease states, uncovering how it is transported throughout the cell is of vital importance. Some of the strongest in vivo tools present in the literature are FRET-based sensors using a number of chromophores that are optimized and expanded from GFP. In order to elucidate the movement of heme throughout the cell, GFP FRET -based heme sensors were designed, expressed, and purified to be further characterized in vitro. This series of heme sensors were expressed in Saccharomyces cerevisiae to monitor the in vivo movement of heme. Different growth conditions were explored to monitor the effect of these changes to cytosolic heme availability. These heme sensors are now poised to address the movement of heme from the mitochondria to other targets in the cell under a variety of conditions. This will provide insight into heme trafficking pathways, as well as the role heme plays in neurodegenerative diseases and aging

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Herein is presented the collective experimental record of research performed in the Laboratory for Biological Surface Science. These investigations are generally classified under the category of bioanalytical surface science and include the following projects. Chapters III and IV describe the creation of a microfluidic device capable of generating fixed arrays of concentration gradients. Experimental results were matched with computational fluid dynamics simulations to predict analyte distributions in these systems. Chapters V and VI demonstrate the discovery and utility of photobleaching fluorophores for micropatterning applications. Bleached fluorophores were found to rapidly attach to electron rich surfaces and this property was used to pattern enzymes inside microfluidic channels in situ. Finally, Chapter VII exhibits a method by which solid supported lipid bilayers can be dried and preserved by specifically bound proteins. The intrinsic property of lateral lipid mobility was maintained during this process and a mechanism by which the protein protects the bilayer was suggested.