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Fluorescein and Rosamine Derivatives as Donors/Acceptors for "Through-bond" Energy Transfer CassettesCastro, Juan C. 2009 December 1900 (has links)
A series of fluorescein and rosamine derivatives have been prepared and their
spectroscopical properties analyzed to determine their usefulness as donor and/or
acceptors in "through-bond" energy transfer systems.
Such new systems have been tailored to possess higher quantum yields, increased
water solubility and higher pH independence. Some of these compounds have also been
designed with handles for bioconjugation for use in intracellular imaging.
The syntheses of most of these compounds have been optimized to afford higher
yields in a multigram scale.
Single molecule studies on a fluorescein/rosamine cassette are also reported.
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