No description available.

No description available.

No description available.

No description available.

This study adapted and developed methods of assessing and modelling biodiversity of butterflies and day-flying moths in habitat fragments, and determined those factors affecting their presence, abundance and species richness in a sample of 46 isolated urban remnants in south-west Western Australia. The specific objectives were to: (i) assess the effectiveness of transect–based sampling to quantify the species richness of habitat fragments; (ii) examine patterns of species richness in habitat fragments and quantify the detectability of each species recorded; (iii) review and rationalize the methods used to fit species–area–habitat models; and (iv) model species incidence, abundance and total richness of butterflies in urban habitat fragments and determine implications and priorities for their conservation. / These objectives were achieved and the principal findings of the research are: (i) The transect method provides an accurate assessment of butterfly species richness in isolates provided that the level of sampling (proportion of area surveyed) is adequate, that sufficient surveys are conducted during the flight season to ensure high levels of detectability, and that surveys are conducted at appropriate times and during suitable weather conditions. Although randomly placed transects are preferable, logistic constraints often dictate the use of existing pathways, roadsides or management tracks – which requires the use of longer transects but is more practical in urban remnants. / (i) The transect method provides an accurate assessment of butterfly species richness in isolates provided that the level of sampling (proportion of area surveyed) is adequate, that sufficient surveys are conducted during the flight season to ensure high levels of detectability, and that surveys are conducted at appropriate times and during suitable weather conditions. Although randomly placed transects are preferable, logistic constraints often dictate the use of existing pathways, roadsides or management tracks – which requires the use of longer transects but is more practical in urban remnants. / (iii) Almost a century of fitting species–area curves has failed to produce agreement on which function is the best model of the relationship. Many of the proposed functions are identical, special cases of others or have arisen from transcription errors. Empirical comparison of these functions requires methods suited to the distribution of species number such as the generalized linear model, method of maximum likelihood and the information-theoretic approach, and proper attention to covariates and their interactions. / (iv) Site area and vegetation condition were the dominant determinants of the presence, abundance and total species richness of resident butterflies and day-active moths in 46 urban habitat fragments in south-west Western Australia. Larger sites with more high quality (undisturbed) vegetation favoured 16 of 20 native species and only one benefited from disturbance. A further nine species not sufficiently widespread or abundant to enable individual analysis were collectively more prevalent in larger sites. Resource quality and quantity dominated the patterns of site occupancy, and increased site connectivity did not favour any species – results consistent with habitat resources, not metapopulation effects, determining current distribution patterns. As expected, the presence of non-resident species was unaffected by site area. The total number of resident species at each site reflected the collective responses of the individual species: increasing with area and declining with vegetation disturbance. The effects of area and vegetation quality were not simply additive: disturbance had a far greater impact on small remnants. This interaction is inconsistent with the area per se hypothesis: in the absence of disturbance there was no evidence of a species–area effect. / This study is the first comprehensive, quantitative assessment of the distribution and ecology of butterflies and day-flying moths in Australian urban habitat fragments and provides a baseline against which future changes in species distributions may be measured. The results have important implications for the conservation of butterflies and day-flying moths in the region. Maintenance of vegetation quality is of paramount importance and is vital in smaller remnants. Large remnants, being less susceptible to local extinctions, will be essential for the persistence of many species. Many functions have been proposed to model the species–area relationship but empirical comparisons have been hindered by methodological problems – this study conducted a re-examination of the relationship and presents an appropriate framework to compare functions. This study is also one of few to demonstrate and quantify the importance of interactions in explaining patterns of species richness and should stimulate future research into the importance of these effects.

No description available.

In Chapter 1, the literature relating to rabies virus and the rabies like lyssaviruses is reviewed. In Chapter 2 data are presented from 1170 diagnostic submissions for ABLV testing by fluorescent antibody test (Centocor FAT). All 27 non-bat submissions were ABLV-negative. Of 1143 bat accessions 74 (16%) were ABLV-positive, including 69 of 974 (7.1%) flying foxes (Pteropus spp.), 5 of 7 (71.4%) Saccolaimus flaviventris (Yellow-bellied sheathtail bats), none of 151 other microchiropteran bats, and none of 11 unidentified bats. Statistical analysis of data from 868 wild Black, Grey-headed, Little Red and Spectacled flying foxes (Pteropus alecto, P. poliocephalus, P. scapulatus, and P. conspicillatus) indicated that three factors; species, health status and age were associated with significant (p&lt 0.001) differences in the proportion of ABLV-positive bats. Other factors including sex, whether the bat bit a person or animal, region, year, and season submitted, were not associated with ABLV. Case data for 74 ABLV-positive bats, including the circumstances in which they were found and clinical signs, is presented. In Chapter 3, the aetiological diagnosis was investigated for 100 consecutive flying fox submissions with neurological signs. ABLV (32%), spinal and head injuries (29%), and neuro-angiostrongylosis (18%) accounted for most neurological syndromes in flying foxes. No evidence of lead poisoning was found in unwell (n=16) or healthy flying foxes (n=50). No diagnosis was reached for 16 cases, all of which were negative for ABLV by TaqMan® PCR. The molecular diversity of ABLV was examined in Chapter 4 by sequencing 36 bases of the leader sequence, the entire N gene, and start of the P gene of 28 isolates from pteropid bats and 3 isolates from Yellow-bellied sheathtail (YBST) bats. Phylogenetic analysis indicated all ABLV isolates clustered together as a discrete group within the Lyssavirus genera closely related to rabies virus and European bat lyssavirus-2 isolates. The ABLV lineage consisted of two variants; one (ybst-ABLV) consisted of isolates only from YBST bats, the other (pteropid-ABLV) was common to Black, Grey-headed and Little Red flying foxes. No associations were found between the sequences and either the geographical location or year found, or individual flying fox species. In Chapter 5, 15 inocula prepared from the brains or salivary glands of naturally-infected bats were evaluated by intracerebral (IC) and footpad (FP) inoculation of Quackenbush mice in order to select and characterize a highly virulent inoculum for further use in bats (Inoculum 5). In Chapter 6, nine Grey-headed flying foxes were inoculated with 105.2 to 105.5 MICED50 of Inoculum 5 divided into four sites, left footpad, pectoral muscle, temporal muscle and muzzle. Another bat was inoculated with half this dose divided into the footpad and pectoral muscle only. Seven of 10 bats developed clinical disease of 1 to 4 days duration between PI-days 10 and 19 and were shown to be ABL-positive by FAT, HAM immunoperoxidase staining, virus isolation in v mice, and TaqMan PCR. Five of the seven bats displayed overt aggression, one died during a seizure, and one showed intractable agitation, pacing, tremors, and ataxia. Viral antigen was demonstrated throughout the central and peripheral nervous systems and in the epithelial cells of the submandibular salivary glands (n=4). All affected bats had mild to moderate non-suppurative meningoencephalitis and severe ganglioneuritis. No ABLV was detected in three bats that remained well until the end of the experiment on day 82. One survivor developed a strong but transient antibody response. In Chapter 7, the relative virulence of inocula prepared from the brains and salivary glands of experimentally infected flying foxes was evaluated in mice by IC and FP inoculation and TaqMan assay. The effects in mice were correlated to the TaqMan CT value and indicated a crude association between virulence and CT value that has potential application in the selection of inocula. In Chapter 8, 36 Black and Grey-headed flying foxes were vaccinated with one (day 0) or two (+ day 28) doses of Nobivac rabies vaccine and co-vaccinated with keyhole limpet haemocyanin (KLH). All bats responded to the Nobivac vaccine with a rabies-RFFIT titer &gt 0.5 IU/mL that is nominally indicative of protective immunity. Plasma from bats with rabies titres &gt 2 IU/mL had cross-neutralising ABLV titres &gt 1:154. A specifically developed ELISA detected a strong but transient response to KLH.

Thesis (M.S.)--Rochester Institute of Technology, 2004. / Typescript. Includes bibliographical references (leaves 116-117).

Thesis (Ph. D.)--University of Hawaii at Manoa, 2004. / Includes bibliographical references (leaves 134-149).

Thesis (Ph. D.)--Louisiana State University and Agricultural and Mechanical College, 2004. / Includes bibliographical references. Also available online (PDF file) by a subscription to the set or by purchasing the individual file.