Thesis (Ph. D.)--University of Washington, 2007. / Vita. Includes bibliographical references (p. 217-248).

Thesis (Ph. D.)--Oregon State University, 2008. / Printout. Includes bibliographical references (leaves 85-94). Also available on the World Wide Web.

Same of the philosophical aspects of modelling are discussed along with the importance of understanding primary marine food-web components. Howe Sound, a coastal embayment on the British Columbia coast, is examined as a base study area, and field sampling methods as well as laboratory techniques are summarized. The main body of the investigation involves the development of a mathematical description of phytoplankton population growth and distribution as a function of biological and physical circulation parameters in the sound. This is accomplished by dividing the sound into zones and modelling primary productivity as a result of certain key environmental forcing functions. Transport between zones is shown to affect spatial timing and distribution. Observed and predicted values of nutrients, temperature, extinction coefficients, zooplankton biomass, and phytoplankton productivity and biomass are compared as the model is refined. It is then used to simulate the effects of a delayed spring on productivity in Howe Sound, as well as to model growth in Indian /Arm, an adjacent embayment. Simulated annual productivity in Have Sound is 235, 316 and 384 gC‧m⁻²‧yr⁻¹ in the down inlet direction for the three model zones. With a delayed spring the values are reduced to 200, 308 and gC‧m⁻²‧yr⁻¹ and comparisons are made with observed data in Howe Sound in 1974 when poor spring weather conditions prevailed. In Indian Arm the model predicts a spatial productivity distribution of 318, 256 and 239 gC‧m⁻²‧yr⁻¹, values which agree with field observations of two workers. The general applicability of such models to complex ecosystems is discussed. / Science, Faculty of / Zoology, Department of / Graduate

Benthic algal ecology and primary pathways of energy flow were considered on the Squamish River delta at the head of Howe Sound, a fjord-type estuary. The study elucidated the structure and function of major autotrophic components of the estuarine ecosystem. Benthic algae were investigated with regard to species composition and distribution and the capacity for energy conversion, input to the system and storage. Comparisons were made with existing information on the vascular plant component of the ecosystem. The benthic algal community was studied by regular field sampling of major macroalgae and microalgal associations with a monitoring of physical-chemical environmental factors. Presence of an alga in the estuary was a function of its osmoregulatory capabilities. Establishment and temporal-spatial distribution patterns were controlled by substrate-habitat preference and availability and the interaction of light, interspecies competition, desiccation, temperature and salinity, light being of greatest importance. Carex lyngbyei Hornem., the dominant vascular plant, had a significant effect on distribution of benthic algae through light restriction during Its summer growth period and action as a substrate during the winter. Total species diversity, biomass and distributional area of benthic algae were greatest at the latter period. The effect of ecosystem structure on function was investigated by analysis of energy flux through major benthic algal producers. Comparisons were made of the total amount of energy input attributable to benthic algae and vascular plants. The importance of an algal producer to energy flux•m⁻² was a function of either high primary productivity, photosynthetic efficiency and caloric content, or in the case of diatom dominated micro-algal associations, high caloric content alone. Distribution, reflecting the presence of suitable substrate-habitat, modified this pattern. Macroalgae having high energy input•m⁻² (Monoetroma oxyapermum (Kutz.) Doty, Pylaiella littoral-is (Lyngb.) Kjell.) were of minimum importance to total energy input. Two microalgal associations (Association E, diatom dominated, Association G, Ulothrix flaoca (Dill.) Thur. dominated), each with low energy input-m but with wide distribution and high photosynthetic efficiency and caloric content contributed a total of 8H% of available energy attributable to benthic algae. Benthic algae account for a maximum of ca. 7% of total energy input to the delta ecosystem compared to ca. 90$ by vascular plants and 3% by addition of organic matter. The majority of energy for the detrital based ecosystem comes from vascular plants and becomes available after a lag period allowing decomposition. Benthic algae are significant to the ecosystem as a readily available, continually present energy source requiring little or no breakdown for utilization and not for total energy input. Energy is available as either dissolved or particulate organic matter. Of the latter, ca. 49$ is removed to the estuary, 33% incorporated into the sediments of the delta and 18$ used by consumers in the delta ecosystem. / Science, Faculty of / Botany, Department of / Graduate

The increasing pollution of the aquatic environment by cadmium is a potentially severe problem and techniques are needed to document the effect of the metal. To investigate the flux of this metal through a laboratory food chain, algae were grown in various cadmium concentrations for subsequent use as contaminated food for mussels. The results showed that in order to make valid deductions, more information about chemical mechanisms and background ecophysiological data is needed, otherwise accumulation reports may become misleading. It was found that the best growth and accumulation results were achieved by harvesting algae from a zinc deficient media containing 7 μmole dm-3 cadmium and at a particular life cycle phase. Two uptake mechanisms are proposed. These "contaminated" algae were fed to mussels under different accumulation regimes. The metal gain and loss were determined and compared to a "baseline" dry body weight which had been calculated from a shell length-body weight relationship. Cadmium accumulation took place in the mussels and after some initial delay, could be correlated to weight loss. Such a weight loss was due to pathological and biochemical changes in the mussels. It was shown that the toxic effect of cadmium could be determined much earlier by the presence of special proteins. The elutant profiles of the gel chromatography study showed the production of metal binding protein as well as a spill over of cadmium into the enzyme pool, caused by a higher uptake than elimination rate. Cadmium on metal binding protein and in the enzyme pool could be related to the poisoning effect of the metal and a pollution history for the mussels identified. The characteristics of the metal binding protein were found to be very similar to those reported for metallothionein and had an approximate molecular weight of 10 600 daltons.

No description available.

In situ microcosms of a planktonic community were exposed to the triazine herbicide simazine. Randomly selected sets of microcosms were collected and sampled each week for three weeks (plus Week 0). Samples of ambient water were collected each week for evaluation of enclosure effects. Physical and chemical parameters were measured per microcosm. Simazine was measured at Weeks 0 and 3 only. The following organisms were preserved and quantified: phytoplankton, bacteria, and zooplankton, including ciliates, copepod nauplii, cladocerans and rotifers. Simazine decreased dissolved oxygen and pH, but increased nitrate and ammonia concentrations compared to control microcosms. A temporary decrease in temperature occurred at Week 1. Phytoplankton were differentially affected by simazine. Sensitive taxa included Trachelomonas, Glenodinium, diatoms and several species of relatively minor significance. Dinobryon and miscellaneous coccoids were not significantly affected. Phytoplankton ≥9 um were more affected by simazine than phytoplankton <9 um. Many cells <9 um may be facultative or obligate heterotrophs and not susceptible to simazine. Although data were variable, bacteria were also not affected by phytoplankton changes or simazine. Rotifers were the major zooplankters and the two dominant species, Kellicottia bostoniensis and Keratella cochlearis, were reported to graze exclusively on cells <9 um. Polyarthra vulgaris and Synchaeta pectinata also graze in this size range but are not limited to it. Copepod nauplii/copepodids were present, but adult copepods and cladocerans were rare. The tintinnid ciliate Codonella exhibited a temporary population increase during the study. Zooplankton were not affected by simazine-induced changes in the phytoplankton. Kellicottia bostoniensis was the only zooplankter affected by simazine: it had lesser mortality in higher concentrations of simazine. Possible reasons for this enhanced survival were discussed. The zooplankton (primarily rotifers) appeared to feed more on heterotrophic cells than on autotrophic cells, largely as a function of food size, and may have been more closely associated with the detrital food chain than the autotrophic food chain. / M.S.

Thesis (M.S.)--Oregon State University, 2004. / Typescript (photocopy). Includes bibliographical references. Also available via the World Wide Web.

published_or_final_version / Education / Doctoral / Doctor of Philosophy

published_or_final_version / Biological Sciences / Master / Master of Philosophy