Stable isotope analysis is a tool employed in ecological studies to provide information on the movement of elements and energy through a system. The stable carbon and nitrogen isotope analysis of aquatic organisms has been commonly used to address questions related to energy transfer between organisms and to identify the reliance of aquatic organisms on different sources of organic matter within the system. Within the rivers, stable isotope analysis has been used to describe food webs and connect conditions within the watershed with the river. The Grand River watershed is a predominantly agricultural watershed which receives inputs from ~26 MWWTP and is managed for flow by multiple large reservoirs and weirs. The stable isotope values of aquatic organisms within this watershed were analyzed from samples collected between May and September, 2007. Sites were selected in relation to three different municipal waste water treatment plants (MWWTP) in the centre of the watershed and along a 200 km stretch of the main stem of the Grand River. Results show that stable isotope analysis can be used to differentiate organisms collected from different sites and which represent different trophic levels within the river system under select conditions. Sites which are influences by inputs from organic matter or nutrients within distinct isotope values can be distinguished easily if the input is large and the isotope values are significantly distinct from background values. For smaller inputs changes in stable isotope values were not observed relative the background variability in the system. In this case, sites should be selected to allow for the characterization the variation in isotope values already occurring within the river. Samples collected later in the growing seasons have more distinctive isotope values are between sites. At sites where seasonal variation is greater, the organisms collected may not show a clear separation between trophic levels. A lack of knowledge regarding the time period represented by the tissues of the organisms challenges interpretation these results. It is concluded that stable isotope values of aquatic organisms reflected the condition of this watershed. For nitrogen increasing loads from point sources were accompanied by increasing isotope values. Stable isotope values decreased over the river reach where recovery in river condition occurs as a result of ground water inputs. The influence of individual large MWWTP and reservoirs was observable and the management of the MWWTP and reservoir appears to affect the changes in isotope values which are observed.

Much of the world's species diversity is located in tropical and sub-tropical ecosystems, and a better understanding of the ecology of these systems is necessary to stem biodiversity loss and assess community- and ecosystem-level responses to anthropogenic impacts. In this dissertation, I endeavored to broaden our understanding of complex ecosystems through research conducted on the Cinaruco River, a floodplain river in Venezuela, with specific emphasis on how a human-induced perturbation, commercial netting activity, may affect food web structure and function. I employed two approaches in this work: (1) comparative analyses based on descriptive food web characteristics, and (2) experimental manipulations within important food web modules. Methodologies included monthly sampling of fish assemblages using a variety of techniques, large-scale field experiments, extensive stomach content and stable isotope analyses. Two themes unite the information presented: (1) substantial spatial and temporal variability in food web structure, and (2) how body-size can be used to generalize species-interactions across this complexity. Spatial variability occurred at various scales, from among small fish assemblages on seemingly homogeneous sand banks, to differences among landscape scale units (e.g. between lagoons and main river channel). Seasonal variability was apparent in predation patterns, with relative prey availability and body size primarily resulting in decreasing prey sizes with falling water levels. Body size was also related to functional outcomes of species interactions, for example, a size-based response of prey fishes to large-bodied piscivore exclusion. This pattern was further substantiated at the landscape-scale, as differences in assemblage structure among netted and un-netted lagoons were largely size-based. Trophic position of fish and body size was not found to be related, likely due to the diversity of prey available to consumers, and may signify that commercial netting activity will not decrease food chain lengths. In sum, by describing human impacts within a food web context, I endeavor to provide predictive power regarding a specific human-induced environmental problem, yet still allowing for generality that will broaden the theoretical foundations and applications of food web ecology.

Food webs are good ecological macro-descriptors and their study is important in ecology in understanding nutrient cycles, tracing and quantifying energy and in describing trophic interactions within an ecosystem. The knowledge of food web finds applications in various natural sciences disciplines but also in many productive sectors. This study investigated the structure of the food web of a shallow lake in the Pantanal flood plain. The food web included two macrophytes, six aquatic insects, four crustaceans and 24 fish species. Sources of carbon for the various organisms living in the lake were identified through the values of δ13C exhibited by the organisms. The δ15N signature was used to estimate the trophic position of each organism. A cluster analysis based on the two isotopic signatures revealed six different feeding guilds and emphasized on the broad occurrence of omnivory among animals living in the lake. This study revealed that the use of food carbon was the most important factor that structured the lake community. Very low values of δ13C in zooplankton, benthic dwellers and bottom-feeder organisms as well as similarities between the gradient of δ13C and that of use of methane oxidizing bacteria informed on the possible use of biogenic methane as a source carbon and energy for the lake biota.

Global warming will affect every level of biological organization from the metabolism of individuals to the functioning of ecosystems. I explored the effects of warming on three rock-pool meioinvertebrate communities along a latitudinal gradient (temperate, tropical, and sub-Arctic regions) to determine effects on community and food-web structure and functioning. Warming affected regions differently, having a positive effect on sub-Arctic communities, a negative effect on temperate communities, and intermediate responses in tropical communities in terms of abundance, stability and extinction frequency. Differences in structural properties of the food webs supported the insurance hypothesis: that greater redundancy in webs results in greater stability, and helped to explain why the tropical community was more stable than the temperate community in warmed treatments. My study highlights the importance of considering differential response of species and communities from different latitudes and the importance of food web structure in predicting species response to global climate change.

Fatty acid (FA) analysis is a powerful ecological tool for examining trophic relationships among marine organisms. Its application in benthic food webs may be limited because many benthic organisms consume a highly mixed diet, and FA metabolism of benthic invertebrates may obscure dietary markers. This thesis examines the use of FA as dietary tracers for studying the diets of benthic invertebrates and the fate of primary production in rocky subtidal food webs. In Chapter 2, I review the use of FA for studying benthic marine food webs, and suggest that field studies using FA analysis should also include data from controlled feeding experiments, gut contents, or stable isotope analysis to provide more reliable results. In Chapter 3, I compare FA composition among sea urchins fed four natural algal diets in a controlled feeding study. These sea urchins substantially modified their dietary FA but differed in their overall FA composition according to diet. In Chapter 4, I use FA to trace the invasive alga Codium fragile ssp. fragile and the native kelp Saccharina longicruris through two trophic transfers in an experimental food web. Substantial signal attenuation occurred with each trophic transfer, suggesting that FA analysis may be of limited use for tracing benthic primary producers in field studies. In Chapter 5, I use FA in conjunction with stable isotope analysis and gut contents analysis to investigate the contribution of detrital kelp to the diet of sea urchins in habitats adjacent to kelp beds. FA analysis was unable to distinguish among sea urchins at different distances from the kelp bed, but the results of all analyses indicated that the availability of kelp detritus declines with distance from the kelp bed, and that sea urchins in low-productivity habitats rely on both kelp detritus and benthic diatoms. In Chapter 6, I summarize the overall findings and suggest experimental and statistical methods to address some of the problems associated with using FA analysis to study trophic relationships in benthic food webs.

Biodiversity can be described as the total variation of life forms, where diversity ranges from gene level up to the ecosystem level. The diversity can be calculated in a number of ways, and this study use one of these methods. In this study empirical food webs have been used and analyzed, where eleven characters are defined and used as parameters to calculate the Euclidean distances between food webs that describe the variation that may exist within classes of terrestrial, marine and freshwater habitats. The class who stood out and showed the greatest diversity at the ecosystem level was the marine food webs, which showed a high value of the average euclidean distance. The other networks were not as distinctive and the average of the euclidean distance in these classes was comparatively low.

Stream food webs are supported by carbon produced within the stream (autochthonous) and from terrestrial environments (allochthonous). Allochthonous carbon (C) inputs are assumed to be the dominant C source supporting food webs within small streams, but few direct estimates of resource use in small streams have been made, especially in boreal streams. The objective of this study was to determine the relative dependence on allochthonous and autochthonous C by consumers in relation to C pools within streams with high terrestrial inputs. Furthermore, this study aimed to investigate if the relative resource use of allochthonous and autochthonous C by consumers differed among seasons (summer and fall), between streams of different sizes, and locations within the catchment. To estimate consumer resource use, δ2H signatures for organic C sources were compared to those of six key consumers in five streams of varying catchment sizes in northern Sweden. Macroinvertebrate biomass was quantified to calculate a taxa-specific biomass-weighted allochthony, and compared with the mass of different C pools potentially available for consumers. The biomass-weighted mean allochthony for all samplings ranged between 43.5-61.5%; there was thus high autochthonous support despite low algal density and high terrestrial C pools within the streams. No significant trend in allochthony was observed over season (linear regression, p-value >0.05). Allochthony differed by invertebrate taxa and was not related to stream size or location in catchment. These results suggest that autochthonous C is far more important for consumers in boreal streams than previously recognized.

Generalist predators can contribute to vital ecosystem services by potentially inducing trophic cascades as natural enemies of pests in agroecosystems. As the human population of the world gets larger, we need to produce more food on ever-smaller swaths of available land relying on ecosystem services, in the form of pest control, that may contribute to agricultural sustainability. Teasing apart the exact trophic linkages between predators and prey is a vital first step and essential to uncovering which predators are inducing trophic cascades and should be enhanced through conservation biological control. Combined with ecological experimentation, the main tool used throughout my research to identify trophic linkages is molecular gut-content analysis. I began by investigating mass sampling techniques and found they do not cause contamination in gut-content analysis and may be a simple method for collecting large numbers of cryptic predators for use in determining trophic linkages. Additionally, my research uncovered trophic interactions between stink bugs and generalist predators at multiple scales. Overall, I successfully designed molecular methods to investigate relationships between agricultural pests and generalist predators. A multi-year field study uncovered low predation on stink bug pests in contrast to previous research suggesting that generalist predators were contributing highly to biological control. This research highlights the need for replicated studies before making broad conservation biological control decisions. Although generalist predators were not consuming stink bugs in large numbers, my field cage study showed evidence of superfluous killing by spiders on adult stink bugs, highlighting the need to combine ecological studies with molecular methods to understand consumptive and non-consumptive effects on prey items. Gut-content analysis showed no evidence of consumption, but the field cage study allowed me to uncover the complicated relationships between spiders and stink bugs. In addition, I showed an invasive species can be detected in new areas through molecular gut-content analysis of predators before other sampling methods.

Biodiversity loss is one of the major threats to humanity. This has led to an increasing amount of research on biodiversity on genetic and species levels. Studies of diversity at the ecosystem level has however been neglected. An important aspect of ecosystems is food webs that describe the predation-prey interactions between species. Properties explaining the topological structure of food webs can be used to compare and highlight differences between ecosystems. In the present study, topological network properties are used to compare the diversity of network structures between groups of empirical food webs. Differences between 45 aquatic and 45 terrestrial food webs are compared as well as the effects of species richness on lake network structure diversity. Network structure diversity is measured as the average Euclidean distance from food webs to their group centroid in a multidimensional space of network properties. While the average network structure differs between aquatic and terrestrial food webs, no significant difference in variation is found. For 128 Swedish and 48 North American lake food webs, increasing species richness is shown to decrease network structure diversity. A higher diversity of network structures could potentially indicate a more ways to cope with disturbances or provisions of a higher variety of ecosystem services. Preliminary tests of ecosystem diversity effects on stability were conducted but proved inconclusive.

Given the rapidly increasing visitation levels to Fraser Island, there is increasing concern that tourist activities may threaten the long-term ecological health of the region's unique dune lakes. This project aimed to investigate the consequences of tourist use of Fraser Island's dune lakes and to develop appropriate monitoring tools and management objectives in light of the projected increases in visitation levels in the foreseeable future. The initial phase of this research aimed to identify the relative importance of some of the most popular dune lakes on the island as key destinations for tourists. Tourist surveys, in conjunction with the development of a Tourist Pressure Index (TPI), which quantifies logistic, social and natural variables, identified Lakes McKenzie, Allom and Birrabeen as the lakes most at risk from excessive tourist use. In addition, analyses of water quality in 15 lakes on Fraser Island aimed to determine the current trophic status of dune lakes on Fraser Island and the ecological implications of tourist use of these systems. Detailed comparisons of nutrient and chlorophyll a concentrations in five popular dune lakes in February 1990 (data from Arthington et al. 1990) and February 1999 suggested that productivity has increased significantly in the past decade. More detailed examinations of nutrient and algal variables in five popular perched dune lakes revealed that while ambient nutrient and phytoplankton chlorophyll a concentrations remained relatively stable, periphyton chlorophyll a concentrations increased over the course of the 1999-2000 summer in most lakes. Significantly, these increases were found only in heavily visited (disturbed) sites in the clear lakes examined (McKenzie and Birrabeen). In these lakes, where algal growth is likely to be only limited by nutrient availability, tourist nutrient additions may stimulate excessive periphyton production. Experimental algal bioassays identified that phytoplankton and to a lesser degree periphyton growth was stimulated by nutrient additions in all five perched dune lakes. However, the degree to which growth was stimulated was both lake and nutrient (nitrogen versus phosphorus versus nitrogen + phosphorus) dependent, highlighting the variable nature of systems within a relatively small geographic range. Since periphyton biomass was higher in heavily visited areas of lakes and was likely to be stimulated by nutrient additions by tourists, stable isotope analyses of littoral zone food webs were conducted to quantify the percent contribution of periphyton to consumer diets. There was a trend towards higher periphyton contributions in systems identified as key tourist locations (on the basis of their TPI scores) and this indicates that increasing visitation may increase the contribution of periphyton to littoral zone food webs, both via increases in the quantity and quality of periphyton as a food resource. To further explore the contribution of periphyton in littoral zone food webs of heavily visited lakes, a 15N-tracer addition experiment was conducted to establish the fate of nutrient additions within the littoral zone. Nutrients were added in quantities that mimicked those likely from tourists, to enable a realistic appraisal of the fate of tourist additions. As expected, periphyton rapidly assimilated the added 15N-tracer and was found to be the first and most significant sink for nutrients entering the littoral zone. Finally, the results from this research were used to develop a conceptual model of nutrient enrichment for perched dune lakes on Fraser Island. The model indicates that although nutrient additions from tourists may lead to undesirable increases in periphyton biomass, the degree to which this is deemed to be a detrimental ecological outcome is likely to be mediated by water level fluctuations and the consumptive capacity of grazers. Given that excessive periphyton growth is likely to be seen as negative impact of tourism, regular periphyton monitoring (biomass and percent contribution to littoral zone food webs) should be built into an updated monitoring program for this series of dune lakes. Whilst the implementation of periphyton monitoring is likely to enable the early detection of deleterious impacts of excessive tourist use, it is likely that the long-term conservation of the region will, in the future, require the implementation of strict visitation level guidelines, to ensure that the irreversible consequences of long term additions of nutrients are ameliorated.