In the control of the vibration structure, active vibration control is an important topic during these years. In this thesis, an active vibration control strategy is proposed to improve the vibration problem in a linear motor positioning system. The main purpose of the present research is to reduce the vibration of the positioning system. A force estimator is applied to suppress extra force produced from a linear motor positioning system. However, uncertain parameters of the system and external disturbance degrade the accuracy of the force estimator. A sliding controller has been designed to enhance the performance of the control system. The performance of the force estimator and the improvement of the control strategy have been discussed thoroughly in this research. The computer simulation and experiments both show encouraging results of the proposed control strategy. The vibration induced from a linear motor positioning system can be suppressed to the expected amplitude 0.0956 when the linear motor position completed.