Several forensic sciences, especially of the pattern-matching kind, are increasingly seen to lack the scientific foundation needed to justify continuing admission as trial evidence. Indeed, several have been abolished in the recent past. A likely next candidate for elimination is bitemark identification. A number of DNA exonerations have occurred in recent years for individuals convicted based on erroneous bitemark identifications. Intense scientific and legal scrutiny has resulted. An important National Academies review found little scientific support for the field. The Texas Forensic Science Commission recently recommended a moratorium on the admission of bitemark expert testimony. The California Supreme Court has a case before it that could start a national dismantling of forensic odontology. This article describes the (legal) basis for the rise of bitemark identification and the (scientific) basis for its impending fall. The article explains the general logic of forensic identification, the claims of bitemark identification, and reviews relevant empirical research on bitemark identification-highlighting both the lack of research and the lack of support provided by what research does exist. The rise and possible fall of bitemark identification evidence has broader implications-highlighting the weak scientific culture of forensic science and the law's difficulty in evaluating and responding to unreliable and unscientific evidence.

Studies have highlighted a growing national problem regarding the number of untested Sexual Assault Kits (SAKs). A 2011 National Institute of Justice report revealed Los Angeles alone had 10,000 untested SAKs. This backlog has fueled the need for specific and efficient testing of SAK evidence. In traditional workflows, serology tests are used to indicate the presence of a targeted bodily fluid and prioritize samples for genetic analysis. However, given the lack of sensitivity and specificity of modern serological assays, current SAK workflows often skip serological identification altogether for a “direct to DNA” approach. While these Y-Screen workflows achieve rapid screening of samples for the presence of a detectible male contributor, they do not provide any serological information. As a result, samples lack what can be critical investigative context. Improved serological capabilities with enhanced sensitivity and specificity would provide greater confidence in results for the confirmatory identification of seminal fluid. At a minimum, forensic biologists should understand the limitations associated with traditional serological approaches to seminal fluid identification when processing SAK samples. Current serological techniques based on antigen-antibody binding have exhibited both sensitivity and specificity limitations. False positive results for semen can be obtained by non-target biological fluids such as breast milk, urine, and vaginal fluid, or by non-specific binding events. This study evaluates a promising emerging technique that combines high specificity protein biomarker detection with targeted mass spectrometry. This research targeted human-specific peptide markers for seminal fluid proteins and peptide standards to perform quantification of seminal fluid peptide targets using an Agilent 6495 mass spectrometer coupled to a 1290 series liquid chromatograph. This approach has shown to be both more specific and sensitive in identifying a bodily fluid compared to current immunological based approaches. Thus, this proteomic workflow was used to evaluate authentic false positive rates of current immunochromatographic techniques for seminal fluid identification. Self-collected vaginal swabs collected from participants not engaging in barrier-free vaginal intercourse with male partners were tested using various immunochromatographic assays designed to detect both semenogelin (Sg) (RSID™-Semen) and prostate specific antigen (PSA) (ABAcard® p30 Test and SERATEC® PSA Semiquant). Similarly, three seminal fluid biomarkers (semenogelin 1, semenogelin 2, and prostate specific antigen) were used for seminal fluid identification via mass spectrometry. Any samples producing positive results on any immunochromatographic assay were evaluated to determine whether the target protein was actually present at levels above the reported sensitivity limits of the lateral flow tests. Additionally, Sperm HY-LITER™ Express was used to microscopically confirm the absence of spermatozoa in all samples producing positive immunochromatographic results. In addition to using the quantitative proteomic assay to estimate the rate of authentic false positive results associated with lateral flow assays, this research sought to establish the correlation (or lack thereof) between absolute quantitation of seminal fluid markers and the ability to successfully generate DNA profiles. Self-collected post-coital swabs from donors engaging in barrier free vaginal intercourse with male partners over varied periods of time between 1-8 days after intercourse were collected. All samples were analyzed using the quantitative seminal fluid protein mass spectrometry assay, once again targeting SgI, SgII, and PSA. Both autosomal STR profiles (GlobalFiler™) and Y-STR profiles (Yfiler™ Plus) were subsequently generated. With regard to immunochromatographic assay false positive rates, a total of 17 false positives for semen were observed (n=150), 14 of which were consistent with PSA and 3 with Sg, for a corresponding total false positive rate of 9.3% and 2%, respectively (11.3% overall). These samples were all confirmed to be sperm negative with mass spectrometry and microscopic analysis. This data supports the use of current immunochromatographic assays for the presumptive detection of seminal fluid while also providing further support for the improved specificity of alternative serological approaches using mass spectrometry identification of biological targets. With regard to the relationship between quantitative levels of target seminal fluid peptides and the ability to generate STR profiles from vaginal swabs collected at various post coital intervals, a total of 61 post-coital samples were tested. Of these, 48 samples had a seminal fluid target greater than the limit of quantitation for the mass spectrometry assay and 26 produced an STR (n=9) and/or Y-STR (n=10) profile. A correlation between peptide quantitation and ability to generate a genetic profile was unable to be determined from this initial sample set. Overall, however, it has been demonstrated that the use of proteomic mass spectrometry for the identification of seminal fluid targets (with its enhanced sensitivity and specificity) would enable forensic practitioners to make better use of serological information during the analysis of challenging sexual assault samples.

The identification of forensic cases often includes the use of skeletal elements to assess the age-at-death of an individual. The pubic symphysis is the preferred and most often used skeletal age indicator. Standard techniques, such as the Suchey-Brooks system, require that the morphology of the pubic symphysis is visually compared to shape characteristics typical for phases with associated age intervals. As individual factors accumulate during the aging process, estimating the age-at-death for older individuals becomes increasingly more difficult. In addition, methods based on visual inspection of the bones introduce some level of subjectivity and observer-related error. This research makes use of about 100 3D laser scans of the pubic symphysis of white male skeletons with known ages-at-death, and proposes several objective, quantitative methods for shape analysis that aim to provide a surface or outline measure of the shape of the scans that minimizes the age-estimation error. The proposed methods include the use of thin plate splines, two-dimensional Fourier, wavelet and elliptic Fourier analysis, and a technique that uses the radius of a best fitting circle (in 2D) or sphere (in 3D) as a measure of the curvature of a shape. In addition some refinement and partitioning techniques were implemented. The project investigates the relationship between the exact age-at-death and the different measures produced by each method. Also included are results of applying a recently proposed computational method, the SAH-Score, to new scan data and scan partitions. As a final result, the project proposes multivariate regression models that combine the measures with highest statistical significance to minimize the age estimation error (about 12 years) and maximize the adjusted R-squared value (over 55%). Furthermore, the results are subjected to two cross-validation analysis to test for the accuracy of the models when used in practice. / A Dissertation submitted to the Department of Scientific Computing in partial fulfillment of the requirements for the degree of Doctor of Philosophy. / Fall Semester 2015. / November 3, 2015. / Includes bibliographical references. / Dennis Slice, Professor Directing Dissertation; Michael Creswell, University Representative; Bridget Algee-Hewitt, Committee Member; Peter Beerli, Committee Member; Xiaoqiang Wang, Committee Member.

Thesis (M.S.)--Boston University / This study investigated the decomposition of three pig (Sus scrofa) carcasses in the same body of water under lentic and lotic conditions and at variable depths in a temperate mixed forest in the Outdoor Research Facility (ORF) in Holliston, Massachusetts in the summer months of June and July. Data were collected on the invertebrate activity, scavenger activity, water and ambient temperature, stages ofbody decomposition, and the rate of decomposition for each set of remains. Accumulated degree days (ADD) and total body scores (TBS) were used to determine two equations, differentiated by their microhabitat, with the potential use of estimating the postmortem submergence interval (PMSI) in death investigations under similar conditions. The aquatic remains reached skeletonization in 45 days and the terrestrial control remains in 14. Terrestrial and aquatic invertebrate activity was extensive both above and below the waterline with 42 families from 17 orders collected and identified. Through the use of motion detector cameras the researcher was able to view the activities performed around the remains by a blue heron, a coyote, a raccoon, multiple black vultures, multiple turkey vultures, multiple squirrels, and multiple American bullfrogs.

Body fluid identification is an important aspect of forensic work, as it can help identify a suspect and provide information about the kind of criminal activity that took place. Blood is one of the most commonly found body fluids at a crime scene. While visually it is easily distinguishable from other fluids, an accurate method is needed to differentiate between peripheral blood and menstrual blood. This differentiation could provide critical evidence regarding consent in an alleged sexual assault. The presence of peripheral blood indicates a traumatic cause, whereas menstrual blood points towards a natural bleeding cause. Accurate detection of menstrual blood can also help with the reconstruction or corroboration of events. Menstruation is the shedding of the internal lining of the uterus that occurs on a monthly basis in women of a reproductive age group. Menstrual blood is different in composition from the peripheral blood flowing through arteries and veins. It consists of a mixture of vaginal and cervical secretions, epithelial cells, debris from the endometrial lining, blood and fibrinolytic products. The fibrinolytic products are associated with the prevention of blood clot formation. Several methods have been researched and used for the detection of menstrual blood. These include microscopy, identification of the lactate dehydrogenase isoenzyme, detection of fibrinolytic products, and profiling of messenger ribonucleic acid (mRNA) and micro RNA (miRNA). Even though menstrual blood is encountered at crime scenes, a reliable routine procedure for its identification has not yet been incorporated in forensic laboratories. In this study, four methods of detection of menstrual blood were evaluated and compared with each other regarding efficacy. These methods are the LGC ParaDNA® Body Fluid ID Test, SERATEC® PMB Test, DIMERTEST® Latex Assay and Microscopic methods using Lugol’s Iodine and Dane’s staining method. The LGC ParaDNA® Body Fluid ID Test identifies menstrual blood by detecting the mRNA marker MMP10. The SERATEC® PMB Test and DIMERTEST® Latex Assay both detect D-dimers present in menstrual blood. In addition, the SERATEC® PMB Test can detect the presence of peripheral blood. Microscopic identification is performed by identifying vaginal epithelial cells present in the menstrual blood. Menstrual blood samples were self-collected from six anonymous donors on three consecutive days of menses on either a cotton swatch or a cotton swab. Samples from the earliest day were tested in triplicate using the first three methods. For the fourth method, Lugol’s Iodine and Dane’s stain were applied to various cell types to test the utility of the stains. The ParaDNA® Body Fluid ID Test, SERATEC® PMB test and the DIMERTEST® Latex Assay all show promise for the detection of menstrual blood in forensic samples. None of the tests showed a cross reactivity to the other body fluids tested, but some ParaDNA® and DIMERTEST® samples yielded a false negative result for menstrual blood or peripheral blood. The SERATEC® PMB Test outperformed the other methods, both in sensitivity and accuracy. It was accurate for all samples, with a short run time and minimal training required. Microscopic detection of menstrual blood via detection of vaginal epithelial cells could not be accurately investigated as Dane’s staining method could not be reproduced and the presence of blood obscured the results for the Lugol’s method.

Cusirisna Cave was discovered in the 1870s by Dr. Earl Flint, an explorer for the Harvard Peabody Musuem. The human remains and artifacts found in the cave were collected and sent to the museum, where they have remained since, unanalyzed. In December 2011, Dr. Clifford T. Brown and I analyzed the osteological material and artifacts because we thought they might be related to the Preclassic cave complexes of neighboring Honduras, an idea originally suggested by Dr. James Brady. I analyzed the human remains while Dr. Brown studied the artifacts. This thesis presents the results of the analyses and compare the findings to other mortuary complexes in Mesoamerica. Despite the paucity of material culture, information regarding context, and the small sample size, I propose Cusirisna as a place of exceptional ritual importance. This project adds to our understanding of cave bioarchaeology, mortuary practices in Mesoamerica, and the prehistory of Nicaragua. / by Kendra L. Philmon. / Thesis (M.A.)--Florida Atlantic University, 2012. / Includes bibliography. / Mode of access: World Wide Web. / System requirements: Adobe Reader.

Trasology and its use in crime investigation Abstract Title of this thesis is Trasology and its use in crime investigation. This thesis focus on trasology which is discipline of forensic science examining foot traces, traces of other body parts and traces of vehicles. The objective of this thesis is to provide its reader basic summary about this discipline of forensic science and methods used in this discipline especialy aplication of biomechanincs in trasology and height estimation from foot prints dimensions. This thesis is divided into eleven chapters. First chapter after introducing chapter is chapter about history of trasology, folowing chapters describe individual groups of trasological traces. Folowing chapters focus on methods of detecting and capturing trasological traces. Chapters number seven and eight are about methods of forensic examination of trasological traces. Main part of this thesis consist of chapter about aplication of biomechanics in trasology and experimental chapter presenting results of comparison of methods of height estimetion from foot prints dimensions. Biomechanics is used in trasology mainly for estimation of height from traces of various body parts. In the experimental chapter you can find comparison of six methods of height estimation from length and breadth of foot traces....

Forensic analysis of deoxyribonucleic acid (DNA) collected from sexual assault evidence is a multi-step process that requires a great amount of time and resources. A large percentage of samples are mixtures containing DNA from a major female contributor and at least one minor male contributor. The amount of male DNA present is often much less than that of the female, making it difficult to achieve a full short-tandem repeat (STR) profile for identification purposes. The current method employed by many forensic laboratories to separate sperm DNA from non-sperm DNA is the differential extraction. Although a robust and reliable method when applied to liquid samples, the procedure has failed to evolve significantly since first developed.1,2 Between the time it has been collected and tested, sexual assault evidence becomes dried and aged, contributing to the potential loss and degradation of already low amounts of DNA and increasing the likelihood of an incomplete profile.2 This study tests the effectiveness of a combination of enzymes to release DNA from sperm using a variety of substrates. Although this method extracted greater amounts of male DNA than the traditional Qiagen® extraction, further research is necessary to determine if the application of this new method can improve or eventually replace the current procedures. / 2018-06-16T00:00:00Z

Fingerprints in blood indicate a threshold of violence has been surpassed in crime scenarios - making the crime resolution more urgent. There exist multiple processes that enhance a blood fingerprint in its original position, or in-situ, with reliability so that an image can be obtained. However, blood fingerprint evidence that cannot directly be transported to a laboratory for further analysis, due to the size or mobility of the substrate, calls for portability. In 2010 Zar-Pro Fluorescent Blood Lifting Strips were patented by Jessica Zarate as a "fluorogenic method for lifting, enhancing, and preserving blood impression evidence". The lifted prints are also inherently fluorescent to further increase enhancement and contrast of the print. There are currently no studies comparing Zar-Pro results with the results of other laboratory enhancement methods. This experiment compared Zar-Pro to other non-portable and frequently used alternatives - blood peak absorption and Hungarian Red enhancement to determine if Zar-Pro gives better blood fingerprint enhancement results than other non-portable alternatives - ALS visualization and Hungarian Red enhancement. In this study, Zar-Pro methods produced more reliable and reproducible results over the Hungarian Red and blood peak absorption methods on white and black ceramic tile. From this study, one can also conclude that ALS peak absorption is better suited for the location of blood prints on a light-colored item of evidence, rather than an enhancement method of blood prints.

Several factors that influence the rate of human decomposition have been described in the literature, including temperature, access by insects, humidity and rainfall1. These environmental factors, as well as purge fluid released during decomposition2, can interact with evidence deposited on the clothing of a deceased individual. The present research assessed how these combined factors affect the detection and identification of blood and semen evidence, as well as subsequent DNA analysis. A 35-45 pound (lb) feeder pig (post-mortem interval (PMI) < 3 hours) was placed on a grassy area within the Boston University Outdoor Research Facility for a period of 22 days or 364.3 accumulated degree days (ADD) during late spring, with the temperature averaging 16.5 oC. Aliquots of 30 μl of either human blood or semen were pipetted onto 1 inch by 1 inch sections of a 95% cotton t-shirt. Twenty-two samples of each type were placed on top of and underneath the pig, as well as a similarly weighted bag of sand (control). One bloodstain and one semen stain were collected each day for a period of 22 days from each location, yielding 8 samples per day. Each sample was analyzed within 30 hours of collection. The blood samples beneath the control showed that environmental factors influenced the results of testing. Rain caused dilution and diffusion of the bloodstains and the color of the stains changed from red-brown to green-yellow. Kastle-Meyer (KM) testing was positive for all samples and ABAcard® HemaTrace® testing was positive for 14 of 22 samples, with the negative results occurring during days 12 - 21. Two stains that were negative at 10 minutes turned positive shortly thereafter, suggesting that a longer development time may be required for compromised samples. The blood samples placed beneath the pig yielded positive KM results on all 22 days and positive HemaTrace® results through day 10. All bloodstains placed on top of the pig and control yielded positive KM and HemaTrace® results. The blood samples from on top of the pig and control yielded full short tandem repeat (STR) profiles for each of the four days of testing (days 1, 8, 13 and 20). The blood samples from beneath the pig and control yielded full profiles on day 1 only. The three subsequent days of testing yielded a maximum of three alleles per sample, with the majority of samples failing to provide any profile at all. Semen samples from beneath the control began to show a decrease in fluorescence using an alternate light source (ALS) by day 3, and some areas of fluorescence occurred in a different location, indicating that the soluble components had diffused outward from the original region of deposition (ORD). Results for acid phosphatase (AP) and ABAcard® p-30 were mostly positive through day 16. By day 17, the ORD no longer fluoresced or yielded positive AP or p-30 results. With the exception of day 10, sperm were identified on all samples. Semen results from beneath the pig showed that even on day 1, the ORD was only weakly fluorescent and by day 4, fluorescent regions began appearing outside of the ORD. These outlying regions of fluorescence yielded positive results with AP Spot and p-30 testing, but showed few or no spermatozoa when examined microscopically. As the days passed, the ORD were no longer fluorescent and AP mapping and p-30 testing yielded negative results; however, spermatozoa could still be identified in almost all of the ORD through day 22. Semen samples collected from on top of the control showed that semen stains retained fluorescence and tested positive for AP, spermatozoa and p-30 through 22 days of testing. Semen samples collected from on top of the pig yielded similar results until day 16, when the fluorescence began to fade and AP testing did not yield traditional color changes associated with a positive result. By day 18, fluorescence was no longer visible with an ALS at 450 nm or 495 nm, however, UV light yielded positive fluorescence when used during days 19-21. Spermatozoa and p-30 were identified on samples saturated with products of decomposition, even when presumptive screening techniques were negative (450-495 nm) or showed an altered appearance (AP). Semen samples from within the ORD yielded full 16 loci profiles from beneath the pig and both on top of and beneath the control on each of the four days of testing. The samples collected from on top of the pig yielded full profiles on days 1, 6 and 14 and partial profiles on day 20. Samples from beneath the pig on days 6 and 14, which had positive presumptive results outside of the ORD, were also amplified, but failed to yield a profile.