Knowledge of the relationships between nitrogen fertilization and nitrogen availability is needed to guide plans for providing optimum tree growth by silvicultural operations. PART 1. Detailed studies of the mineralization of urea were made by laboratory incubation of black spruce humus samples. These studies form a part of an experiment in which urea is used for the first time to fertilize a soil supporting a black spruce stand. Urea seems to be a very good fertilizer because it is readily transformed to an available form of nitrogen. It is however doubtful that a nitrogen fertilization only could quickly and greatly affect the humus fertility. PART 2. Total number and number of ureolytic bacteria and fungi were determined at time intervals during a laboratory incubation of black sprucc humus samples. Such a quantitative and qualitative study of the microbial population of a raw humus had never been made. The potential for ureolysis is enormous because this humus contains an abundance of ureolyzing microorganisms Indeed, counts in excess of 100 millions per gram are not uncommon.

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No description available.

No description available.

The objective of this study was to examine the relationships between Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) foliar nutrient status and forest floor chemical properties. Foliar nutrient analysis data were collected on 27, 50- to 140-year-old Douglas-fir stands, for which the forest floor chemical properties had previously been analysed. The 27 sites encompassed a broad geographical area within the Coastal Western Hemlock Biogeoclimatic Zone. Foliar samples were individually analysed for total S, N, P, K, Ca, Mg, B, Cu, Zn, Fe, Al, Mn and for AFe (active iron). Four foliar nutrient ratios (N/S, N/P, K/Ca, Ca/Mg) were additionally calculated. As the foliar sampling intensity was often below that recommended in the provincial sampling guidelines, the precision of the within-site foliar nutrient variable mean values was examined for the lowest sampling intensity used in this study (n=8). Foliar nutrient and ratio variances from 6 of the 27 sites, for which 15 trees per site had been sampled, were used as population variance estimates. A sampling intensity of n=8 was found to be sufficiently large to provide foliar nutrient variable mean estimates with an allowable error of 20% (∝ =0.5) for all but the following foliar nutrients and ratios: B, Cu, Ca/Mg and K/Ca. Two combinations of forest floor horizons were examined for possible relationships with foliar nutrient variables: L plus F and L plus F plus H. These two combinations of forest floor horizons were referred to as the LF and the LFH layers. The two criteria used to select forest floor chemical properties are as follows: (1) the precision of the forest floor chemical property site estimate was less than or equal to 20% (∝=0.5) for the field sampling intensity used, or (2) the forest floor chemical property was an established measure of nutrient availabilty. The 12 LF and 16 LFH chemical properties selected included: LF and LFH: pH (CaCl₂), total-S, -N, -P, -Cu, -Zn, -Mg, -K, -C, Min-N (anaerobic mineralizable N), KC1-N (KC1 extractable N, A (lipids), and LFH (only): exK, exCa, exMg (1M NH₄OAC exchangeable bases) and extP (Bray P₁ extractable P). Simple linear relationships between foliar nutrient variables and LF and LFH chemical properties were initially examined. Strong negative associations were found between foliar S and certain forest floor chemical properties, namely LF: pH, LMg and LFH: pH, LMg, LexMg, LexCa, LexK (here and following, L signifies the natural logarithm). It was hypothesized that these relationships reflected the effect of the different sources of plant available sulphur predominant on the higher (17 sites) and lower (10 sites) forest floor pH sites. Organic sulphur mineralization and mineral weathering were suggested as the major sources of plant available sulphur for the higher forest floor pH sites; anthropogenic atmospheric sulphur was suggested as the major source of plant available sulphur for the lower forest floor pH sites. The efficacy of forest floor (LF and LFH) chemical properties for predicting and classifying foliar nutrient status was examined through the use of multiple regression and discriminant analysis, respectively. These analyses were performed for the four foliar nutrient variables for which a range in sufficiency and possible deficiency (actual or inducible) was indicated for the stands sampled: foliar N, S, AFe and N/S. One regression model of potential predictive value was found for foliar LS. Together, LFH pH and LFH LCu statistically accounted for 79% of the observed variation in foliar LS. Several LF and LFH chemical properties were able to reliably classify stands into the following total foliar S groupings: sufficient and deficient (actual or inducible). LF LMg and LF A provided the best classification of stands into the groupings: S sufficiency and N-inducible S deficiency, as defined by foliar N/S. Three LFH chemical properties: P, S, and LexK provided the best classification of stands in terms of adequate and slight to severely deficient foliar N status. LF and LFH chemical properties were only poorly able to classify foliar AFe status. It was concluded that the relationships found in this study could not be extrapolated beyond the present study, due to the unforeseen, yet probable, influence of slightly acidic precipitation on both nutrient cycling and foliar nutrient status for 10 of the 27 stands examined. / Land and Food Systems, Faculty of / Graduate

No description available.

Although the physiological nitrogen demand of the soil microbial biomass is a major determinant of N mineralization in forest soils, the exact nature of the relationship is unclear. This study investigated the relationships between a respiration-based indicator of microbial physiological N demand (NIR) and N availability in forest soils. NIR was found to correlate significantly with net mineralized N in the field and annual foliar litterfall N fluxes. In a laboratory incubation, NIR was shown to be sensitive to changes in soil available C and N pools. These results demonstrated that microbial physiological N demand is determined by relative availabilities of labile C and N, and that it is significantly related to N cycling in forest soils. Results from a seasonal study of a forested watershed suggest that nutrient availability determined tree production and soil C availability, which in turn determined microbial physiological N demand and nitrogen dynamics in the forest.

No description available.

Extensive areas of Sitka spruce plantations established during the last 15 years on the CH-phase of the salal-moss ecosystem association on northern Vancouver Island are presently exhibiting symptoms of severe chlorosis and growth check. Investigations into the poor performance of these plantations are described in this thesis. Comparisons of the soil nutrient levels between this poorly productive CH-phase and the adjacent highly productive HA-phase were made. In addition, fertilization screening trials were established in order to identify possible limiting nutrients and determine the potential responsiveness of Sitka spruce to fertilization. Soil samples were collected over an age sequence of cutovers from both phases in order to compare differences in the soil nutrient status and to determine the effects of time since harvesting on N mineralization rates. Significantly greater amounts of total and available P and total, KC1 extractable, and mineralizable N (p=.01) were found in the mineral soil and humus of the HA-phase. The mineral soil of the HA-phase also had significantly higher (p=.05) concentrations of exchangeable K. In addition, this phase had a significantly lower (p=.01) C/N ratio. Although significant differences were noted in the mineral nutrient composition of the two phases, in all cases (with the possible exception of mineralizable N), the absolute differences between the two phases did not appear large enough to account for the dramatic differences in productivity. Harvesting plus slashburning appears to have had little effect on the rates of N mineralization in the humus or mineral soil of the HA-phase. However, in the CH-phase harvesting plus slashburning has had a significant influence on the mineralization rates of the humus but not of the mineral soil. Harvesting initially resulted in a significant (p=.05) increase in the mineralization rates, however, within five years they returned to levels comparable to that of the control. Fertilization screening trials were established in five Sitka spruce plantations ranging in age from 8 to 14 years. All plantations were growing on the CH-phase and were exhibiting symptoms of chlorosis and growth check. Changes in the first-year needle dry-weight, needle nutrient composition, and leader growth were monitored. Treatments for these trials consisted of a N, P, K factorial experiment, and one separate treatment of a complete nutrient mixture. Each treatment was replicated seven times in each of the five plantations. N or N plus K fertilization resulted in a 10% increase in needle dry-weight and a 25 to 30% increase in first-year leader growth response over that of the control. P fertilization had a strong synergistic effect and when added in combination with N or N plus K resulted in a needle dry-weight increase of 40 to 41% and a leader growth increase of 78 to 83%. A limited sample of naturally occurring hemlock in some of the plots exhibited similar trends, although leader growth increases were somewhat greater. Fertilization with P and K, alone or together, had no effect on leader growth or needle weight despite the severe deficiencies of these two elements. However, P had a positive influence on the uptake of N, K, Ca and Mg, whereas K positively affected Ca and depressed Mg. After N fertilization, foliar N concentrations increased dramatically, ranging from a mean of 3.2% for the lowest (200 kg N/ha) application rate to 4.1% for the highest (400 kg N/ha) application rate. The high foliar N concentrations had a significant negative influence on foliar K concentrations. This decline in K concentrations could only partially be attributed to a growth dilution effect and occurred whether or not K was added. A similar effect on P, Ca or Mg was not noted. The high N concentrations also appeared to have had a negative influence on both needle weight and leader growth as there was a tendency for the response of both to decline as N concentrations increased. There were significant differences in both leader growth and needle weight responses between some of the plantations, but treatments receiving both N and P fertilizers consistently gave the greatest responses. The individual treatment, however, which gave the greatest and most consistent overall response was that containing all macro- and micro-nutrients. Results from this study indicate that the poor performance of Sitka spruce plantations established on the CH-phase of the salal-moss ecosystem is partially due to a severe N and P deficiency. These deficiencies are associated with the complete invasion of these cutovers by salal. It has also been shown that the Sitka spruce in these plantations would be highly responsive to N and P fertilization. Although K does not appear to limit growth and no apparent benefits were gained from K fertilization, a severe N induced K deficiency is likely. / Forestry, Faculty of / Graduate

Western hemlock seedlings were grown for 8 months in a greenhouse with fertilizer amendments of N, P, N+P, and lime. Humus material was used as a growth medium. It was collected from low-productive sites identified by Lewis (1985) as the cedar-hemlock phase of the Thuja plicata - Tsuga heterophylla - Gautheria shallon - Rhytidiadelphusloreus ecosystem association unit designated as S1CH , on his ecosystem maps of Tree Farm Licences 6 and 25, which are located on northern Vancouver Island. A combination of 100 kg/ha N and 150 kg/ha P produced the most favourable seedling growth and foliar nutrient status. N or P added separately at these rates also gave superior seedling growth compared to the control. Neither lime, nor high rates of N (at 300 kg/ha with or without P) increased seedling growth. High N additions also restricted root development. N fertilization reduced foliar P concentrations, and P addition decreased foliar N concentrations. Foliar Mg concentrations increased when P fertilizers were added. N and/or P additions caused a decrease in foliar B concentrations. A combination of N and P increased foliar Zn concentrations and S, but reduced Mn absorption. Foliar R was inversely related to seedling productivity. Management recommendations for the S1CH sites include fertilizing with P, and increasing available N either by cultivation to increase mineralization or by low rates of N fertilization / Land and Food Systems, Faculty of / Graduate