To accommodate for human population growth along the Texas I-35 corridor, land is becoming increasingly urban and decreasingly pervious, modifying the infiltration and runoff rates in the Edwards Aquifer, especially to its spring fed Upper San Marcos River (USMR). Contaminants like heavy metals and organic chemicals can accumulate on impervious surfaces and with runoff, enter into the USMR at potentially harmful levels. The objective of this study was to determine how the population of an endangered Edwards Aquifer species, the fountain darter (Etheostoma fonticola), might respond to potential water quality changes associated with urbanization. I developed a stochastic, sex and stage-structured population dynamics simulation model that represents the relationships between urbanization, springflow variations, contamination levels, and natural history of the fountain darter. Future fountain darter population trends (2008-2040) were simulated under 10 treatments of nine scenarios. A simulation scenario (n=50) corresponded to one of three variations of springflow (random, high and low flow) and one of three variations in percentage of runoff entering the river (100, 50 or 30). The 10 treatments were variations on water quality: uncontaminated (1), contaminated by Cu (2), Zn (3), Cd (4), Cr (5), polycyclic aromatic hydrocarbons (PAH) (7), bifenthrin (8), carbaryl (9) and dicamba (10) and an additive affect of Cu, Cr, Cd, and Zn (6). Simulating ideal conditions, the average darter population from 2008-2040 was 54155+2969 (mean+SE) individuals. Contaminant treatments that caused a significant (p<0.001) decline in the population by 2040 under 100% runoff conditions were the all metal (650 plus/minus 640), Cu (3141 plus/minus 265), PAH (4621 plus/minus 475), Zn (6169 plus/minus 5406), and Cd (27987 plus/minus 6751) scenarios. With 50% runoff, the all metals (15740 plus/minus 5455), Cu (16815 plus/minus 6263), PAH (19675 plus/minus 995), and Zn (15585 plus/minus 3097) treatments simulated significantly lower populations (p less than 0.001). At 30% runoff, Cu (23976 plus/minus 6787), the all metal (25853 plus/minus 7404) and PAH (28167 plus/minus 1194) treatments decreased the population significantly (p less than 0.001). Over all scenarios, copper, zinc and PAHs caused >50% decline in the population. Assuming 100% or 50% of all San Marcos sub-basin runoff is directly entering USMR, there could currently be levels of Cu, Zn, and PAHs higher than what darters can withstand.

Between July 2009 and October 2011, a new habitat was found for a rarely reported freshwater dinoflagellate species, Thompsodinium intermedium - Comal Springs (Comal County), Texas. In 2011, diel in-situ monitoring in monospecific blooms of this species revealed previously undetected negative impacts on endangered species habitat availability associated with conditions of low flow levels, recorded at the U.S. Geological Survey gage # 08169000 on Texas Commission on Environmental Quality river segment 1811 station 12655. During a period of low springflow in the summer of 2011, late afternoon and early morning measurements of dissolved oxygen and temperature and presence of dinoflagellate blooms were monitored at six sites. Significant differences in diel fluctuations were found in all of these parameters among sites with and without the planktonic blooms. These fluctuations increased risk of hypoxia and hyperthermia conditions at sites of planktonic bloom events. Arrays of in-situ continuous monitoring temperature/light probes were used inside and outside of blooms. Wildlife and human health implications are that hypoxia and hyperthermia are known to promote conditions favorable to harmful microbes which may be transported from springs to coastal bays. In-situ data demonstrated that T. intermedium blooms, hypoxia, and hyperthermia occurred in the upper Comal headwaters. These natural environmental stressors may be avoidable if adequate springflows are maintained to buffer against these impacts.