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published_or_final_version / abstract / Biological Sciences / Doctoral / Doctor of Philosophy

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by Siu-wang Lau. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2000. / Includes bibliographical references (leaves 121-127). / Abstracts in English and Chinese. / Contents page / Acknowledgement --- p.ii / Abstract --- p.iii / Abbreviations --- p.v / Contents --- p.vi / Introduction --- p.1 / Chapter Part I / Chapter 1 --- Principle of Fourier transform infrared spectroscopy (FTIR) --- p.9 / Chapter 2 --- Experimental --- p.15 / Chapter 2.1 --- Method of calibration curve for FTIR --- p.15 / Chapter 2.2 --- Method of standard addition for FTIR 18 、 / Chapter 3 --- Results --- p.19 / Chapter 3.1 --- Results of the method of calibration curve for FTIR --- p.26 / Chapter 3.2 --- Results of the method of standard addition for FTIR --- p.34 / Chapter 4 --- Discussion --- p.37 / Chapter 5 --- Interferences between the major components when two standard chemicals were considered each time (mixture of two standard compounds) --- p.41 / Chapter 5.1 --- Introduction --- p.41 / Chapter 5.2 --- Procedures --- p.43 / Chapter 5.3 --- Results --- p.47 / Chapter 5.4 --- Discussion --- p.80 / Chapter 6 --- Interferences between the major components when four standard chemicals were considered each time (mixture of four standard compounds) --- p.83 / Chapter 6.1 --- Introduction --- p.83 / Chapter 6.2 --- Experimental --- p.83 / Chapter 6.3 --- Results --- p.85 / Chapter 6.4 --- Conclusion --- p.89 / Chapter 7 --- Discussion --- p.90 / Chapter Part II / Chapter 1 --- Introduction --- p.92 / Chapter 2 --- Principle of flame emission spectroscopy (FES) --- p.92 / Chapter 3 --- Introduction of the analysis of total calcium by FES --- p.94 / Chapter 4 --- Experimental --- p.104 / Chapter 4.1 --- Methods of digestion --- p.104 / Chapter 4.2 --- Methods of oxidation --- p.105 / Chapter 4.3 --- Calibration of standard --- p.106 / Chapter 5 --- Results --- p.107 / Chapter 6 --- Discussion --- p.112 / Chapter Part III / Chapter 1 --- Estimation of total calcium by FTIR --- p.113 / Chapter 2 --- Experimental --- p.113 / Chapter 3 --- Results --- p.115 / Chapter 4 --- Discussion --- p.119 / Chapter Part IV --- Conclusion --- p.120 / Chapter Part V --- References --- p.121 / Appendices / Appendix 1 Example of the calculation of analyte content by the method of calibration curve --- p.128 / Appendix 2 Examples of the calculations of the analytes by the method of standard addition --- p.130 / Appendix 3 IR spectrum of a mixture of four standard chemicals --- p.132 / Appendix 4 IR spectrum of a sample --- p.133

Lo, Yu Ching. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 79-82). / Abstracts in English and Chinese. / Acknowledgements --- p.i / Abbreviations --- p.ii / Abstract --- p.iii / Table of Contents --- p.v / Chapter Chapter 1 --- Introduction / Chapter 1.1 --- Basic Theory of FTIR --- p.1 / Chapter 1.1.1 --- Infrared Spectroscopy --- p.1 / Chapter 1.1.2 --- Dispersive Infrared Spectrometer --- p.2 / Chapter 1.1.3 --- Fourier Transform Infrared Spectrometer (FTIR) --- p.3 / Chapter 1.1.4 --- Advantages of FTIR --- p.5 / Chapter 1.1.4.1 --- Multiplex advantage --- p.5 / Chapter 1.1.4.2 --- Throughput advantage --- p.5 / Chapter 1.2 --- Traditional Chinese Medicine (TCM) --- p.6 / Chapter 1.3 --- Identification of Traditional Chinese Herbal Medicine --- p.8 / Chapter 1.3.1 --- Traditional method for the identification of TCM --- p.8 / Chapter 1.3.2 --- Instrumental method for the identification of TCM --- p.9 / Chapter 1.3.2.1 --- Identification of TCM using fingerprint method --- p.9 / Chapter 1.3.3 --- Identification of TCM using FTIR fingerprint method --- p.10 / Chapter 1.4 --- Objective --- p.11 / Chapter Chapter 2 --- Experimental / Chapter 2.1 --- Outline of the Method --- p.12 / Chapter 2.2 --- Reagents and Glassware --- p.13 / Chapter 2.3 --- Instrumentation --- p.13 / Chapter 2.4 --- Library Search Program --- p.13 / Chapter 2.5 --- Samples --- p.14 / Chapter 2.6 --- Sample Pretreatment --- p.16 / Chapter 2.7 --- Extraction of Ingredients --- p.16 / Chapter 2.8 --- Preparation of KBr Pellet --- p.17 / Chapter 2.9 --- IR Spectrum Measurement --- p.17 / Chapter 2.10 --- Data Processing --- p.18 / Chapter 2.11 --- IR Databases --- p.18 / Chapter 2.12 --- Reproducibility of Extraction --- p.29 / Chapter Chapter 3 --- Application of Library Search Techniques - Results and Disscussion / Chapter 3.1 --- Introduction --- p.31 / Chapter 3.2 --- Euclidean Search Method --- p.32 / Chapter 3.2.1 --- Similarly score of reference spectra --- p.32 / Chapter 3.2.2 --- Similarity score of known sample spectra --- p.37 / Chapter 3.3 --- Soft Independent Modeling of Class Analogy (SIMCA) --- p.45 / Chapter 3.3.1 --- Verification Diagnostic Report of the Reference flowers --- p.47 / Chapter 3.3.2 --- Classification of Flowers --- p.51 / Chapter 3.4 --- Performance Limitation --- p.75 / Chapter 3.4.1 --- Euclidean Search Method --- p.75 / Chapter 3.4.2 --- SIMCA --- p.75 / Chapter 3.5 --- Conclusion --- p.77 / References --- p.79