This work was performed as part of a major research project into the evaluation of the ecology of lake dwelling Brown Trout, Salmo trutta L. using ultrasonic biotelemetry techniques. The supplementary research results. leading up to and after the execution of a program of experiments involving the telemetry of feeding and ventilatory rhythms are described: 1. The presence of red (slow) fibres in the adductor mandibulae muscle of Brown Trout was confirmed to be as previously described in the Rainbow Trout, Sälmo gairdneri Richardson and other salmonids. 2. By electromyographic (EMG) and pharmacological means, the red fibres in the a. mandibulae were shown to be active during ventilation and the mosaic fibres comprising the bulk of the muscle were recruited during more dynamic events such as feeding and coughing. Observations were made on the innervation of the red fibres. 3. Comparative investigations made at sea on large deep sea Squaloid and Galeoid sharks (which have a simple adductor muscle like the Trout) showed an identical functional differentiation as obtained in the Trout. 4. The presence of a migratory 'pace setter potential' was found for the first time in Fish. Its use as an indicator of feeding activity by telemetry was rejected on practical grounds. ýýY NO 5. An ultrasonic transmitter was developed to telemeter an analogue of the adductor mandibulae EMG from wild Brown Trout, using a novel electrode design. Four fish were so equipped and released into Airthrey Loch, University of Stirling and tracked for up to 24 hours (following a 24 hr allowance for post-anaesthetic recovery). Feeding and ventilatory periodicity, linear and angular movement patterns and photoperiod were intercorrelated. Angle of turn and subsequent step length were positively correlated and feeding activity was marked by a preference for dextral turning. 'Area restricted searching' and 'area avoided searching' were the probable causes of the movement patterns seen in this and previous investigations at Airthrey Loch. A depth preference and orientation of the fish to topography was demonstrated. Following analysis of the angle of turn and step length data, it was concluded that the larger transmitter package and more severe surgery materially affected the fishes' behaviour relative to data previously obtained at Airthrey Loch using smaller transmitters. 6. Due to difficulties experienced in 5 above due to an unsuspected effect on the a. mandibulae EMG detectable up to 24 hrs post-anaesthesia, a frequency analysis was made of the a. mandibulae EMG of the Brown Trout and several other species. This disclosed that the EMG from red fibres has a frequency spectrum considerably lower than that of 'standard' mammalian muscle. The progressive failure of the EMG transmitter with time was due to a combination of the anaesthetic effect and the frequency spectrum relative to certain design features. In the light of these observations, subsequent designs of the EMG transmitter were able to take this into account.

M.Sc. (Zoology) / An investigation was made into the ecology of Barbus brevipinnis and Opsaridium zambezense in the Incomati River System in the Eastern Transvaal. Special attention was given to factors which may threaten the existence of both species. New information was collected on aspects concerning their length:mass relationship, condition, fecundity, feeding habits as weIl as parasites. Measurements were made of the standard, fork and total lengths of both species, which in each case was related to the specific mass of the fish. Both species are, according to the information obtained, capable of successive spawning over an extended period during the breeding season. This implies that the evaluation for fecundity according to standard procedures may in both species be conservative in terms of estimates of the total numbers of eggs produced during the breeding season. Indications are, however, that of Zambezense may produce relatively low numbers of eggs and this as weIl as certain environmental factors, may in combination explain the relative scarcity of this fish in the Inkomati River System. Environmental pollution may further complicate this issue. One of the main objectives of this study was to investigate the habitat requirements of both species. In doing so, both species can now be better defined in terms of certain biological requirements as welI as environmental conditions under which they occur. It is hoped that this information may contribute towards a better understanding of the environmental conservation status of both species. The scarcity collection of studies into including GSI. of both species prohibited the large scale specimens normally required to enable intensive factors such as feeding, growth and breeding, It was therefore not possible during this study to obtain an accurate assessment of parameters such as fecundity, which should be investigated in more detail if and when material on both species becomes available. Recommendations are made concerning morphometric and electrophoretic investigations of both species in order to evaluate their species status more accurately. It is suggested that the habitats where B.brevipinnis and O.zambezense occur be closely monitored in future to observe any further deterioration which may be caused by agricultural and mining activities. It is recommended that because of the relative scarcity of Zambezense in the Inkomati River System, and to the apparent sensitivity of this species to environmental disturbance of its habitat, its conservation status be changed from indeterminate scarce to vulnerable.

D.Sc. (Zoology) / Please refer to full text to view abstract

Distribution patterns and levels of genetic diversity in extant taxa are a product of complex palaeogeographic processes and climatic oscillations as well as the species’ intrinsic ecological adaptations. The Cape Floristic Region of South Africa presents a unique system for studying the processes that promote species diversification and distribution patterns. This region has a high degree of endemism of both terrestrial and aquatic biota and is clearly isolated from neighbouring areas by the Cape Fold Mountains and the Great Escarpment. The objective of this study was to firstly examine the ecology of freshwater fishes belonging to the genera Galaxias, Pseudobarbus and Sandelia in the south-western CFR. This was followed by an assessment of the genetic diversity of these taxa. Unique lineages were identified and their distribution was mapped. The work aimed to explore the role of the region’s complex palaeogeographic and climatic history as well as the role of the species’ ecological adaptations in driving lineage diversification and shaping contemporary distribution patterns. The four main components of the study can be summarised as follows: 1. Habitat associations of three widely distributed lineages of Galaxias zebratus Pseudobarbus burchelli and Sandelia capensis were evaluated at multiple localities in minimally disturbed mountain tributaries of the Breede, Duiwenhoks and Goukou River systems. The lineages have distinct habitat associations which were related to differences in their morphological traits. The slender-bodied Galaxias ‘nebula’ and the fusiform-shaped Pseudobarbus ‘Breede’ are capable of exploiting upper reaches with faster water velocity. By contrast, the laterally compressed Sandelia ‘eastern’ is restricted to lower reaches, making this lineage more susceptible to a wide array of impacts. 2. A recently discovered lineage of Galaxias zebratus, (Galaxias ‘nebula’), was found to be capable of tolerating emersion for a prolonged period of time. This is the first time that such capabilities have been documented in an African galaxiid. These adaptations have implications for the interpretation of Galaxias ‘nebula’s wide distribution range. 3. The phylogeography of Galaxias ‘nebula’ across its entire distribution range was investigated using two mitochondrial genes (cytochrome c oxidase subunit I (COI) and cytochrome b (cyt b)). This lineage has a complex evolutionary history that was influenced by both intrinsic and extrinsic factors. Rare events such as episodic drainage connections during Pleistocene and Holocene pluvial periods, possibly augmented by river confluences during periods of lower sea-levels and river capture events seem to be the most credible explanation for the extensive contemporary distribution and the relatively shallow genetic divergence between different river systems. 4. Mitochondrial cyt b sequences were used (i) to assess genetic diversity in G. zebratus, P. burchelli and S. capensis from the south-western CFR and (ii) to determine the roles of intrinsic ecological adaptations and extrinsic landscape and climatic changes in promoting genetic diversification and shaping present day distribution patterns of lineages in the three taxa. Marine incursions during periods of major sea-level transgressions are proposed to have isolated populations in upland refugia, thereby driving allopatric divergence in these species. Subsequent connections of rivers during wetter periods and lower sea-levels are proposed to have facilitated post-speciation dispersal of lineages to attain present day distribution patterns. While detailed morphological studies and further genetic analysis are needed to substantiate the taxonomic status of the newly discovered lineages of Galaxias zebratus, Pseudobarbus burchelli and Sandelia capensis, results of the present study indicate that the south-western CFR represents a previously unrecognised centre of freshwater fish diversity and microendemism in the broader Cape Floristic Region. Accurate identification of lineages and comprehensive mapping of their distribution is a fundamental pre-requisite for ecological studies, assessing conservation status and implementation of appropriate conservation measures.

Thesis (MSc)--University of Stellenbosch, 2002. / ENGLISH ABSTRACT: In this study, the Fish Assemblage Integrity Index (FAll) was applied on three rivers within the southwestern Cape. This index uses fish as indicators of biological aquatic integrity and is based on indigenous species expected to be present in biological fish habitats. Fish integrity classes were calculated for each of the sites in the three rivers studied. Sites 2 and 4 within the Lourens River were rated as Class C, whereas sites 1 and 3 were rated as Class F and Class D respectively. Sites 1, 2 and 4 within the Palmiet River were rated Class F, whereas sites 3 and 5 were rated as Class E and Class D respectively. Site 1 within the Hout Bay River was rated as a Class F site, in addition to sites 2 and 3 been rated as Class A. It is however, suggested that the FAll needs to be adjusted to accommodate the general low species richness experienced in the southwestern Cape. In addition to the FAll been applied, the effects of long-term exposure to subtle water quality changes associated with human activities, specifically potential estrogenic compounds in fish from the Lourens River were also investigated. The production of the yolk precursor lipoprotein complex, vitellogenin (Vtg) produced in the liver under estrogen control was employed as biomarker for environmental estrogen exposure. Male fish from the Lourens River were studied using SDS-PAGE gel electrophoresis. Results indicated that 60% of male fish showed the presence of Vtg in their plasma. Abnormal gonad morphology in male and female fish were also assessed using standard histological procedures. Results from this study indicated no observed abnormalities in either male or female gonads. The immediate presence of endocrine disrupters with estrogen activity was investigated by screening water samples from the Lourens, Palmiet and Hout Bay Rivers for estrogen activity. Results indicated that none of the samples appeared to be cytotoxic. In addition, estrogen activity of water samples was also investigated by in vitro culturing of water samples with frog, Xenopus laevis, liver slices. Results indicated that none of the water samples from the three rivers studied indicated estrogenic activity. Although cytotoxicity and estrogen activity results were negative, the production of Vtg in male fish suggests further research regarding the presence of estrogenic substances in these rivers. / AFRIKAANSE OPSOMMING: In die huidige studie is die 'Fish Assemblage Integrity Index' (FAIl) toegepas op drie rivere in die suidwes Kaap. Hierdie indeks gebruik visse as bioindikatore van biologies akwatiese integriteit en is gebasseer op die inheemse visspesies wat verwag word in biologiese vishabitatte. Integriteitsklasse is bepaal vir elke studieterrein in die drie rivere wat ondersoek is. 'n Klas C is bepaal vir studieterreine 2 en 4 in die Lourensrivier. Klas F en Klas D is bepaal vir studieterreine 1 en 3 in die rivier onderskeidelik. 'n Klas F is bepaal vir studieterreine 1, 2 en 4 en Klas E en Klas D bepaal vir studieterreine 3 en 5 in die Palmietrivier onderskeidelik. 'n Klas F is bepaal vir studieterrein 1 in die Houtbaairivier waar 'n Klas A bepaal is vir studieterreine 2 en 3. Dit word egter voorgestel dat die FAII aangepas moet word om die algemene lae spesierykheid wat ervaar word in die suidwes Kaap te akkomodeer. Die reaksie van visse, afkomstig van die Lourensrivier, op die langtermyn blootstelling aan estrogeniese stowwe is ook bestudeer. Spesifieke reaksies van endokriene versteuring, soos vitellogeen (Vtg) produksie in manlike visse is ondersoek deur middel van SDS-PAGE gel elektroforese. Resultate toon dat in 60% van die manlike visse Vtg in die plasma teenwoordig was. 'n Ondersoek na abnormale gonade morfologie in manlike en vroulike visse van die Lourensrivier is deur standard histologiese prosedures gedoen. Resultate hiervan dui op geen sigbare abnormaliteite in die gonades nie. Die onmiddelike teenwoordigheid van endokriene versteurders is bestudeer deur die sitotoksisiteit van watermonsters afkomstig van die Lourens, Palmiet en Houtbaai riviere te bepaal. Resultate dui aan dat geen monsters sitotoksies was nie. Die estrogeenaktiwiteit van die watermonsters is ook ondersoek deur van in vitro kulture van watermonsters saam met padda, Xenopus leavis, lewersnitte gebruik te maak. Geen estrogeniese aktiwiteit is in die watermonsters gevind nie. Al is die sitotoksisiteit en estrogeenaktiwiteit resultate negatief, dui die produksie van Vtg in manlike visse op die noodsaaklikheid van verdere navorsing ten opsigte van die teenwoordigheid van estrogeniese stowwe in drie riviere.

M.Sc. (Zoology) / Concern has been shown by Ezemvelo KZN Wildlife for the state of the Lower Pongolo River and Floodplain due to the ever increasing urban and industrial development in the area. This increase in development poses a threat to the fish populations within the system and their ability to support the surrounding communities. Continued spraying of pesticides e.g. DDT within the area are adding to stresses placed on the fish populations through decreasing water quality. The effectiveness of environmental water flows suggested by White et al. (1984), have never been assessed by conservation authorities. This means that the authorities have been unable to fulfil their legislative obligations to establish management and conservation plans (Hughes et al., 2001). Work on the biological responses of fish to anthropogenic stressors such as organic pollutants and metals; have been conducted by a group of experts in the field of ecological health. This section of the project focusses on two fish species, Hydrocynus vittatus and Synodontis zambezensis, sampled within the study area and used for biomarkers and tissue chemical level determination. Standard techniques were applied for Organochlorine Pesticide (OCP) analysis according to Yohannes et al. (2013) and these results have been compared to biomarker responses which have also been completed following standard protocols. Results found within both fish species, show the relationship between accumulation of toxicants and biomarker responses. The highest levels of ΣOCPs where found within H. vittatus at (Mean ± SE) 1101.61 ± 610.97 ng/g lipid weight, the highest constituent being technical grade Dichlorodiphenyltrichloroethane (DDT). High levels of ΣDDT were also found in S. zambezensis, but were lower than levels in H. vittatus and these levels correspond with positioning of each species within the food web. H. vittatus occupies a much higher level in the food web as they are top predators and S. zambezensis falls lower down on the food web due to their reliance on detritus and only small invertebrate species for food. Biomarker responses are clearly affected by bioaccumulation levels and this is especially evident within H. vittatus as Achetylcholinesterase (AChE) is greatly inhibited by high levels of ΣDDT. Changes in the level of biomarker responses are not as apparent in S. zambezensis and this is believed to be food web linked, due to differences in bioconcentration. Data collected during this study will contribute to baseline data on S. zambezensis and add to already accumulated data on H. vittatus. This new data will also help with the improvement of or alteration to already present monitoring programmes in the study area.

M.Sc. (Aquatic Health) / This study took place in the Groot Marico River catchment situated in the North West Province of South Africa. The catchment holds many rivers and associated tributaries that are home to a variety fish species with some being endemic to the catchment. A small minnow species, the Marico barb Barbus motebensis was chosen as the study species due to it being endemic to the catchment with limited information available on them with regards to their reproductive status in terms of their reproductive timing and number of potential offspring. Two tributaries from the upper Groot Marico River were chosen as sampling sites for the collection of B. motebensis across the four seasons. These two tributaries presented two genetically different populations, which allowed for a comparison of the findings to ascertain whether any differences in reproductive aspects occurred between the two tributary populations during the different seasons. The Marico barb is under threat from predation pressure posed by the largemouth bass Micropterus salmoides introduced into the system. Micropterus salmoides is a known alien invasive fish that eats any animal food item it encounters, especially fish. Due to B. motebensis and M. salmoides sharing the same habitat preferences, B. motebensis stands little chance of survival in the same water inhabited by the predatory M. salmoides. Many studies have shown bass to drive other fish species to the point of extirpation from a system through predation pressure. Currently B. motebensis is classified as vulnerable on the Red List of Threatened Species, although this status may be further compromised with the presence of M. salmoides...

Abstract not available