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The lack of a chronological framework for 16th and 17th century northeastern North America has impeded local and regional cultural reconstructions. Based upon the changing style of 16th and early 17th century European glass beads and the settlement patterning of the Neutral Iroquoians of southern Ontario, a chronology has been created. It provides the means to investigate native and European cultural trends during that era, and within this dissertation three topics are examined--the development of the commercial fur trade and its archaeological manifestations, an archaeological definition of the Neutral Iroquoian confederacy, and changes in European material culture recovered from pre-ca. AD 1650 archaeological contexts throughout the Northeast.