The syndrome known as Gastric Dilation Air Sacculitis (GDAS) has recently been described by Lumsden et al. (2002) for Chinook salmon (Oncorhynchus tshawytscha, Walbaum), in seawater (SW) culture in New Zealand. The syndrome is characterised by distended abdomens, gastric dilation and air sacculitis, increased feed conversion ratios (FCR) and mortality. Consequently, financial returns on affected stocks are greatly reduced. A study into the epidemiology and physiology of the syndrome was initiated, working with the major aquaculture company, The New Zealand King Salmon Company (NZKS). The study revealed causative factors of GDAS. GDAS was experimentally induced only in saltwater by feeding a commercially manufactured low-cohesion pelleted diet. Control groups were fed a different diet with high physical cohesion. Low-cohesion pellets have previously been associated with a high incidence of GDAS in commercial sea cages. These data implicated osmoregulatory stress and physical properties of the feed in GDAS development. In addition, gastrointestinal (GI) physiology in GDAS -affected and -control fish was characterised. The process of GDAS development in O. tshawytscha is characterised by a loss of smooth muscle tone of the stomach as it distends. Laplace's law (P= 2T/r, where P is the distending pressure, T is the tension in the wall and r is the radius of the cylinder) predicts that unless muscle mass increases, the ability of the stomach wall to contract will be lost and consequently a loss of GI motor function will result. Therefore, GI circular smooth muscle integrity in terms of (1) stimulated and maximal contractility, (2) osmoregulatory ability of the intestine and the (3) control of the GI system was studied in pathologically affected (+ve) and unaffected (-ve) smolt. Affected fish showed changes in GI circular smooth muscle function and osmoregulatory dysfunction. Feeding different diets induced distinct gastric evacuation patterns. The intestinal brake hypothesis is presented and argued to be the probable mechanism for GDAS development. GDAS (+ve) serum showed the presence of factors capable of contracting gut smooth muscle. In addition, potential humoral mediators of the intestinal brake in fish were investigated.

Vertical mixing is an important process that relates surface fluxes to concentrations of pollutants and other chemical species in the atmosphere. Errors in vertical mixing have been identified as a major source of uncertainties in various atmospheric modeling efforts including tracer transport, weather forecasting, and regional climate simulation. This thesis aims to quantify uncertainties in model-derived mixed layer heights (zi) over North America through direct comparisons between radiosonde observations and four models at different months of the year 2004 through the bulk Richardson number method. Results of this study suggest that considerable errors in zi exist throughout the region with the spatial and temporal variations of the errors differ significantly among the selected models. Over all, errors in zi were larger in global models than in the limited area mesoscale models, and the magnitude of the random error was two times larger than the bias. Notably, spatial regions of with extremely large positive biases correspond to those with especially large random errors. The biases and random errors, however, were not correlated linearly nor can be easily used to predict each other. Uncertainties in model-derived zi were attributed, through errors in the bulk Richardson number, to temperature and horizontal winds. Errors in both horizontal winds and temperatures were found contributing more or less the same to uncertainties in zi, with relative errors in both variables being the greatest in the lowest part of the troposphere. Lastly, independent observations from the cooperative profiler network suggest that data assimilation did not add qualitative advantages for the comparisons presented in this study. The mixed layer height uncertainties demonstrated in this study may provide a guide for selecting a model to simulate regional scale atmospheric transport and for interpreting flux estimation and inversions studies.

Vertical mixing is an important process that relates surface fluxes to concentrations of pollutants and other chemical species in the atmosphere. Errors in vertical mixing have been identified as a major source of uncertainties in various atmospheric modeling efforts including tracer transport, weather forecasting, and regional climate simulation. This thesis aims to quantify uncertainties in model-derived mixed layer heights (zi) over North America through direct comparisons between radiosonde observations and four models at different months of the year 2004 through the bulk Richardson number method. Results of this study suggest that considerable errors in zi exist throughout the region with the spatial and temporal variations of the errors differ significantly among the selected models. Over all, errors in zi were larger in global models than in the limited area mesoscale models, and the magnitude of the random error was two times larger than the bias. Notably, spatial regions of with extremely large positive biases correspond to those with especially large random errors. The biases and random errors, however, were not correlated linearly nor can be easily used to predict each other. Uncertainties in model-derived zi were attributed, through errors in the bulk Richardson number, to temperature and horizontal winds. Errors in both horizontal winds and temperatures were found contributing more or less the same to uncertainties in zi, with relative errors in both variables being the greatest in the lowest part of the troposphere. Lastly, independent observations from the cooperative profiler network suggest that data assimilation did not add qualitative advantages for the comparisons presented in this study. The mixed layer height uncertainties demonstrated in this study may provide a guide for selecting a model to simulate regional scale atmospheric transport and for interpreting flux estimation and inversions studies.

The syndrome known as Gastric Dilation Air Sacculitis (GDAS) has recently been described by Lumsden et al. (2002) for Chinook salmon (Oncorhynchus tshawytscha, Walbaum), in seawater (SW) culture in New Zealand. The syndrome is characterised by distended abdomens, gastric dilation and air sacculitis, increased feed conversion ratios (FCR) and mortality. Consequently, financial returns on affected stocks are greatly reduced. A study into the epidemiology and physiology of the syndrome was initiated, working with the major aquaculture company, The New Zealand King Salmon Company (NZKS). The study revealed causative factors of GDAS. GDAS was experimentally induced only in saltwater by feeding a commercially manufactured low-cohesion pelleted diet. Control groups were fed a different diet with high physical cohesion. Low-cohesion pellets have previously been associated with a high incidence of GDAS in commercial sea cages. These data implicated osmoregulatory stress and physical properties of the feed in GDAS development. In addition, gastrointestinal (GI) physiology in GDAS -affected and -control fish was characterised. The process of GDAS development in O. tshawytscha is characterised by a loss of smooth muscle tone of the stomach as it distends. Laplace's law (P= 2T/r, where P is the distending pressure, T is the tension in the wall and r is the radius of the cylinder) predicts that unless muscle mass increases, the ability of the stomach wall to contract will be lost and consequently a loss of GI motor function will result. Therefore, GI circular smooth muscle integrity in terms of (1) stimulated and maximal contractility, (2) osmoregulatory ability of the intestine and the (3) control of the GI system was studied in pathologically affected (+ve) and unaffected (-ve) smolt. Affected fish showed changes in GI circular smooth muscle function and osmoregulatory dysfunction. Feeding different diets induced distinct gastric evacuation patterns. The intestinal brake hypothesis is presented and argued to be the probable mechanism for GDAS development. GDAS (+ve) serum showed the presence of factors capable of contracting gut smooth muscle. In addition, potential humoral mediators of the intestinal brake in fish were investigated.