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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.

Electrophoretic behaviour of polystyrene microspheres in agarose gels

To, Kwong-yuk. 1993 (has links)
Thesis (Ph. D.)--University of Hong Kong, 1993.

The development of a high-resolution, analytical and preparative gel electrophoresis system and its application in determining the binding constants of biologically-active, intercalating fluorescent stains

Hassur, Steven M. 1976 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1976. Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references

Temperature gradient gel electrophoresis development and application

Hosseini, Seyed Homayoun 1994 (has links)
No description available.

Electrophoretic behaviour of polystyrene microspheres in agarose gels

杜光旭, To, Kwong-yuk. 1993
published_or_final_version Biochemistry Doctoral Doctor of Philosophy

DNA electrophoresis in agarose gels a new mobility vs. DNA length dependence

Beheshti, Afshin. Van Winkle, David H. 2002 (has links)
Thesis (Ph. D.)--Florida State University, 2002. Advisor: Dr. David H. Van Winkle, Florida State University, College of Arts and Sciences, Dept. of Physics. Title and description from dissertation home page (viewed Oct. 3, 2003). Includes bibliographical references.

Studies on agar gel electrophoresis techniques, applications.

Wieme, R. J. 1959 (has links)
Proefschrift--Ghent. Includes bibliographical references.

Prospective analysis of real time minimal residual disease assessment in childhood acute lymphoblastic leukaemia prior to bone marrow transplantation

Moppett, John Paul 2003 (has links)
No description available.

The isolation and characterisation of mutants of Amaranthus edulis lacking key enzymes of the C4 pathway

Dever, Louisa Violet 1995 (has links)
No description available.

Studies on agar gel electrophoresis: techniques, applications.

Wieme, R. J. 1959 (has links)
Proefschrift--Ghent. Includes bibliography.

An Investigation on Gel Electrophoresis with Quantum Dots End-labeled DNA

Chen, Xiaojia 2008 (has links)
Invented in the 1950s, gel electrophoresis has now become a routine analytical method to verify the size of nucleic acids and proteins in molecular biology labs. Conventional gel electrophoresis can successfully separate DNA fragments from several base pairs to a few tens of kilo base pairs, beyond which a point is reached that DNA molecules cannot be resolved due to the size independent mobility. In this case, pulsed field gel electrophoresis (PFGE) was introduced to extend the range of DNA fragment sizes that can be effectively separated. But despite the incredible success of PFGE techniques, some important drawbacks remain. First, separation time is extremely long, ranging from several hours to a few days. Second, detection methods still rely on staining the gel after the run. Real time observation and study of band migration behavior is impossible due to the large size of the PFGE device. Finally, many commercial PFGE instruments are relatively expensive, a factor that can limit their accessibility both for routine analytical and preparative use as well as for performing fundamental studies. In this research, a miniaturized PFGE device was constructed with dimension 2cm x 2.6cm, capable of separating DNA fragments ranging from 2.5kb to 32kb within three hours using low voltage. The separation process can be observed in real time under a fluorescence microscope mounted with a cooled CCD camera. Resolution and mobility of the sample were measured to test the efficiency of the device. We also explored manipulating DNA fragments by end labeling DNA molecules with quantum dot nanocrystals. The quantum dot-DNA conjugates can be further modified through binding interactions with biotinylated single-stranded DNA primers. Single molecule visualization was performed during gel electrophoresis and the extension length, entanglement probability and reorientation time of different conjugates were measured to study their effect on DNA migration through the gel. Finally, electrophoresis of DNA conjugates was performed in the miniaturized PFGE device, and shaper bands were observed compared with the non end-labeled sample. Furthermore, by end-labeling DNA with quantum dots, the migration distance of shorter fragments is reduced, providing the possibility of separating a wider range of DNA fragment sizes on the same gel to achieve further device miniaturization.

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