Previous research confirms that gender affects organizations and the overall organizational culture. Organizations are heavily gendered and, in most cases, to the disadvantage of women. It has been shown that gender bias within male-dominated organizations creates the queen bee syndrome, characterized by a lack of solidarity and hostile behavior amongst women. Because gender bias exists in all organizations, it is reasonable to believe it also exists in female-dominated organizations. This study examines the queen bee syndrome in female-dominated organizations, analyzing how the characteristics permeate the organizational culture. The study employs a qualitative research method and collects data through qualitative surveys with LiVO members, a Swedish union organization for managers within the health and care sector. The study aims to build on theory regarding the queen bee syndrome and extend knowledge about gendered organizations and female-dominated organizations. This study suggests that signs of the queen bee syndrome, to some extent, permeate the organizational culture in female-dominated organizations. However, the result also reveals that complexity and duality exist. The triggering structures that create the queen bee syndrome need to be addressed rather than the gender composition to counteract queen bee behavior.