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Identification of a Minimal Cis-element and Cognate Trans-factors Required for the Regulation of Rac2 Gene Expression during K562 Cell DifferentiationMuthukrishnan, Rajarajeswari 18 March 2009 (has links)
Indiana University-Purdue University, Indianapolis / This dissertation examines the molecular mechanisms regulating Rac2 gene expression during cell differentiation and identification of a minimal cis-element required for the induction of Rac2 gene expression during K562 cell differentiation. The Rho family GTPase Rac2 is expressed in hematopoietic cell lineages and is further up-regulated upon terminal myeloid cell differentiation. Rac2 plays an important role in many hematopoietic cellular functions, such as neutrophil chemotaxis, superoxide production, cytoskeletal reorganization, and stem cell adhesion. Despite the crucial role of Rac2 in blood cell function, little is known about the mechanisms of Rac2 gene regulation during blood cell differentiation. Previous studies from the Skalnik lab determined that a human Rac2 gene fragment containing the 1.6 kb upstream and 8 kb downstream sequence directs
lineage-specific expression of Rac2 in transgenic mice. In addition, epigenetic modifications such as DNA methylation also play important roles in the lineage-specific expression of Rac2.
The current study investigated the molecular mechanisms regulating human Rac2 gene expression during cell differentiation using chemically induced megakaryocytic differentiation of the human chronic myelogenous leukemia cell line K562 as the model system. Phorbol 12-myristate 13-acetate (PMA) stimulation of K562 cells resulted in increased Rac2 mRNA expression as analyzed by real time-polymerase chain reaction (RT-PCR). Luciferase reporter gene assays revealed that increased transcriptional activity of the Rac2 gene is mediated by the Rac2 promoter region. Nested 5’- deletions of the promoter region identified a critical regulatory region between -4223 bp and -4008 bp upstream of the transcription start site. Super shift and chromatin immunoprecipitation assays indicated binding by the transcription factor AP1 to three distinct binding sites within the 135 bp minimal regulatory region. PMA stimulation of K562 cells led to extensive changes in chromatin structure, including increased histone H3 acetylation, within the 135 bp Rac2 cis-element.
These findings provide evidence for the interplay between epigenetic modifications, transcription factors and cis-acting regulatory elements within the Rac2 gene promoter region to regulate Rac2 expression during K562 cell differentiation.
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Sex- and tissue-specific expression of different members of the mouse major urinary protein multigene familyMcIntosh, Iain January 1988 (has links)
No description available.
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Development of a strategy for genetic transformation of plant mitochondriaMoore, Ian Robert January 1989 (has links)
No description available.
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Gene structure and expression of human pro-alpha2(XI) collagen (col11A2) gene呂志恆, Lui, Chi-hang, Vincent. January 1995 (has links)
published_or_final_version / Biochemistry / Doctoral / Doctor of Philosophy
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Gene expression study in ovary cancer黃虹麗, Wong, Hung-lai. January 2008 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
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Characterization of Sad1/UNC-84 domain protein 2 (SUN2)Liang, Ying, 梁瑛 January 2006 (has links)
published_or_final_version / abstract / Paediatrics and Adolescent Medicine / Doctoral / Doctor of Philosophy
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Transcriptional regulation of the human secretin receptor gene expression何寶琪, Ho, Po-ki. January 1999 (has links)
published_or_final_version / Zoology / Master / Master of Philosophy
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Algorithms for the analysis of gene expression dataVenet, David 07 December 2004 (has links)
High-throughput gene expression data have been generated on a large scale by biologists.
The thesis describe a set of tools for the analysis of such data. It is specially gearded towards microarray data.
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Protein-DNA interactions of transcription factors reponsible for cell-type specificity in Saccharomyces cerevisiaeTan, Song January 1989 (has links)
No description available.
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Transcriptionally competent and repressed chromatinVerreault, Alain January 1993 (has links)
No description available.
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