Genetic variants of milk proteins are the result of mutation causing substitutions or deletions of amino acids in the peptides. Certain variants are closely associated with milk production, milk composition and milk qualities such as heat stability and cheesemaking properties. All of the milk protein variants known so far, have been detected by electrophoresis because mutations which have occurred gave rise to changes in net charges. "Silent" variants involve amino acid substitutions which are not accompanied by charge differences and hence would not be identified by electrophoresis. The aim of this project was to search for silent variants of $ alpha sb{ rm s1}$-casein, $ beta$-casein and $ kappa$-casein by application of reversed-phase HPLC for the identification of mutants causing changes in hydrophobicities of peptides. / Whole casein from 281 Holstein and Ayrshire milk samples were fractionated by DEAE-cellulose ion-exchange chromatography, using an FPLC system. The pure forms of major caseins ($ alpha sb{ rm s1}$-casein, $ beta$-casein and $ kappa$-casein) were hydrolysed by trypsin and the digests obtained therefrom were resolved by reversed-phase HPLC. Comparison of peptide profiles within the same electrophoretic variant of homozygous caseins indicated that 24 out of 264 $ alpha sb{ rm s1}$-casein BB, 9 out of 57 $ beta$-casein A$ sp1$A$ sp1$, 5 out of 55 $ beta$-casein A$ sp2$A$ sp2$ and 8 out of 188 $ kappa$-casein AA could be classified as potential silent variants.

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Amendements: leaves 252-254. / Copies of author's previously published articles inserted. / Bibliography: leaves 226-251. / vi, 254 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / The CYP2C19 and CYP2D6 genetic polymorphisms control the oxidative metabolism of many different drug classes. Populations are separated into groups of extensive metabolisers (EM), poor metabolisers (PM), and in the case of CYP2D6, ultra-rapid metabolisers (UM). In vitro studies using human liver microsomes were conducted to examine the kinetics of the oxidative metabolism of flunitrazepam, and which CYP450 enzymes mediate the oxidative metabolism of flunitrazepam, (S)-mephenytoin and proguanil. / Thesis (Ph.D.)--University of Adelaide, Dept. of Clinical and Experimental Pharmacology, 2000

Thesis (M. Res. (Med.))--University of Hong Kong, 2007.

Thesis (M.S. in Interdisciplinary Studies: Neurogenetics)--Vanderbilt University, Dec. 2007. / Title from title screen. Includes bibliographical references.

Thesis (Ph. D. in Psychology)--Vanderbilt University, Dec. 2007. / Title from title screen. Includes bibliographical references.

Thesis (MSc)--University of Stellenbosch, 2007. / Bibliography. Also available via the Internet.

Thesis (Ph.D.) -- University of Adelaide, Dept. of Clinical and Experimental Pharmacology, 2000. / Amendements: leaves 252-254. Copies of author's previously published articles inserted. Bibliography: leaves 226-251.

Thesis (Ph. D.)--University of Hong Kong, 2008. / Includes bibliographical references (p. 125-145) Also available in print.

Thesis (Ph. D.)--University of Hong Kong, 2008. / Includes bibliographical references (leaf 194-218) Also available in print.