by Wang Wei. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1991. / Includes bibliographical references (leaves 154-168). / ACKNOWLEDGMENTS --- p.v / SUMMARY --- p.vi / PUBLICATIONS --- p.viii / STATEMENT OF ORIGINALITY --- p.ix / LIST OF ABBREVIATIONS --- p.x / Chapter CHAPTER 1 --- INTRODUCTION --- p.1 / Chapter CHAPTER 2 --- LITERATURE REVIEW --- p.5 / Chapter 2.1 --- Chromosome --- p.6 / Chapter 2.2 --- Chromosome and Human Disease --- p.9 / Chapter 2.3 --- Chromosome and Tumour --- p.12 / Chapter 2.4 --- Chromosome in Gynaecologic Tumours --- p.17 / Chapter 2.4.1 --- Cervical tumour --- p.17 / Chapter 2.4.2 --- Uterine corpus tumour --- p.20 / Chapter 2.4.3 --- Ovarian tumour --- p.23 / Chapter 2.5 --- Methodology in cytogenetics --- p.26 / Chapter 2.5.1 --- Materials --- p.27 / Chapter 2.5.2 --- Methods of chromosome preparation --- p.28 / Chapter 2.5.3 --- Karyotype analysis --- p.34 / Chapter 2.6 --- Problems of cytogenetic analysis in solid tumour --- p.42 / Chapter CHAPTER 3 --- MATERIALS AND METHODS --- p.47 / Chapter 3.1 --- Chemicals and Solutions --- p.48 / Chapter 3.2 --- Chromosome preparation from solid gynaecologic tumours --- p.50 / Chapter 3.2.1 --- Solid tumour specimens --- p.50 / Chapter 3.2.2 --- Chromosome preparation --- p.54 / Chapter 3.3 --- Chromosome preparation from an established ovarian carcinoma cell line --- p.61 / Chapter 3.3.1 --- Origin of OCC1 cell line --- p.61 / Chapter 3.3.2 --- Characteristics of OCC1 cell line --- p.61 / Chapter 3.3.3 --- Maintaining of OCC1 cell line --- p.62 / Chapter 3.3.4 --- Chromosome preparation --- p.62 / Chapter 3.4 --- Karyotype analysis --- p.65 / Chapter CHAPTER 4 --- RESULTS --- p.66 / Chapter 4.1 --- Cytogenetic features of gynaecologic solid tumour --- p.67 / Chapter 4.1.1 --- Cervical cancer --- p.67 / Chapter 4.1.2 --- Uterine corpus cancer --- p.94 / Chapter 4.1.3 --- Ovarian cancer --- p.104 / Chapter 4.2 --- Cytogenetic features of OCC1 ovarian carcinoma cell line --- p.114 / Chapter CHAPTER 5 --- DISCUSSION --- p.123 / Chapter 5.1 --- Methodology of chromosome preparation in solid tumour --- p.124 / Chapter 5.2 --- Chromosome changes in gynaecologic solid tumour --- p.126 / Chapter 5.2.1 --- Cervical cancer --- p.126 / Chapter 5.2.2 --- Uterine corpus cancer --- p.132 / Chapter 5.2.3 --- Ovarian cancer --- p.138 / Chapter 5.2.4 --- In summary --- p.141 / Chapter 5.3 --- Chromosome changes in an OCC1 ovarian carcinoma cell line --- p.143 / Chapter CHAPTER 6 --- CONCLUSION --- p.148 / REFERENCES --- p.154