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Au, Chun Hang. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 124-146). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract also in Chinese.

Hui Cheung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 166-172). / Abstracts in English and Chinese. / Abstract --- p.iii / Acknowledgments --- p.vi / Abbreviations --- p.vii / Table of Contents --- p.viii / Table of Figures --- p.xiii / Table of Tables --- p.xviii / Chapter Chapter 1. --- Literature Reviews / Chapter 1.1 --- Functional Genomics and Its Developments --- p.1 / Chapter 1.1.1 --- Introduction --- p.1 / Chapter 1.1.2 --- "Transcriptomics, Proteomics and Metabolomics" --- p.1 / Chapter 1.1.3 --- Gene-perturbing Strategies --- p.3 / Chapter 1.1.4 --- Applications of Functional Genomics --- p.4 / Chapter 1.2 --- Serial Analysis of Gene Expression (SAGE) and Generation of Longer cDNA Fragments from SAGE tags for Gene Identification (GLGI) --- p.6 / Chapter 1.2.1 --- Introduction --- p.6 / Chapter 1.2.2 --- Principles and Methods of SAGE --- p.6 / Chapter 1.2.3 --- Data Analysis --- Bioinformatics --- p.9 / Chapter 1.2.4 --- Applications of SAGE --- p.9 / Chapter 1.2.5 --- Modifications of SAGE --- p.10 / Chapter 1.2.6 --- Principles and Methods of GLGI --- p.11 / Chapter 1.2.7 --- Applications and Improvements of GLGI --- p.14 / Chapter 1.3 --- Transformation --- p.15 / Chapter 1.3.1 --- Introduction --- p.15 / Chapter 1.3.2 --- Different Methods of Transformation --- p.15 / Chapter 1.3.2.1 --- General Transformation Strategy --- p.15 / Chapter 1.3.2.2 --- Polyethylene Glycol (PEG)-mediated Transformation --- p.16 / Chapter 1.3.2.3 --- Restriction Enzyme Mediated Integration (REMI) --- p.16 / Chapter 1.3.2.4 --- Electroporation --- p.17 / Chapter 1.3.2.5 --- Particle Bombardment --- p.17 / Chapter 1.3.3 --- The Future Needs of Transformation --- p.18 / Chapter 1.4 --- RNA Silencing --- p.20 / Chapter 1.4.1 --- Introduction --- p.20 / Chapter 1.4.2 --- Major Components and Principles of RNAi --- p.21 / Chapter 1.4.3 --- Applications of RNA Silencing --- p.23 / Chapter 1.5 --- The Target Organism Lentinula edodes --- p.25 / Chapter 1.5.1 --- Introduction --- p.25 / Chapter 1.5.2 --- The Life Cycle of L. edodes --- p.26 / Chapter 1.5.3 --- Biochemical and Molecular Studies on L. edodes --- p.27 / Chapter 1.5.4 --- Prospectus --- p.29 / Chapter Chapter 2. --- Development of Methods for Studying Gene Function in Lentinula edodes / Chapter 2.1 --- Introduction --- p.30 / Chapter 2.2 --- Materials and Methods --- p.32 / Chapter 2.2.1 --- Cultivation of Lentinula edodes --- p.32 / Chapter 2.2.2 --- Proplast Release and Regeneration --- p.32 / Chapter 2.2.3 --- Preparation of Plasmid DNA --- p.33 / Chapter 2.2.4 --- Selectable Marker …Bialaphos --- p.35 / Chapter 2.2.5 --- Transformation --- p.35 / Chapter 2.2.5.1 --- Electroporation --- p.35 / Chapter 2.2.5.2 --- PEG-mediated Transformation --- p.36 / Chapter 2.3 --- Results --- p.37 / Chapter 2.3.1 --- Cultivation of Lentinula edodes --- p.37 / Chapter 2.3.2 --- Proplast Release and Regeneration --- p.37 / Chapter 2.3.3 --- Preparation of Plasmid DNA --- p.43 / Chapter 2.3.4 --- Selectable Marker--- Bialaphos --- p.43 / Chapter 2.3.5 --- Transformation --- p.46 / Chapter 2.3.5.1 --- Electroporation --- p.46 / Chapter 2.3.5.2 --- PEG-mediated Transformation --- p.46 / Chapter 2.4 --- Discussions and Conclusions --- p.57 / Chapter Chapter 3. --- Identification of Interested Genes in Expression Profile of SAGE using GLGI Method. / Chapter 3.1 --- Introduction --- p.61 / Chapter 3.1.1 --- Results of SAGE Analysis --- p.61 / Chapter 3.1.2 --- Use of GLGI Method for Extension of SAGE Tags --- p.63 / Chapter 3.1.3 --- 5´ة Extension of GLGI (5'GLGI) --- p.65 / Chapter 3.1.3.1 --- Introduction --- p.65 / Chapter 3.1.3.2 --- "Overall strategy of 5, GLGI Method" --- p.67 / Chapter 3.1.3.3 --- Two-Steps PCR Method --- p.69 / Chapter 3.2 --- Generation of Longer cDNA Fragments from SAGE tags for Gene Identification (GLGI) --- p.71 / Chapter 3.2.1 --- Materials and Methods (GLGI Analysis) --- p.71 / Chapter 3.2.1.1 --- Total RNA Extraction --- p.71 / Chapter 3.2.1.2 --- Messenger RNA (mRNA) Extraction --- p.72 / Chapter 3.2.1.3 --- Preparation of 3´ة cDNA for GLGI --- p.73 / Chapter 3.2.1.4 --- NIaIII digestion of double strand cDNA --- p.74 / Chapter 3.2.1.5 --- PCR amplification of the 3'-cDNAs (Optional) --- p.77 / Chapter 3.2.1.6 --- GLGI Amplification of The Target Template --- p.80 / Chapter 3.2.1.7 --- DNA Cloning (Optional) --- p.82 / Chapter 3.2.1.8 --- Sequencing of GLGI PCR products --- p.85 / Chapter 3.2.2 --- 5' Materials and Methods (5' GLGI Analysis) --- p.86 / Chapter 3.2.2.1 --- Preparation of unique antisense primers --- p.86 / Chapter 3.2.2.2 --- 5' extension of GLGI products --- p.87 / Chapter 3.2.2.3 --- DNA Cloning (Optional) --- p.89 / Chapter 3.2.2.4 --- Sequencing of 5' GLGI PCR products --- p.89 / Chapter 3.2.3 --- Results (GLGI Analysis) --- p.90 / Chapter 3.2.3.1 --- Total RNA Extraction --- p.90 / Chapter 3.2.3.2 --- Messenger RNA Extraction --- p.90 / Chapter 3.2.3.3 --- Preparation of 3' cDNA for GLGI --- p.90 / Chapter 3.2.3.4 --- NIaIII digestion of double strand cDNA --- p.94 / Chapter 3.2.3.5 --- GLGI Amplification of The Target Template --- p.94 / Chapter 3.2.3.6 --- Sequencing of GLGI PCR products --- p.103 / Chapter 3.2.4 --- Results (5' GLGI Analysis) --- p.111 / Chapter 3.2.4.1 --- 5' extension of GLGI products --- p.111 / Chapter 3.2.4.2 --- Sequencing of 5´ة GLGI PCR products --- p.116 / Chapter 3.3 --- Discussions and Conclusions --- p.126 / Chapter 3.3.1 --- GLGI amplification of the target template --- p.126 / Chapter 3.3.2 --- 5' extension of GLGI products --- p.129 / Chapter 3.3.3 --- Two-Steps PCR Method --- p.130 / Chapter 3.3.4 --- Sequencing results of GLGI method and 5' GLGI method --- p.131 / Chapter Chapter 4. --- Identification of Unknown EST Using PCR Method With cDNA Library / Chapter 4.1 --- Introduction --- p.134 / Chapter 4.2 --- Materials and Methods --- p.134 / Chapter 4.2.1 --- Extension of 5' end of EST sequence by PCR method --- p.134 / Chapter 4.2.2 --- Purification of PCR products --- p.136 / Chapter 4.2.3 --- Sequencing of Extended EST products --- p.136 / Chapter 4.3 --- Results --- p.137 / Chapter 4.3.1 --- Extension of 5' end of EST sequence by PCR method --- p.137 / Chapter 4.3.2 --- Sequencing of Extended EST products --- p.137 / Chapter 4.4 --- Discussions and Conclusions --- p.147 / Chapter Chapter 5. --- General Discussions --- p.151 / Appendix I --- p.156 / Reference --- p.166

Graduation date: 2004

Marker information in the new oilseed crop, meadowfoam, is limited. Molecular markers to facilitate meadowfoam breeding and cultivar improvement are not available. The knowledge of genetic relationships among recent germplasm is not known. The objectives of this study were (i) to gain an understanding of genetic diversity and relationship patterns among germplasm. (ii) to construct a genetic linkage map, and (iii) to map genes and QTLs (Quantitative Trait Loci) underlying erucic and dienoic acid concentrations in seed oils. We fingerprinted meadowfoam 41 accessions of section inflexae of family Limnanthaceae using 176 AFLP markers. Polymorphic information content (PIC) scores were high in 42.6% of the markers and ranged from 0.45 to 0.5. Genetic distance estimates ranged from 0.14 to 0.55 with an average of 0.44. The clustering phenogram showed concordance with taxonomic classification. The first three principal component analyses accounted for 37% of the total variation of genetic distance estimated. We concluded that the genetic diversity of elite and exotic germplasm in section Inflexae was high. The AFLP genetic linkage map for meadowfoam was built using inter-subspecific backcross progeny between OMF40-11 (Limnanthes. alba spp. alba) and 0MF64 (L. alba spp. versicolor). The map was comprised of 104 loci in five linkage groups, with 14 to 28 loci per linkage group. The map covered 698.3 cM with a mean density of 6.7 cM. The lengths of the linkage groups varied from 110.3 to 168.0 cM. AFLP loci were randomly distributed throughout the genome with no centromeric clustering. Genetic maps of meadowfoam can be rapidly Constructed using a small number of AFLP primer combinations. We utilized the AFLP genetic linkage map to map genes and QTLs underlying erucic and dienoic acid concentrations in seed oils. The QTL analyses were performed using interval mapping. QTL affecting erucic and dienoic acids was mapped to linkage group four at the E locus, which controlled seed oil phenotypic differences between the two subspecies, alba and versicolor. The effect of E locus was pleiotropic. QTLs with significant effects on content of erucic and dienoic acid other than the effects of E locus were not found in this backcross population. / Graduation date: 2000

Making quality and winterhardiness in barley are "ultimate" phenotypes composed of component, quantitatively inherited traits. A 69-point genome map of the seven chromosomes of barley was used, in conjunction with multi-environment phenotypes for grain yield and malting quality, to determine the chromosome locations of quantitative trait loci (QTLs). A combined analysis of the two environments identified QTLs that were both common and unique to each environment. Dispersed QTLs with positive relationships provide ready targets for marker-assisted selection. Overlapping QTLs for agronomic and making quality QTLs with favorable alleles contributed by alternate parents will require further, higher resolution mapping to determine if negative relationships are due to linkage or pleiotropy. There is preliminary evidence for orthologous agronomic trait and malting QTLs in barley. This QTL analysis will hopefully assist in the rapid development of winter making varieties that will maximize the profitability of Oregon barley production. / Graduation date: 1994

published_or_final_version / abstract / toc / Ecology and Biodiversity / Doctoral / Doctor of Philosophy

Thesis (Ph. D.)--University of Hawaii at Manoa, 2004. / Includes bibliographical references (leaves 147-159). / Also available by subscription via World Wide Web / xv, 159 leaves, bound ill. (some col.) 29 cm

Thesis(Master)--İzmir Institute of Technology, İzmir, 2005 / Keywords: Antioxidants, antioxidant activity, tomato, genetic mapping, genetic markers. Includes bibliographical references (p.47-51)