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Magnitude and controls of microbial nitrate production in the streams and till of a glaciated alpine catchment, Canadian Rocky Mountains, AlbertaDoxsey-Whitfield, Erin 26 April 2012 (has links)
In the summer of 2010, fieldwork was conducted in the Robertson Valley, Canadian Rocky Mountains, Alberta to assess the magnitude and controls of microbial nitrification in proglacial till and in supraglacial, subglacial, and proglacial streams. Seasonal precipitation and glacial and proglacial runoff was sampled for hydrochemical and stable isotope analyses (δ18O and δ15N of nitrate [NO3-]). Lower Ca:Mg ratios, higher mean Σmajor ions, and an increased importance of reactions with slower dissolution kinetics in subglacial streams and proglacial seeps indicated waters here experienced longer rock-water contact time than in dilute supraglacial streams. Additionally, waters emanating from longer residence time flowpaths acquired substantial NO3- from nitrification reactions. Using δ18O-NO3- in a simple end-member mixing model, the fraction of NO3- derived from microbial nitrification was estimated to be 44 to 56% in the two subglacial streams, and greater than 80% in proglacial seeps. These results show that atmospherically-derived nitrogen (N) in this glacial valley undergoes substantial biological cycling prior to export in surface runoff. Water flowing from the east subglacial stream (RE) received a larger portion of its melt from a sediment-rich, slow drainage system and had a higher proportion of nitrified NO3- compared to the west subglacial stream (RW), where runoff was similar in composition to supraglacial runoff, indicating that the nature of subglacial flowpaths is an important factor in determining the amount of microbially-cycled nutrients that are exported from a glacier. Sixteen 34-day in situ soil incubations revealed that net mineralization and net nitrification occurred at all four sampling sites in the glacier forefield along a 1.6 km chronosequence; however, there was no significant difference among these rates with time since deglaciation or temperature. Instead, net mineralization and net nitrification rates were significantly correlated (p < 0.05, n = 16) with measured physical and chemical soil variables, including total organic carbon, total N, bulk density, pH, and clay content, suggesting that substrate availability is a larger control on N-cycling processes than time since deglaciation. High variability in inorganic soil N pools and N-cycling rates indicates that there are likely hot spots of biogeochemical activity within glacial till. / Thesis (Master, Geography) -- Queen's University, 2012-04-26 14:47:17.29
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Bacterial communities in glacier forefields of the Larsemann Hills, East Antarctica : structure, development & adaptationBajerski, Felizitas January 2013 (has links)
Antarctic glacier forfields are extreme environments and pioneer sites for ecological succession. The Antarctic continent shows microbial community development as a natural laboratory because of its special environment, geographic isolation and little anthropogenic influence. Increasing temperatures due to global warming lead to enhanced deglaciation processes in cold-affected habitats and new terrain is becoming exposed to soil formation and accessible for microbial colonisation. This study aims to understand the structure and development of glacier forefield bacterial communities, especially how soil parameters impact the microorganisms and how those are adapted to the extreme conditions of the habitat. To this effect, a combination of cultivation experiments, molecular, geophysical and geochemical analysis was applied to examine two glacier forfields of the Larsemann Hills, East Antarctica. Culture-independent molecular tools such as terminal restriction length polymorphism (T-RFLP), clone libraries and quantitative real-time PCR (qPCR) were used to determine bacterial diversity and distribution. Cultivation of yet unknown species was carried out to get insights in the physiology and adaptation of the microorganisms. Adaptation strategies of the microorganisms were studied by determining changes of the cell membrane phospholipid fatty acid (PLFA) inventory of an isolated bacterium in response to temperature and pH fluctuations and by measuring enzyme activity at low temperature in environmental soil samples.
The two studied glacier forefields are extreme habitats characterised by low temperatures, low water availability and small oligotrophic nutrient pools and represent sites of different bacterial succession in relation to soil parameters. The investigated sites showed microbial succession at an early step of soil formation near the ice tongue in comparison to closely located but rather older and more developed soil from the forefield. At the early step the succession is influenced by a deglaciation-dependent areal shift of soil parameters followed by a variable and prevalently depth-related distribution of the soil parameters that is driven by the extreme Antarctic conditions. The dominant taxa in the glacier forefields are Actinobacteria, Acidobacteria, Proteobacteria, Bacteroidetes, Cyanobacteria and Chloroflexi. The connection of soil characteristics with bacterial community structure showed that soil parameter and soil formation along the glacier forefield influence the distribution of certain phyla. In the early step of succession the relative undifferentiated bacterial diversity reflects the undifferentiated soil development and has a high potential to shift according to past and present environmental conditions. With progressing development environmental constraints such as water or carbon limitation have a greater influence.
Adapting the culturing conditions to the cold and oligotrophic environment, the number of culturable heterotrophic bacteria reached up to 108 colony forming units per gram soil and 148 isolates were obtained. Two new psychrotolerant bacteria, Herbaspirillum psychrotolerans PB1T and Chryseobacterium frigidisoli PB4T, were characterised in detail and described as novel species in the family of Oxalobacteraceae and Flavobacteriaceae, respectively. The isolates are able to grow at low temperatures tolerating temperature fluctuations and they are not specialised to a certain substrate, therefore they are well-adapted to the cold and oligotrophic environment.
The adaptation strategies of the microorganisms were analysed in environmental samples and cultures focussing on extracellular enzyme activity at low temperature and PLFA analyses. Extracellular phosphatases (pH 11 and pH 6.5), β-glucosidase, invertase and urease activity were detected in the glacier forefield soils at low temperature (14°C) catalysing the conversion of various compounds providing necessary substrates and may further play a role in the soil formation and total carbon turnover of the habitat. The PLFA analysis of the newly isolated species C. frigidisoli showed that the cold-adapted strain develops different strategies to maintain the cell membrane function under changing environmental conditions by altering the PLFA inventory at different temperatures and pH values. A newly discovered fatty acid, which was not found in any other microorganism so far, significantly increased at decreasing temperature and low pH and thus plays an important role in the adaption of C. frigidisoli.
This work gives insights into the diversity, distribution and adaptation mechanisms of microbial communities in oligotrophic cold-affected soils and shows that Antarctic glacier forefields are suitable model systems to study bacterial colonisation in connection to soil formation. / Gletschervorfelder der Antarktis stellen extreme Habitate dar und sind Pionierstandorte biologischer Sukzession. Insbesondere unter Berücksichtigung zuletzt beobachteter und vorausgesagter Erwärmungstrends in der Antarktis und der Relevanz der Mikroorganismen für das Antarktische Ökosystem, ist es essentiell mehr Informationen über die Entwicklung frisch exponierter Gletschervorfelder zu erlangen. Ziel dieser Studie ist es, die Struktur und Entwicklung bakterieller Gletschervorfeldgemeinschaften zu verstehen, insbesondere wie die Mikroorganismen von den Bodenparametern beeinflusst werden und wie diese sich an die extremen Bedingungen des Habitats anpassen. Für die Untersuchung der Proben von zwei Gletschervorfeldern aus den Larsemann Bergen der Ostantarktis diente eine Kombination aus Kultivierungsexperimenten und molekularen, geophysikalischen und geochemischen Analysen. Die untersuchten Gletschervorfelder sind durch extrem niedrige Temperaturen, einer geringen biologischen Wasserverfügbarkeit und oligotrophe Nährstoffgehalte charakterisiert und zeigen unterschiedliche Entwicklungsstufen in Verbindung zu den Bodenparametern. In einem frühen Schritt der Bodenbildung in der Nähe der Gletscherzunge sind die Gemeinschaften undifferenziert, doch mit fortschreitender Entwicklung nimmt de Einfluss von Wasser- und Nährstofflimitationen zu. Nachdem die Kultivierungsbedingungen den kalten und nährstoffarmen Bedingungen des Habitats angepasst wurden, konnten 108 koloniebildende Einheiten heterotropher Bakterien pro Gramm Boden angereichert und daraus 148 Isolate gewonnen werden. Zwei neue psychrotolerante Bakterien, Herbaspirillum psychrotolerans PB1T und Chryseobacterium frigidisoli PB4T, wurden detailiert charakterisiert und als jeweils neue Spezies beschrieben. Die Anpassungsstrategien der Mikroorganismen an die extremen antarktischen Bedingungen zeigten sich in der Aktivität extrazellulärer Enzyme bei niedriger Temperatur, die mit derer temperierter Habitate vergleichbar ist, und in der Fähigkeit der Mikroorganismen, die Fettsäurezusammensetzung der Zellmembran zu ändern. Eine neue Fettsäure, die bisher in keinen anderen Mikroorganismus gefunden wurde, spielt eine entscheidende Rolle in der Anpassung des neu-beschriebenen Bakteriums C. frigidisoli an niedrige Temperaturen und saure pH-Werte. Diese Arbeit gibt einen Einblick in die Vielfalt, Verteilung und Anpassung mikrobieller Gemeinschaften in nährstoffarmen und Kälte-beeinflussten Habitaten und zeigt, dass Antarktische Gletschervorfelder geeignete Modellsysteme, um bakterielle Besiedelung in Verbindung zu Bodenbildung zu untersuchen.
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Moving at a glacial pace: a biogeomorphological analysis of ecological succession in recently deglaciated terrain in the Selkirk Range, BCLincoln, Astra 02 May 2022 (has links)
This research developed a novel workflow for combining different types and scales of data to understand the development of small, steep, and sheltered glacial forefields across space and time using the Avalanche glacier of the Selkirk Range, BC as a case study. As glaciers recede, symbiotic geomorphological and ecological feedback loops determine the ecological succession in recently deglaciated terrain, which can in turn effect landform stability and water quality downstream. In order to describe emergent land cover patterns in the forefield, this research uses Corenbilt’s (2007) fluvial biogeomorphic succession (FBS) framework to interpret a century of land cover changes. To do so, an experimental protocol was developed that combined remotely sensed data – repeat photographs, historic air photographs, satellite imagery, and digital elevation models – and data collected in-situ using a photo transect method. Analysis of more than a century of photographs determined that the Avalanche glacier is receding at a slower rate than has been observed in the region’s larger glaciers, subsequently leading to a slower rate of forefield habitat expansion. Still, all four stages of fluvial biogeomorphological succession were found across the Avalanche glacier’s forefield. It was found that in the Avalanche forefield, terrain age seems to place a limit on which successional stage is possible at any given location within the forefield, but topographic features like slope angle seemed to influence succession patterns within areas that had the same terrain age. Further research is needed to see whether these findings are consistent for similar steep, small, and sheltered glaciers in the region. / Graduate
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