Thesis (M.S.)--University of Wisconsin--Madison, 1967. / Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph. D.)--University of Florida, 1974. / Description based on print version record. Typescript. Vita. Includes bibliographical references (leaves 136-141).

M.Sc. (Botany) / Please refer to full text to view abstract

No description available.

<i>Trichoderma harzianum </i>and <i>Aneurinobacillus migulanus </i>were tested separately and in combination as potential biological control agents against <i>F. oxysporum </i>f. sp. <i>gladioli </i>on gladiolus in soil-less culture.  The major secondary metabolites produced by <i>Trichoderma harzianum </i>against <i>F. oxysporum </i>f. sp. <i>gladioli </i>in the treated gladiolus corms as well as in the potato dextrose broth were studied.  The impact of re-application times on the effectiveness of <i>Trichoderma harzianum </i>and <i>Aneurinobacillus migulanus </i>against gladiolus corm rot and Gladiolus production in soil-less culture was examined. Results showed that <i>T. harzianum </i>proved to use different mechanisms against <i>F. oxysporum </i>f. sp. <i>gladioli </i>depending on  the inoculation method. <i>T. harzianum </i>and <i>A. migulanus </i>reduced host defence enzymes.  Also, they increased GA ₃ and IAA content in the corms.  SEM showed that the suppressive mechanisms of <i>T. harzianum </i>and <i>A. migulanus </i>differed <i>T. harzianum </i>appeared to operate through a combination of antibiosis and substrate competition, 6-n-pentyl-6H-pyran-2-one (6PP) and harzianic acid (HA) were found as the main metabolites secreted by <i>T. harzianum.  </i>A significant positive correlation was revealed between qPCR and CFU for both <i>T. harzianum </i>and <i>F. oxysporum </i>f. sp. <i>gladioli.  </i>Multiple applications of <i>T. harzianum </i>and <i>A. migulanus </i>re- application was a successful strategy for suppression of <i>F. oxysporum </i>f. sp. <i>gladioli.  </i>Briefly, <i>T. harzianum </i>was more effective than <i>A. migulanus </i>in <i>F. oxysporum </i>f. sp. <i>gladioli </i>suppression and Gladiolus growth enhancement in soil-less culture. The mixing strategy between <i>T. harzianum </i>and <i>A. migulanus </i>failed.

No description available.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1950. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 80-81).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1970. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliography.

There is at present a growing concern and awareness of the endangered status of many indigenous South African plants in the wild, a number of which have potential for commercial production. One such example is Gladiolus scabridus, a vulnerable species endemic to the mountains of northern KwaZulu-Natal and southern Swaziland. It has considerable potential due to its floral characteristics. However, little is known about its horticultural requirements. Thus propagation and cultural practices were investigated with the aim of both conservation and commercialisation of the species. The ideal conditions for G. scabridus seed germination were determined. The presence or absence of light had no significant effect. Optimum germination was achieved at 20 QC of both winged and dewinged seeds. Higher temperatures appear to have a negative effect on germination and none of the winged seeds germinated at 30 QC. Under ideal conditions, fresh seed showed significantly higher vigour and viability than stored seed although the germination of stored seed was hampered by a higher internal fungal content. Successful tissue culture protocol was established for G. scabridus axillary bud and cormel halve explants. During the initial shoot initiation and proliferation stages (stage I and 11), the significantly higher shoot numbers occurred in the absence of growth regulators in both explant types. However, shoots with 1.0 mg 1 -1 6-benzyl-amino-purine (BAP) and 0.5 mg 1 -1 1-naphthalene-acetic-acid (NAA) were healthier in appearance. Higher levels of 5.0 mg 1 -1 NAA inhibited shoot production and encouraged root development in cormel halve explants. During stage 11, axillary bud explants showed root and cormlet development. More roots were initiated without growth regulators, whilst 5.0 mg 1 -1 NAA resulted in significantly better cormlet development. Shoot and cormlet growth of cormel halve explants during stage II was not significantly affected by the presence or absence of NAA and BAP. Significantly more roots were produced with 5.0 mg 1 -1 NAA. During stage III (rooting), the presence of activated charcoal (AC) was essential for the initiation and development of roots in vitro. Root and cormlet development in all explant types was significantly affected by the interaction between the previous treatments from stages I and 11 and the new treatments. During stage IV (hardening off) , most explants died down in the hardening off media leaving resting cormlets. There was a significant interaction between 1.0 mg 1 -1 NAA and 0.3 % AC from stage III, resulting in significantly more cormlets in both axillary bud and cormel halve explants. There was successful cormlet growth after cold storage which is advantageous in reducing the need for acclimatization. G. scabridus corms were successfully forced out of their normal flowering period. Temperatures of 10,5.5 and 2 QC showed successful corm initiation although the corms need to be stored for longer at warmer temperatures. It is suggested that a treatment of 2 QC for 6 weeks is the optimal condition for forcing G. scabridus corms. Although originating from a stressful environment, G. scabridus appears to show capacities for improved growth under controlled conditions. Fertilizer applications enhanced growth and reduced the time to flowering. Nitrogen (N) was found to be important for vegetative growth, flowering and daughter corm development, whereas potassium (K) influenced cormel production. Fertilizer with higher N and lower K is appropriate for the beginning and middle of the growing season and then adjusted to a lower N and higher K fertilizer to promote cormel formation. G. scabridus was found to produce prolific numbers of cormels which is an important source of plant material. Mineral leaf analysis showed that optimum levels for wild species are lower than those for hybrid gladioli with the optimum levels affected by physiological corm maturity and subsequent plant growth. Norms for postharvest handling of cut G. scabridus spikes have been developed. Spikes held in 2 % sucrose had a longer vase life and better floret opening and quality than those kept in distilled water, Prolong, Chrysal, 2 % sucrose and 2 % ethanol, 1 % sucrose, 4 % sucrose, 1 % sucrose and 0.5 % JIK, 2 % sucrose and 0.5 % JIK and 4 % sucrose and 0.5 % JIK, 2 % fructose and 2 % glucose. The use of commercially available solutions should be used with caution. Florets produced a climacteric-like CO2 peak, but levels of ethylene were unmeasurable. Cold storage and the use of polypropylene sleeves delays senescence. G. scabridus spikes secrete droplets of a sticky substance which was confirmed to be extra floral nectar through HPLC analysis. Market research revealed a positive response to the species from consumers and retailers alike with potential for cultivation as a cut flower and bedding plant. However, the cost will determine supply and demand. A field study conducted at Bivane Dam, northern KwaZulu-Natal, confirmed that G. scabridus colonies prevail in rocky, quartzite outcrops where they become wedged between the rocks. Plants were found at different stages of development with populations of not more than 108 plants per colony. Soil data of G. scabridus sites was compared to that of two sites nearby. It was found that G. scabridus soils are higher in phosphorus (P), zinc (Zn) and organic carbon. Leaf analysis confirmed that they have adapted their growth to low nutrient levels. The G. scabridus studies have clearly shown that the species can be successfully moved from a wild plant to a commercially viable one and in so doing its conservation status can also be improved. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2005.

Made available in DSpace on 2016-02-26T12:59:37Z (GMT). No. of bitstreams: 1 MarceloGancedo.pdf: 341813 bytes, checksum: 7979c040bfdc034605ce046a6a950ead (MD5) Previous issue date: 2007-03-29 / Conselho Nacional de Desenvolvimento Científico e Tecnológico / This work was developed in Gardening at UFMS (Universidade Federal de Mato Grosso do Sul) NCA (Núcleo Experimental de Ciências Agrárias), from September 2004 to February 2006 having the aim of valuating nitrogen, limestone and phosphogypsum over gladiolus development and over some chemical and physical characteristics of a dystroferric red latosol. Plants were valuated by leaf analysis, maximum high, spike and floral stem length, new corm diameter, dry and live mater form shoot area, and produced cormel number. Soil physical characteristics valuated the meaning clay, the meaning clay disperse in water, flocculation degree, aggregation and aggregation stableness. Soil chemical characteristics considered water and CaCl2 pH, macro and micronutrients meaning, exchangeable acidity, effective kation exchange capacity and base saturation. The experiment analysis was done in randomized blocks sub-subdivided in parcels, considering as parcel the nitrogen proportions (150, 300 kgha-1), as sub parcel the limestone proportion (0, 2000, 4000, 8000 kgha-1) and as sub-sub parcel phosphogypsum proportion (0, 500, 1000, 2000 kgha-1), in four repetitions. Nitrogen, limestone and Phosphogypsum proportions used caused no visual deficiencies detected in gladiolus. The smaller flowering time and the bigger live matter plants were gained without using limestone and / or phosphogypsum. Limestone between 6000 and 8000 kg.ha-1, contributed into ideal pH levels / Este trabalho foi desenvolvido na área de Jardinocultura do Núcleo Experimental de Ciências Agrárias da UFMS, durante o período de setembro de 2004 a fevereiro de 2006 tendo por objetivo a avaliação dos efeitos do nitrogênio, calcário e gesso agrícola sobre o desenvolvimento de Gladiolus grandiflorus e sobre alguns atributos físicos e químicos de um Latossolo Vermelho distroférrico. As plantas foram avaliadas por meio de análise foliar e quanto à altura máxima, comprimento da espiga e da haste floral, diâmetro do novo cormo, matéria fresca e seca da parte aérea e número de cormilhos produzidos. Como atributos físicos do solo foram avaliados o teor de argila, teor de argila dispersa em água, grau de floculação, agregação e estabilidade dos agregados. Os atributos químicos considerados foram o pH em água e em CaCl2, teores de macro e micronutrientes, acidez trocáveis, capacidade de troca de cátions efetiva e saturação de bases. O experimento foi analisado como inteiramente casualizado, em parcelas sub-subdivididas, sendo consideradas como parcelas as doses de nitrogênio (150 e 300 kg.ha-1), como subparcelas as doses de calcário (0, 2000, 4000 e 8000 kg.ha-1) e como sub-subparcelas as doses de gesso (0, 500, 1000 e 2000 kg.ha-1), com quatro repetições. As doses de nitrogênio, gesso e calcário utilizadas não induziram Deficiências detectadas visualmente no gladíolo. O menor tempo de florescimento e a maior matéria fresca das plantas de gladíolo foram obtidos sem o uso de calcário e/ou de gesso. As doses de calcário entre 6000 a 8000 kg.ha-1, contribuíram para obtenção de níveis ideais de pH em água para o gladíolo. A dose de nitrogênio de 300 kg.ha-1, promoveu a redução do pH, Ca e Mg e de argila dispersa em água e aumentou o grau de floculação e os teores de Al.