There has been interest in developing a cashew industry in Maputaland, the far north-eastern corner of Natal/KwaZulu. Flowering and fruit development coincide with a rainy period, with accompanying serious flower diseases (Oidium anacardii and Colletotrichum gloeosporioides). Glasshouse studies were carried out at Pietermaritzburg, concurrently with field trials in Maputaland, in an attempt to manipulate flowering and growth of cashew trees. Two glasshouse trial were carried out. A factorial design with treatments 0, 3, 6 and 9 weeks of low temperatures (24°C day/9°C nights)(factor A) and 0, 3, 6 and 9 weeks of water deficit (Factor B) was used, with both factors in all combinations. During the second season the durations were increased to 0, 4, 8 and 12 weeks for both factors. No flowering occurred in this trial. Tree growth was not affected significantly by drought and/or cold duration. Temperature appeared to be the dominant factor at low temperatures, stomatal conductance and transpiration being suppressed by cold regardless of soil water potential. At more optimum temperatures for growth, stomatal conductance was dependent on soil water potential (r² = 0.756). Starch levels in the roots, dry matter production in the leaves, roots and stems, as well as leaf area were decreased significantly (P≤0.01) with increasing low temperature duration. Another glasshouse trial to test the effects of foliar urea at concentrations of 0, 1, 2, 4 and 8 g urea 100 l⁻¹, applied once, twice or thrice at fortnightly intervals was undertaken. The treatments were applied in late autumn/early winter of 1990 and 1991. Tree growth and flowering were monitored, and starch and leaf NH₃/NH₄⁺ analyses carried out. The highest urea concentration (8%) resulted in leaf scorch and abscission, extremely low stem diameter growth rates, and was too high for glasshouse trees. The starch contents of the 8% urea treatment were depleted significantly (P≤0.01) more than the other concentrations. The other urea treatments resulted in vigorous growth and high dry matter production. There were no significant effects of the number of sprays on cashew growth. Only seven trees flowered, and therefore no definite conclusions could be drawn regarding urea effects on flowering. Most hermaphrodite flowers (max. 76.8% hermaphrodite) opened soon after first anthesis of a panicle, and all terminal flowers of panicle branches were hermaphrodite. Flowers generally opened basipetally in a panicle, starting with hermaphrodite flowers and with progressively more male flowers. Urea sprays resulted in NH₃/NH₄⁺ build-up in the leaves, concentrations in flowering trees ranging from 100 to 700 μg g⁻¹ DM for approximately a month. A field trial at was carried out at Makatini Research Station to determine the effects of timing of a two month winter drought period on flowering and growth. An observational trial to determine the effects of girdling on growth and flowering was incorporated in the border rows of the irrigation trial. The trial tested five treatments (no irrigation during May and June, June and July, July and August, August and September, and a control treatment which received irrigation throughout Winter). Mean monthly temperatures were below 20°C, and mean minimum temperatures below 15°C for the 5 winter months during treatment application. There were no significant differences in tree growth, flowering, flushing, or yields between drought stressed treatments and control, indicating that, under the conditions at Makatini, autumn and winter temperature was the overriding factor controlling initial flower induction. Flowering occurred from early October (when mean temperatures exceeded 23 to 24°C) to late April (7 months - a prolonged flowering period), when mean monthly temperatures dropped below 23 to 24°C. Girdling of cashew trees in March and May, using girdle widths of 1, 5 and 10 mm was not successful in improving flowering and yields under the conditions of the trial. A field trial was carried out at Mosi Estate in Maputaland to test the following chemicals as tree and/or flower manipulators: foliar applied ethephon (50,100,200,500,2000 mg l⁻¹), KNO₃ (1 %, 2%, 4%), urea (1 %, 2%, 4%) and paciobutrazol (500, 1000, 2000 mg l⁻¹). A phenological model for cashew in Maputaland showed a dormant period during winter, followed by a generative flush, from which panicles and flowers were produced (peak November-January). The harvest period peaked in February and March. A strong post-harvest flush preceded the winter dormant period. Trunk starch levels were at their highest after the dormant winter period, and at their lowest following the harvest. Ethephon at high concentrations (500 and 2000 mg l⁻¹) resulted in excessive leaf drop, disturbed the root shoot balance and normal phenological patterns, and gave poor yields. The best ethephon concentrations were 100 to 200 mg l⁻¹. KNO₃had no significant effect on tree growth, flushing, flowering or yields when compared to control trees. Urea at 2% concentration gave a significant increase (P≤0.05) in flushing and simultaneous decrease in flowering. Paclobutrazol at 500 to 2000 mg l⁻¹ resulted in significantly lower growth rates, and early panicle production. The mean yields of all paclobutrazol treated treatments were higher than controls, despite a hail storm which damaged the flowers. From results of this trial, the use of these chemicals to improve yields and manipulate flowering may not be economically justified. The most promising chemical for further research was paclobutrazol. / Thesis (M.Sc.Agric.)-University of Natal, Pietermaritzburg, 1994.

Propagating methods for peach (Prumis persica L. Batsch.) are currently limited to the use of seeds or cuttings. Most of the rootstocks commonly used for establishing peach trees commercially originate from a narrow genetic base. The most commonly used peach rootstock in South Africa is 'Kakamas', which has disadvantages such as slow growth, and a high susceptibility to certain pests and diseases. 'Kakamas' is classified as a mid to late ripening cultivar, hence, its use as a rootstock is restricted in early ripening cultivars. Optimising peach seedling and cutting production may on the other hand increase more selection material that will increase genetic variability and also serve as the basis for future production and selection of rootstocks for peach nursery establishments. Hence, this study was designed to improves success of peach cutting production by increasing the rooting percentage and the survival rate of cuttings. This was firstly achieved by the application of various concentrations of IBA and several rooting-cofactors. Secondly, rooting success of cuttings taken at different seasons was studied. The response of two cutting positions to various IBA and rooting-cofactor was evaluated. Attempts were also made to overcome the requirement of peaches for a seed stratification period by using plant growth regulators. Studies to optimise the establishment and growth of embryos of early to late ripening cultivars ('Klara', 'Oom Sarel' and' Summer Giant') in vitro were undertaken to overcome the problem of small underdeveloped embryos which are often obtained from early ripening peach cultivars. The early ripening peach cultivars 'DeWet', 'Earlibelle' and 'Florida Prince' were propagated by softwood, semi-hardwood and hardwood cuttings prepared from August 1999 to July 2000. It was found that softwood cuttings prepared in October and November responded well to an IBA application of 1000 mg 1(-1), resulting in 100 % rooting in all three cultivars. In 'Florida Prince' and 'DeWet' the rooting-cofactors chlorogenic acid and phloroglucinol at the concentration of 100ug 1(-1) yielded 77 % and 81 % rooting, respectively while quercitin and rutinin gave only 46 and 44 % rooting for all cultivars. The basal portion of the cutting rooted better than the terminal portion (78 % versus 58 %). Rooting percentage differed in all treatments in response to IBA application and rooting-cofactors 74 % for 'Florida Prince', 62 % for 'DeWet' and 54 % for 'Earlibelle'. The use of IBA and Ca-EDTA proved to be beneficial for rooting of 'Florida Prince' cuttings and resulted in a rooting percentage of 86 % in wounded hardwood cuttings of this cultivar. It was also shown that the simple sugars glucose, fructose and sucrose as well as the sugar alcohols sorbitol and mannitol accumulated at the base of the cutting during adventitious base root formation if the cutting bases were treated with 1000 mg 1(-1) IBA. The concentration of these sugars and sugar alcohols were lower in untreated cuttings compared to IBA treated cuttings during the adventitious root formation process. Studies on the origin of adventitious root formation in stem cuttings of peaches were conducted using light microscopy and transmission electron microscopy. These studies revealed that adventitious roots originate (in peaches) in the vicinity of the vascular bundle tissue and in the cells around them. It was found, however, that adventitious root formation in IBA treated cuttings is associated with the formation of root primordia. These may trigger root initiation and ultimately the development of adventitious roots. In embryo culture studies the highest number of roots and greatest length of roots per embryo as well as the highest number of embryos forming roots was achieved when either the medium of Murashige and Skoog (MS) (1962) or of Steward and Hsu (SH) (1978) were employed. The Woody Plant medium (Lloyd and McCowan, 1978) and the medium after Schenk and Hildebrandt (1972) were found to be less effective when compared to MS and SH media. Addition of GA(3) (0.01 mg 1(-1)), BAP (0.2 mg 1(-1)) or GA(3) (0.01 mg 1(-1))+ BAP (0.2 mg 1(-1))+IBA (0.5 mg 1(-1)) to the media gave the best results with respect to embryos forming roots (86 %), number of roots per embryo (8.0) and total length of roots per embryo (7.57 cm). Furthermore, the following conditions for optimal rooting of peach embryos were established: pH 5.2, 1.5 g 1(-1) agar, 60 g 1(-1) sucrose and 16h light/8h darkness photo period. Further studies on the influence of the stratification temperature on germination of embryos revealed the highest germination percentage after exposure to 40 C ±2 constantly for 30 to 60 days. However, this chilling requirement can be successfully substituted by the addition of GA(3) , kinetin, zeatin, BA and thiourea to the culture media. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 2001.

Avocados are becoming an increasingly important crop in South Africa, where the main producing areas include Limpopo, Mpumalanga and KwaZulu-Natal provinces. The South African avocado industry faces considerable challenges including increasing competition exporting avocados, particularly to the European market. The processes involved to export avocados has markedly improved over the past two decades, however there is always room for improvement and it is necessary to remain competitive on a global scale. Issues such as fruit being partially soft on arrival, quality defects and cold chain management breakdown are still present. It is necessary to investigate new aspects of cold storage such as extending the storage period and understanding the physiological aspects involved. To improvement such issues, an investigation was conducted on ultra-low temperature shipping (1°C) as well as the use of 1-MCP; the implementation of deliberate cold chain breaks to achieve a better understanding as to the quality influences involved; an extended storage period of 56 days to assess the quality issues and benefits involved; as well as investigating the physiological aspects involved with all above treatments on 'Hass' avocados. An initial study saw early-, mid- and late-season 'Hass' avocados stored at 1°C or 5.5°C for 28 days. Additional treatments included fruit treated and not treated with 1-MCP as well as waxed and unwaxed fruit. Storage at 1°C was comparable with 1-MCP treatment for both fruit softening in storage and extending the ripening period. Storage at 5.5°C resulted in partial in-transit ripening, if 1-MCP was not used. Early-season fruit incurred the most external chilling injury but overall levels were minimal and not concerning. Mid-season fruit were the most sound in terms of quality. It is suggested that 1°C can be used as a viable economic alternative to 1-MCP for long distance shipping of 'Hass' up to 28 days. The cold chain break trial included a 24 hour delay before cold storage, a deliberate 8 hour break at day 14 of cold storage where fruit were removed from cold storage and a control of 28 days cold storage where no break was involved. Early-, mid- and late-season 'Hass' avocados were stored at 1°C or 5.5°C for 28 days. Additional treatments included fruit treated and not treated with 1-MCP as well as waxed and unwaxed fruit. It was found that cold chain breaks do influence the amount of water loss, fruit softening and days taken to ripen. Storage at 1°C did not entirely negate the effects of cold chain breaks compared with 5.5°C, but did result in fruit which were harder at the end of storage and took longer to ripen. The use of 1-MCP also had advantageous effects with respect to significantly lengthening the ripening period, even when a cold chain break occurred, compared with fruit not treated with 1-MCP. As results of the study differed in some respects to those of previous studies, it is recommended that further work be conducted to determine what fruit or pre-harvest factors affect the fruit physiological changes which take place when cold chain breaks occur. Having the option to make use of an extended storage period would be of benefit to the industry if delays occur and fruit have to be maintained under cold storage. Extended storage of South African avocados, especially at the end of the season would also allow for the option of strategically holding back fruit from the export market in order to extend the supply period. It would not only benefit export options, but would also be highly beneficial to local pre-packers, as it would reduce the need to import fruit from the Northern hemisphere production areas during the South African off-season. Early-, mid- and late-season 'Hass' avocados were stored at 1°C or 5.5°C for 56 days. Additional treatments included fruit treated and not treated with 1-MCP as well as waxed and unwaxed fruit. The combination of 1°C with the use of 1-MCP resulted in a good shelf life as well as maintenance of internal quality and integrity. External chilling injury is of concern for early-season fruit, however, mid- and late-season fruit did not incur extensive damage. It is, therefore, advised that fruit placed in extended storage are marketed through the 'Ready ripe' program to mask any chilling injury on the 'Hass' fruit. Avocados are renown as a "healthy food" due to their nutritional value as well as containing relatively high concentrations of antioxidants. The fruit also contain high amounts of C7 sugars which can act as antioxidants. Additionally, C7 sugars and other antioxidants play important roles in fruit quality. Therefore, it is important to understand how varying storage conditions and treatments affect the levels of these physiological parameters. Treatments of cold chain break/delay included a deliberate 8 hour break at day 14 of cold storage where fruit were removed from cold storage, a 24 hour delay before cold storage and a control of 28 days where no break was involved. A 56 day extended storage period was also used. Early-, mid- and late-season 'Hass' avocados were stored at 1°C or 5.5°C for 28 days. Additional treatments included fruit treated and not treated with 1-MCP as well as waxed and unwaxed fruit. The use of 1-MCP maintained higher levels of antioxidants, ascorbic acid and C7 sugars for both the 28 day and the 56 day storage periods. The 24 hour delay had a tendency to increase consumption of anti-oxidant and sugar reserves. The use of 1°C resulted in antioxidant and ascorbic acid levels decreasing while maintaining higher sugar levels. Overall, high stress imposed on fruit decreased reserves resulting in poor quality fruit. The use of 1°C and 1-MCP treatments maintained fruit quality. / Thesis (M.Sc.Agric.)-University of KwaZulu-Natal, Pietermaritzburg, 2011.

Avocados are an important horticultural crop in South Africa, especially in the provinces of KwaZulu-Natal, Mpumalanga and Limpopo. The distance to traditional export markets, phytosanitary restrictions to lucrative markets such as China, the USA and Japan and increased competition in the European market have challenged the South African avocado industry. The industry has responded with improved logistics and shipping, a co-ordinated market access program and a global system to co-ordinate exports of avocados to the European market. To remain competitive on the global market, further improvements and innovations are required to improve the efficiency of postharvest operations. These improvements and innovations should be guided by a greater understanding of postharvest physiology. Avocados are a relatively new export crop, so there is still much to be learnt about avocado postharvest physiology and the optimisation of postharvest management. In this regard, reduced temperature storage (1°C) and modified humidity packaging (MHP) were investigated for their effect on fruit physiology and quality, the effect of a water- and ABA-infusion on ripening was examined and the effect of a cold chain break on fruit physiology and quality determined; near-infrared spectroscopy was also examined for its potential for its use in the avocado industry. As an initial study, the relationships between individual sugars, protein and oil were studied to understand the changes in avocado fruit during ripening. It was found that mannoheptulose and perseitol were the predominant sugars at harvest, but declined to very low levels during the first 10 days postharvest. The concentrations of glucose and fructose increased, while sucrose declined slightly during ripening. The concentration of protein increased sigmoidally during ripening, reflecting the increase in the ripening enzymes, particularly cellulase and polygalacturonase. The oil content fluctuated slightly during ripening. It is suggested that mannoheptulose and perseitol are important carbon and energy sources during ripening. Glucose concentration was also found to increase earlier in fast ripening fruit compared to slow ripening fruit, which is related to increased cellulase activity and may be related to the ABA functioning. Thereafter, storage and ripening trials in two consecutive seasons showed that 1°C storage and the use of MHP for 28 days reduced mass loss, water loss, ethylene production, respiration, softening and heptose consumption, without appreciably affecting fungal rots, physiological disorders or external chilling injury, compared to fruit stored at 5.5°C and regular atmosphere respectively. Also, the storage of fruit in MHP delayed the rise in the activity of cellulase during ripening, compared to fruit not stored in MHP, but there was no significant difference in the peak activity of cellulase, polygalacturonase or pectin methylesterase. In a separate experiment, fruit ripening was significantly affected by the infusion of ABA in an aqueous solution. Water slightly reduced the variation in ripening while ABA reduced the time to ripening and the variation; it is suggested that water stress and ABA are intrinsically involved in the ripening processes and may act as a ripening trigger. The water concentration in fruit was measured non-destructively using reflectance NIR; this model was used to determine the maturity of fruit and the loss of water during cold storage. In the cold chain break experiment, it was found that although fruit recovered after a cold chain break, in terms of ethylene production and respiration, there was a loss in quality because of severe shrivelling as a result of increased water loss. Fruit that were stored at 1°C were generally of a better quality at ripeness, if the cold chain was broken, compared to fruit stored at 5.5°C. In a follow-up experiment, it was found that significant changes occurred in avocado physiology over a 6h period. The respiration rate of fruit significantly increased after 4h at room temperature and mannoheptulose declined by 32% in control fruit and by 16% in ethephon-treated fruit after 6h. This demonstrates the potential for quality loss in a short amount of time. Furthermore, a model of avocado ripening is proposed, outlining the role of water, ABA, ethylene, respiration, ripening enzymes and individual sugars. This study has contributed to the understanding of avocado postharvest physiology and should aid in better management of avocados for improved fruit quality and consumer satisfaction. / Thesis (Ph.D.Agric.)-University of KwaZulu-Natal, Pietermaritzburg, 2011.

The South African avocado industry is export-orientated with forty percent of total production sold overseas. The avocado fruit is a highly perishable product with a relatively high rate of respiration which results in the quick deterioration of fruit quality. Good phytosanitary procedures are a necessity in ensuring good product quality. Due to the threat of pests and diseases becoming resistant to the conventional chemicals currently used to control them, there has been a great need to diversify from their usage. Silicon (Si), being the second most abundant element (28%) in the earth’s crust after oxygen, is a major constituent of many soils and has been associated with disease resistance in plants for a long time. It has been used in a number of crop species to provide resistance against pathogenic agents. In some horticultural crops Si has been found to offer protection against fungal infections by strengthening cell walls, thus making it more difficult for the fungi to penetrate and colonize the plant. The aim of this research was to investigate the effects of postharvest silicon application on the quality of ‘Hass’ avocado fruit. The specific objectives included investigating the effect of silicon on the ripening pattern as well as the metabolic physiology of the avocado fruit. Avocado fruit were obtained from two locations in the KZN Midlands (Everdon Estate in Howick and Cooling Estate in Wartburg). Fruit were treated with different forms of Si (potassium silicate (KSil), calcium silicate (CaSil), sodium silicate (NaSil) and Nontox-silica® (NTS)) at concentrations ranging from 160 ppm to 2940 ppm. After dipping for 30 minutes in the silicon treatments, the fruit were stored at -0.5°C, 1°C, 5°C or at room temperature (25°C). Energy dispersive x-ray (EDAX) analysis was then conducted on the exocarp and mesocarp tissues to determine the extent of silicon infiltration within each treatment. Firmness measurements, ethylene evolution and CO2 production were recorded as fruit approached ripening. The CO2 production of fruit that were stored at room temperature was analysed daily until they had fully ripened, while fruit from cold storage were removed weekly to measure respiration. Mesocarp tissue from each fruit was extracted using a cork borer and subsequently freeze-dried and stored for physiological analysis. The freeze-dried mesocarp tissue was then finely ground and later analysed for sugar content, total anti-oxidant capacity (TAOC), total phenolic (TP) content and phenylalanine ammonia lyase (PAL) activity using their respective assays. Statistical analyses were carried out using GenStat® version 11 ANOVA. Treatment and storage temperature means were separated using least significant differences (LSD) at 5% (P = 0.05). The experimental design in this study was a split-plot design with the main effect being storage temperature and the sub-effect being treatments. Each replication was represented by a single fruit. EDAX analysis revealed that Si passed through the exocarp into the mesocarp tissue in fruit treated with high concentrations of silicon, i.e., KSil 2940 ppm. Significant differences (P < 0.001) were observed in temperature means with regards to firmness. Fruit treated with KSil and NTS only and stored at 5°C were firmer than fruit stored at other temperatures. Fruits treated with Si in the form of KSil 2940 produced the least amount of CO2, while non-treated fruits (Air) had the highest respiration rate. Fruit stored at room temperature (25°C) produced significantly higher amounts of CO2 and peaked much earlier than fruit stored at other temperatures. Ethylene results showed that there were differences (P < 0.05) between temperature means with the highest net ethylene being produced by fruit stored at 25°C. There were also significant differences amongst treatment means (P < 0.001), with fruits treated with KSil 2940 ppm producing the least ethylene. There were significant differences (P < 0.001) in temperature means with regards to the total phenolic concentration with fruits stored at 1°C having the highest TP concentration (26.4 mg L-1 gallic acid). Fruit treated with KSil 2940 ppm had the highest total phenolic concentration whilst the control fruit (Air and Water) had the lowest. There were also differences (P < 0.05) in storage temperature means with respect to the total antioxidant capacity. Fruit stored at -0.5°C had the highest TAOC (52.53 μmol FeSO4.7H2O g-1 DW). There were no significant differences in TAOC (P > 0.05) with regards to treatment means although fruit treated with KSil 2940 ppm and stored at -0.5°C showed the highest TAOC of 57.58 μmol FeSO4.7H2O g-1 DW. With regards to the concentration of major sugars in avocado, mannoheptulose and perseitol (mg g-1), no significant differences (P > 0.05) were observed in temperature means. However, fruit stored at -0.5°C had the highest concentration of these C7 sugars compared with fruit stored at other temperatures. There were significant differences in treatment means (P < 0.001) showing that fruit treated with KSil 2940 ppm had the highest concentration of both mannoheptulose (18.92 mg g-1) and perseitol (15.93 mg g-1) in the mesocarp tissue. Biochemical analyses showed differences (P < 0.05) in storage temperature means with respect to PAL enzymatic activity. Fruit stored at 5°C had the highest PAL activity (18.61 mmol cinnamic acid g-1 DW h-1) in the mesocarp tissue compared with fruit stored at other temperatures. There were significant differences in treatment means (P < 0.001) with regard to PAL activity. Fruit treated with KSil 2940 ppm had the highest PAL activity (23.34 mmol cinnamic acid g-1 DW h-1). This research has demonstrated the beneficial effects, particularly applications of 2940 ppm Si in the form of KSil. This treatment successfully suppressed the respiration rate of avocado fruit. Biochemical analyses of total antioxidants, total phenolics and PAL activity in the mesocarp tissue have shown the usefulness of Si in improving the fruit’s metabolic processes. The C7 sugars (D-mannoheptulose and perseitol) also seem to be more prevalent in avocado fruit treated with Si (particularly KSil 2940 ppm) than in non-treated fruit. This suggests that an application of Si to avocado fruit can aid in the retention of vital antioxidants (C7 sugars). / Thesis (M.Sc.Agric.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

Cabbage is one of the most important and widely grown crops in KwaZulu-Natal (KZN). In cabbage production, no clear cropping methods exist (other than succession cropping) to allow the soil to recover its fertility status naturally after the harvested has crop absorbed nutrients from the soil. Succession cropping system is extensively practiced by cabbage farmers. Succession cropping is when a succeeding vegetable crop is planted immediately after harvesting the preceding crop. This does not allow soil fertility status to be balanced by soil microbes in an environmentally friendly approach. Instead, synthetic fertilizers are heavily used to rectify soil fertility status and these have negative impacts on the soil in the long term, followed by yield decline. However, the economic downturns and green environment awareness have caused researchers and some farmers to focus more on developing environmentally healthy crop production technologies. A priority in this study was to elucidate effects of cover crops-green manure as rotational crops in cabbage production. The experimental site is located at Cedara Research Station. In 2009, soil samples were taken from the top 30 cm of the soil profile, in a zigzag pattern in 5 m x 5 m grids for fertility analysis. Results were used to determine nutrient deficiencies, and blanket application of phosphorus and potassium followed according to each grid’s deficiency. The study was divided into two cycles, with each cycle consisting of season one and season two. The study was conducted over a two year period. Cycle one, season one, 2009/2010 (Dec-Mar): four treatments (sunn hemp: planted at 50 kg/ha seeding rate; forage sorghum: planted at 50 kg/ha seeding rate; mixture of sunn hemp-forage sorghum: each planted at 25 kg/ha seeding rate and control: cabbage planted at 30 cm within rows x 60 cm between rows) were used. The statistical design of the study was a 4x4 Latin Square experiment repeated twice in adjacent fields (347 m2 each) 6 m apart, with a total of 32 plots. Each experimental plot was 8.5 m x 9 m. Genstat Package, 9th Edition was used for data analysis. Standard cabbage production recommendations were followed in control treatments. Cabbage yield was determined in all control plots. Weed coverage percentages were determined in all four treatments. Cycle one, season two, 2010, (Apr-Aug): all 32 plots from season one were each split into four, in a Split Plot design, resulting in a total of 128 plots in both fields. Nitrogen was applied at four different levels: 0, 80, 160 and 240 kg N/ha. Standard cabbage production methods were followed again as in the control treatment in season one. Cover crops were replaced by planting cabbage. Cabbage was repeated in the control plots. Cabbage yield, cabbage leaf nutrients, soil nitrate and ammonium nitrate and soil carbon to nitrogen (C:N) ratio were measured. Cycle two, season one, 2010 (Sep-Dec): The same method in cycle one, season one was repeated. Cycle two, season two, 2010/2011, (Dec-Apr): The same method in cycle one, season two was repeated. Although in this study there was visibly a large amount of residues left over after the trial was harvested, the use of a tractor-drawn slasher was not very effective. The slashed material was not chopped into smaller pieces, as it would if a roll-chopper had been used. A tractor-drawn slasher was used because it is a tool available to many farmers. As a result, it was not a simple process to transplant seedlings mechanically, due to the amount of organic matter that was in the soil after it had been incorporated. The mechanical planter pulled off, removed and became blocked by the residues, and as a result it was not able to operate as it would in normal soils. The residues removed soil from the furrows and that caused seedlings to fall over as all the soil for the root system support had been moved by the planter and stuck residues. The planter had to be assisted by hand planting seedlings that were not properly planted mechanically. Hence, from the operational perspective, the choice of tools for chopping were not effective in this study and this is something to be considered in future studies, where it will be necessary to compare different tools for planting and cover crop incorporation into the soil. During the present study, soil N content in different plots had significant differences (P=0.05). The control showed high levels of total N content, but this was not related to the low yields exhibited by cabbage after cabbage at all levels of N. Since, where there were cover crops, the N content was low, this could mean that N was immobilized, compared to the cabbage treatments where plenty of N was available. Immobilized N is thus not available for leaching, polluting the environment. The yield of cabbage was significantly lowest in controls. The study showed that cover crop-green manure use has a positive effect in terms of yield improvement. The cabbage yields from the sunn hemp plots at a 0 kgN/ha were significantly different from the yield of cabbage planted following cabbage at 240 kgN/ha N. The study also showed that N is not the only yield determinant in cabbage production. Too much N has a tendency to suppress cabbage yields. The cover crop-green manures selected for this study were shown to be ineffective in suppressing weeds. This was true even though the weed coverage percentage in each of the cover crop-green manure plots was above 65%, except for the cabbage crop. This study did not separate different weeds, but the most dominant weeds were Amaranthus hybridus (pigweed), Galinsoga parviflora (gallant soldier), Bidens pilosa (common blackjack) and Cyperus esculentus (yellow nutsedge). These weeds were effectively controlled in the cabbage sole crop by the use of herbicides and mechanical weeding. The result of cover crop-green manures not suppressing weeds was significantly associated with the cover crop-green manure seeding rate. This study has been a starting point in the development of cover crops-green manure as rotational crops in cabbage production. It is concluded that cover crop-green manure (sunn hemp and forage sorghum) used in this study for vegetable (cabbage) production has proven to be successful in improving yields. Yields obtained from cover crop-green manure treatments were better than yields obtained from - conventional tillage methods for cabbage production (control). However, in cabbage, weed suppression and soil N content, failed to improve significantly in response to the cover crop green manures used. The high plant population density under a cover crop, combined with weed population, may lead to high uptake of soil N for plant growth, leaving less N in the soil and in the plant. In the sole cabbage crop, because of low competition, there was adequate uptake of N for plant growth and yet the crop was not exhaustive of soil N. Further research is required in the following areas: comparison of cabbage with other vegetable crops in response to the effect of the selected cover crops; comparison of mechanical weeding and herbicide use as means of controlling weeds during cover crop-green manure growth; identification of dominant weeds in the presence and absence of cover crops, and monitoring of soil water relations and other fertiliser effects. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2012.

Most tomato cultivars used for commercial food production are imported into South Africa. Optimal growing conditions for these specific cultivars need to be determined, as wrong cultivar choices can lead to great financial losses. Lack of information on selecting well-performing cultivars may lead to lower yield or unacceptable fruit quality. Information on the performance of tomato cultivars under South African conditions, utilizing plastic tunnels or shadenet structures under soilless cultivation is still very limited. Soilless cultivation of vegetables is becoming a preferable over in-soil cultivation due to the improved yield and quality of produce, efficient water and nutrients usage by the crop; furthermore, the grower can regulate nutrient solution, electrical conductivity and pH of the nutrient solution. To identify the optimal system for growing tomatoes hydroponically, the performance of four tomato cultivars (‘FA593’, ‘Miramar’, ‘FiveOFive’ and ‘Malory’) under different growing conditions was evaluated: directly planted in soil under 40% shadenet with drip irrigation, a closed hydroponic system under 40% shadenet, an open bag system under 40% shadenet, or an open-bag system in a temperature controlled as well as a non-temperature controlled tunnel. The study revealed that ‘Miramar’ performed better than the other cultivars in all production systems, with the exception of soil cultivation where there were no differences amongst the four cultivars. Fruit cracking was found to be directly correlated with fruit size, as the large-sized cultivars ‘Malory’ and ‘FA593’ were more susceptible than the other two cultivars. Plants grown under shadenet were prone to fruit cracking and raincheck as well as early blight. Higher yields were obtained when plants were produced in the open bag system under temperature controlled conditions and in the closed system under shadenet. Growing tomatoes in the non-temperature controlled tunnel resulted in high incidences of fruit cracking, poor yield and pre-mature fruit ripening probably due to high and fluctuating temperatures under such conditions. The average marketable yield was 88% and 59% of the total yield in the temperature controlled and non-temperature controlled tunnels, respectively. A further experiment was carried out to improve yield and quality of tunnel tomatoes using beneficial micro-organisms, i.e., arbuscular mycorrhiza fungi (AMF) at different nutrient concentrations. Tomato seedlings were treated with Mycoroot™ containing four mycorrhiza species (Glomus etunicatum, Paraglomus occultum, Glomus clarum and Glomus mossea) at transplanting and subsequently transferred to either a temperature controlled or a non-temperature controlled tunnel under the recommended (100%) or reduced (75 and 50%) nutrient concentrations. Sawdust was used as a growing medium in this experiment. Application of AMF neither enhanced plant growth, yield, nor fruit mineral nutrient concentrations; although fruit Mn and Zn concentrations in the temperature controlled tunnel increased significantly following AMF application. Plants grown in the non-temperature controlled tunnel had significantly poorer plant growth, and lower yield and lower fruit mineral concentrations, compared with fruit from plants in the temperature controlled tunnel. Tomato plants in the non-temperature controlled tunnel had higher levels of micro-elements in leaf tissue, compared with those in the temperature controlled tunnel. The highest yields were obtained from plants fertigated with 75% of the recommended nutrient concentration, as compared with the 100 and 50% nutrient concentrations. When coir was subsequently used as the growing medium, Mycoroot™ applied at seeding and transplanting did not enhance mycorrhizal colonization or fruit quality. Growing tomatoes under reduced nutrient supply reduced the total soluble solids in the juice of the fruit, but improved total and marketable yield, as well as the number of marketable fruit. This effect was more substantial in the temperature controlled than in the non-temperature controlled tunnel. Fruit firmness and leaf chlorophyll concentrations were significantly higher in plants grown in the temperature controlled tunnel. Growing tomatoes in sawdust improved the leaf Mn and Ca concentration over that of tomato plants grown in coir. Mycorrhizal colonisation did not have a beneficial effect on tomato yield and quality. The study indicated that cultivar selection was important in obtaining the highest yield and quality of tomato using the closed hydroponic system under shadenet and the open bag hydroponic system in the temperature controlled tunnel. Temperature controlled tunnels with a pad–and-fan cooling system are still an effective way of cooling the tunnel environment which resulted in high yield and high quality of tomatoes with a higher fruit mineral content than that obtained under non-temperature controlled conditions where only natural ventilation is relied on. Results also demonstrated that mycorrhizal colonization in soilless condition has limited beneficial effects in allowing for better nutrient uptake and thereby for improved yield and quality of tomatoes. Further studies, including different media, nutrient composition and concentrations, need to be carried out to investigate the possible causes of AMF failure to improve yield, despite good AMF root colonization. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2013.

There is increasing interest in special heptose carbohydrates, their multifunctional roles from a plant physiological view point in fruit growth and development as well as in the whole plant in general due to their potential in mitigating photo-oxidative injury to the whole plant system and the image of avocado as ‘health fruit’. Studies have been carried out to investigate the role of avocado heptoses, rare carbohydrates predominantly produced in avocado. Several authors have documented various research findings and speculated on multifunctional roles of avocado special sugars. However, few reports have made an attempt to elucidate the multifunctional roles of avocado heptose carbohydrates as: ‘sources of energy’, storage and phloem-mobile transport sugars, and precursors for formation of antioxidants. Assessing the avocado carbohydrates over the plant growth and development during ontogeny may, therefore, offer clues to better understand whole plant behaviour. Plant sampling was carried out over different developmental stages. Using plants grown in the light versus etiolated seedlings; sugar determinations were also done to determine what sugar is produced from which storage organs. The sugars were extracted and analysed by isocratic HPLC/RID. The embryo had 47.11 % hexose and 52.96 % heptose sugars. The seed, however, also released significant amounts of D-mannoheptulose (7.09 ± 1.44 mg g-1 d. wt) and perseitol (5.36 ± 0.61 mg g-1 d. wt). Similarly fruit and leaf tissues had significant amounts of heptoses relative to hexoses at specific phenological stages. In postharvest ‘readyto-eat’ fruit the following carbohydrate concentrations were as follows:exocarp heptoses 13 ± 0.8; hexoses 4.37 ± 1.6 mg g-1 d. wt, mesocarp heptoses 8 ± 0.2; hexoses 3.55 ± 0.12 mg g-1 d. wt), seed heptoses (only perseitol) 13 ± 1.1; hexoses 5.79 ± 0.53 mg g-1 d. wt. The results of this experiment was the first to demonstrate that the heptoses D-mannoheptulose, and its polyol form, perseitol, are found in all tissues/organs at various phenological stages of avocado growth and development. Secondly, heptoses, as well as starch are carbohydrate reserves that are found in avocado. The heptoses, beyond being abundantly produced in the avocado plant, are also found in phloem and xylem saps as mobile sugars. The study also presents data on the interconversion of the C7 sugars Dmannoheptulose and perseitol. It is deduced that D-mannoheptulose can be reduced to perseitol, and perseitol can also be oxidized to D-mannoheptulose by enzymes present in a protein extract of the mesocarp. The potential catalyzing enzyme is proposed to be an aldolase, as electrophoretic determinations prove the presence of such an enzyme during various stages of development in various plant organs. Avocado heptoses play an important role in plant growth and development and in fruit in particular. Moreover, they are reported as sources of anti-oxidants, and contribute significantly to fruit physiology if they function in coordination with other anti-oxidants in fruit tissues. To evaluate the presence of anti-oxidant systems throughout avocado fruit development, various tissues were analysed for their total and specific anti-oxidant compositions. Total anti-oxidant levels were found to be higher in the exocarp and in seed tissue than in the mesocarp. While seed tissues contained predominantly ascorbic acid (AsA) and total phenolics (TP), the anti-oxidant composition of the mesocarp was characterised by the C7 sugar, D-mannoheptulose. Among the anti-oxidant enzymes assayed, peroxidase (POX) and catalase (CAT) were present in higher concentrations than superoxide dismutase (SOD) in mesocarp tissue. Different anti-oxidant systems seem to be dominant within the various fruit tissues. Carbohydrates are the universal source of carbon for cell metabolism and provide the precursors for the biosynthesis of secondary metabolites, for example via the shikimic acid pathway for phenols. The preharvest free and membrane-bound phenols, catechin and epicatechin, are distributed differently in the various fruit tissues. Membrane-bound and free phenols also play a role as anti-oxidants, with free ones being more important. KSil (potassium silicate) application to fruit as postharvest treatment was used to facilitate the release of conjugates to free phenols via lysis. This treatment improved fruit shelf life. Western blotting also revealed that postharvest Si treatment affects the expression of enzymatic anti-oxidant-catalase (CAT). Overall the thesis results revealed that C7 sugars have anti-oxidant properties and that D-mannoheptulose is the important anti-oxidant in the edible portion of the avocado fruit. Dmannoheptulose is furthermore of paramount importance as a transport sugar. Perseitol on the other hand acts as the storage product of D-mannoheptulose, which can be easily converted into D-mannoheptulose. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2009.

‘Fuerte’ makes up 25% of the avocados exported from South Africa to European markets and requires shipping periods of up to 28 days and a correctly managed cold chain. A temperature of 5.5°C and expensive CA and 1-MCP treatments are currently used to delay ripening over this lengthy cold chain; however, fruit still appear on the European market showing signs of softening and physiological disorders. Increased competition on the global market and the disadvantage of a particularly long distance to the European market has challenged the South African export industry. These challenges have necessitated improved road and sea transport logistics, co-ordination with producing countries which supply fruit to European markets over similar periods as South Africa, and research into ultra-low temperature storage to possibly enable future access to new lucrative markets in the USA, China and Japan. It is also known that there are various ‘weak links’ in this cold chain and that cold chain breaks are detrimental to fruit quality, but further research into the negative effects of these cold chain breaks at ultra-low temperatures was needed. Thus, the objective of the study was to determine the potential for shipping ‘Fuerte’ avocados at temperatures of 2°C as well as determining the effects of cold chain breaks on fruit quality, throughout the growing season and possibly for an extended period of 56 days. ‘Fuerte’ avocados were harvested at three different maturity stages reflecting early-, mid- and late-season fruit, with moisture contents of 74%, 68% and 63%, respectively. Fruit were stored at 2°C or 5.5°C, treated with 1-MCP and waxed. Additionally cold chain breaks (24 hour delay and break at 14 days) were implemented. Fruit softening, mass loss, days-to-ripening, external and internal quality as well as antioxidant levels and total sugar levels were determined. The first aim was to determine whether a lower than currently used storage temperature could be a successful alternative to 1-MCP use. A storage temperature of 2°C provided good internal quality as well as reduced mass loss and fruit softening, which is related to the slightly reduced use of C7 sugars at 2°C compared with 5.5°C. Although the overall occurrence of external chilling injury was relatively low, 2°C storage caused a notably higher occurrence of external chilling injury than 5.5°C storage, particularly early in the season, but extended the days-to-ripening. Unfortunately, no correlation between the anti-oxidants in the exocarp and external damage was found. Waxing significantly reduced the external damage on fruit stored at 2°C, so much so, that the treatment combinations of ‘2°C, no 1-MCP, waxed’ showed no external chilling injury throughout the season. Further, waxing fruit at 2°C could eliminate the need for 1-MCP, delivering a product of the required shelf-life and quality. Best results were achieved for mid-season fruit stored at 2°C. Late-season fruit would potentially be the most profitable to store at this low temperature, however, body rots (anthracnose and stem-end rot) were more common in the late-season. Storage at 2°C can therefore maintain the internal quality over a storage period of 28 days and be a potential alternative to 1-MCP use as the season progresses. The effect of cold chain breaks on fruit quality was then investigated and showed that both a delay and a break in the cold chain increased mass loss and fruit softening, reduced days-to-ripening and increased external chilling injury, especially early in the season. Water loss was the main contributor to the decreased fruit quality which resulted from the delay in cooling, increasing external damage significantly, particularly early in the season. The break at 14 days had a marked effect on physiological activity of fruit during storage, seen mainly in the increased metabolic activity, resulting in increased fruit softening and water loss during storage and a decrease in C7 sugars and thus shelf-life, particularly for fruit stored at 5.5°C. Importantly, 1-MCP use and storage at 2°C reduced the effects of cold chain breaks with respect to fruit softening, however, lowering the storage temperature had a greater negating effect than 1-MCP and could be a successful alternative to the use of 1-MCP. The internal quality throughout the experiment was very good, with few internal disorders and no significant treatment effects on internal quality and C7 sugar concentrations. Overall, a break in the cold chain, before and during cold storage, resulted in a marked reduction in fruit quality. The storage temperature of 5.5°C should not be used for a 56 day storage period as it resulted in significant fruit softening during storage, even when 1-MCP was used, and resulted in significantly more external chilling injury in the mid- and late-season than at 2°C. Storage of 1-MCP treated, waxed fruit at 2°C, resulted in the best shelf-life and fruit quality, particularly mid-season fruit which had negligible external chilling injury and 100% sound fruit. Early-season fruit suffered significant external chilling injury at 2°C and late-season fruit had the highest body-rots and internal disorders at this storage temperature. Although mid-season fruit could be successfully stored at 2°C for 56 days, the use of a 56 day storage period is not recommended as a practical storage period, due to the high risk of external damage, particularly if maturity levels are not optimum and trees and fruit are not of the highest quality. Overall this thesis has shown that 1-MCP treatment can play an important role early in the season when fruit are susceptible to external damage, however, storage at 2°C results in good quality fruit and, when used in conjunction with waxing, appears to be a viable alternative to the use of 1-MCP, particularly later in the season. Further, the negative effects of cold chain breaks on fruit quality have been demonstrated and, importantly, the storage temperature of 2°C negates the fruit softening effects of these breaks, even if 1-MCP is not used. / Thesis (M.Sc.Agric.)-University of KwaZulu-Natal, Pietermaritzburg, 2011.

The use of sulphur as a method of postharvest disease control and colour retention in litchis is soon to be restricted by the European Union. It is therefore essential that new postharvest treatments and packaging techniques be developed in order to retain internal and external fruit qualities and thus allow for export. Good litchi quality is not only important for the export market but also for use on the local market. In this study, alternative methods for postharvest quality control were investigated with the aim of extending the litchi storage life to 40 days under modified storage. Packaging the fruit in polypropylene bags significantly decreased fruit water loss and resulted in an increase in shelf life, as determined by red colour and overall rind appearance. There was no distinct advantage of amodified atmosphere. The use of a punnet, lined with absorbent sheeting and placed within the sealed polypropylene bag, further improved the shelf life. The absorbent sheeting reduced the amount of free water and resulted in little pathogen infection, while the punnet was effective in protecting the fruit from damage. It was notable that most water loss occurred within the first 10 days of storage and that the majority would actually take place during the cooling phase. A hydrocooling technique was therefore investigated and was found to not significantly decrease water loss, possibly due to not hydrocooling the fruit for a long enough period of time. Temperature management was extremely important for both colour retention and pathogen control. It was found that treatments stored at 5.5QC showed better colour retention after the 40 days storage than the 1QC storage treatment. The higher storage temperature, however, enhances the potential for postharvest diseases. Three compounds, namely ISR 2000, 'Biosave' and F10, were tested for pathogen control. 'Biosave' showed the best results with the most effective concentration being 100 mill water and good pathogen control occurred when storage was at 10 C. Polyphenol oxidase (PPO) activity in the litchi rind was evaluated as it is thought to be closely related to browning of litchi fruit, probably due to the degradation of phenolics by PPO. Brown fruit had a high PPO activity whilst red fruit had much lower activity. It was also shown that PPO activity decrease over storage time, possibly due to product inhibition of the enzyme. The internal quality of the fruit was determined using the T88: acid ratio of the pulp, as it is well correlated to mean eating quality. For fruit to have excellent taste, it must have a T88: acid ratio of between 31:1 and 60:1. All the fruit had a ratio that met this criterion and would therefore ensure good eating quality. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.